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The map label for this gene is queA

Identifier: 85860046

GI number: 85860046

Start: 2257507

End: 2258595

Strand: Direct

Name: queA

Synonym: SYN_00039

Alternate gene names: 85860046

Gene position: 2257507-2258595 (Clockwise)

Preceding gene: 85860045

Following gene: 85860047

Centisome position: 71.01

GC content: 54.27

Gene sequence:

>1089_bases
TTGATGAAGCTTCAAGACTTCGATTACCAGCTTCCCCAGTCCCGGATTGCTCAGAGCCCCTGCGCAAAGCGGGATCATGC
CCGCATGATGGTTCTCGACCGCCGGATTGGAAGTCTGGAACATCGCCATTTTTATGATTTCCCGGATTTCGTCCGGAAAG
GCGATGTCCTGGTCATCAATGACTCCAAAGTCATTCCGGCCCGTCTTTCCGGCAGAAAAGAGACGGGAAGCCGCATCGAA
CTCCTCCTGCTGTCCCGATACTCCGATTCTCCAGCGGTTCCCGAAACCGATTCCACGGAATGGCCTTTCTTTCGGGTGGG
AGTTGAAGAAACATGGGAAGCCCTGCTCAAACCCGGGAAGCGGATCCGGATCGGGACAAGCATTTTTTTTGATGATCACA
GCCGGGCCACCGTGATCGAAAGGATCAATGAGAAAAAGTGGCTGTTGACCTTTAACACAGCCCTTCCGTTCGATCTTTTT
CTCCAGCGGTACGGCAAGGCGCCGCTTCCACCTTATATAAAACGGGAAAAGAAAAACGCACAAATGCCGGAGGATCGAGA
CCGATATCAGACCATTTACGCCCGGTCGCCGGGTTCCATTGCCGCTCCGACGGCGGGGTTTCACTTCTCGGAGGCATTGT
TCAAAACTCTGCGGGAACTTGAAATCGTCATCGCTCCCGTAACCCTGCATGTCGGCTTCGGCACCTTTACCCCGATCGAA
ACAGAGGACGTTGAAGATCACGTCATGGAGGTCGAATCTTTTTCAATTTCTCCGGAGTCATCGGATAAGATCAATTCCGC
CGAGCGGGTCATCGCCGTCGGAACGACCTCTACCCGGGTCCTGGAATCGGCCGCGGATGAGCAGGGTCGGGTTCACCCCA
TGTCCGGCCAGTCCAGGCTCTTCATCTATCCCGGATACCGTTTCAAAAGGGTACAGGCCCTCCTGACGAACTTCCATCTT
CCCAAATCCTCTCTTTTCCTGCTGGCCTGCGCCTTTGCCGGCAAAGACCGGATCCAGCAGGCTTATGCGACGGCCATTCA
GGAAGAATACCGCTTTTACAGTTACGGCGACTGCATGCTGATCATTTAG

Upstream 100 bases:

>100_bases
TTTACACATCATTTCTGATTATCAGGACCGCAACGCCGGCGGCATGTCCCGGAACAGCGGGCTGACCGTTTGAATTAAAG
GAAATGAACAGGACCCCTCA

Downstream 100 bases:

>100_bases
GCGGGCACGTTCCGAAAAGGCTTATCCGGATAAACTTATGCCTCCTTTAAAACAACATTTCAGGATACTGAATACTGACC
CGGGATCGGCGGCACGGCTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MMKLQDFDYQLPQSRIAQSPCAKRDHARMMVLDRRIGSLEHRHFYDFPDFVRKGDVLVINDSKVIPARLSGRKETGSRIE
LLLLSRYSDSPAVPETDSTEWPFFRVGVEETWEALLKPGKRIRIGTSIFFDDHSRATVIERINEKKWLLTFNTALPFDLF
LQRYGKAPLPPYIKREKKNAQMPEDRDRYQTIYARSPGSIAAPTAGFHFSEALFKTLRELEIVIAPVTLHVGFGTFTPIE
TEDVEDHVMEVESFSISPESSDKINSAERVIAVGTTSTRVLESAADEQGRVHPMSGQSRLFIYPGYRFKRVQALLTNFHL
PKSSLFLLACAFAGKDRIQQAYATAIQEEYRFYSYGDCMLII

Sequences:

>Translated_362_residues
MMKLQDFDYQLPQSRIAQSPCAKRDHARMMVLDRRIGSLEHRHFYDFPDFVRKGDVLVINDSKVIPARLSGRKETGSRIE
LLLLSRYSDSPAVPETDSTEWPFFRVGVEETWEALLKPGKRIRIGTSIFFDDHSRATVIERINEKKWLLTFNTALPFDLF
LQRYGKAPLPPYIKREKKNAQMPEDRDRYQTIYARSPGSIAAPTAGFHFSEALFKTLRELEIVIAPVTLHVGFGTFTPIE
TEDVEDHVMEVESFSISPESSDKINSAERVIAVGTTSTRVLESAADEQGRVHPMSGQSRLFIYPGYRFKRVQALLTNFHL
PKSSLFLLACAFAGKDRIQQAYATAIQEEYRFYSYGDCMLII
>Mature_362_residues
MMKLQDFDYQLPQSRIAQSPCAKRDHARMMVLDRRIGSLEHRHFYDFPDFVRKGDVLVINDSKVIPARLSGRKETGSRIE
LLLLSRYSDSPAVPETDSTEWPFFRVGVEETWEALLKPGKRIRIGTSIFFDDHSRATVIERINEKKWLLTFNTALPFDLF
LQRYGKAPLPPYIKREKKNAQMPEDRDRYQTIYARSPGSIAAPTAGFHFSEALFKTLRELEIVIAPVTLHVGFGTFTPIE
TEDVEDHVMEVESFSISPESSDKINSAERVIAVGTTSTRVLESAADEQGRVHPMSGQSRLFIYPGYRFKRVQALLTNFHL
PKSSLFLLACAFAGKDRIQQAYATAIQEEYRFYSYGDCMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=366, Percent_Identity=40.1639344262295, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SYNAS (Q2LVG6)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462248.1
- HSSP:   O32054
- ProteinModelPortal:   Q2LVG6
- SMR:   Q2LVG6
- STRING:   Q2LVG6
- GeneID:   3884155
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00039
- NMPDR:   fig|56780.10.peg.2182
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- PhylomeDB:   Q2LVG6
- BioCyc:   SACI56780:SYN_00039-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41446; Mature: 41446

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLQDFDYQLPQSRIAQSPCAKRDHARMMVLDRRIGSLEHRHFYDFPDFVRKGDVLVIN
CCCCCCCCCCCCHHHHHCCCCCCCCCHHEEEEHHHHCCCCCCCCCCCHHHHHCCCEEEEE
DSKVIPARLSGRKETGSRIELLLLSRYSDSPAVPETDSTEWPFFRVGVEETWEALLKPGK
CCEEEEHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCC
RIRIGTSIFFDDHSRATVIERINEKKWLLTFNTALPFDLFLQRYGKAPLPPYIKREKKNA
EEEEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHCCCCCC
QMPEDRDRYQTIYARSPGSIAAPTAGFHFSEALFKTLRELEIVIAPVTLHVGFGTFTPIE
CCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
TEDVEDHVMEVESFSISPESSDKINSAERVIAVGTTSTRVLESAADEQGRVHPMSGQSRL
CCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEE
FIYPGYRFKRVQALLTNFHLPKSSLFLLACAFAGKDRIQQAYATAIQEEYRFYSYGDCML
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCEEE
II
EC
>Mature Secondary Structure
MMKLQDFDYQLPQSRIAQSPCAKRDHARMMVLDRRIGSLEHRHFYDFPDFVRKGDVLVIN
CCCCCCCCCCCCHHHHHCCCCCCCCCHHEEEEHHHHCCCCCCCCCCCHHHHHCCCEEEEE
DSKVIPARLSGRKETGSRIELLLLSRYSDSPAVPETDSTEWPFFRVGVEETWEALLKPGK
CCEEEEHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCC
RIRIGTSIFFDDHSRATVIERINEKKWLLTFNTALPFDLFLQRYGKAPLPPYIKREKKNA
EEEEEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHCCCCCC
QMPEDRDRYQTIYARSPGSIAAPTAGFHFSEALFKTLRELEIVIAPVTLHVGFGTFTPIE
CCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
TEDVEDHVMEVESFSISPESSDKINSAERVIAVGTTSTRVLESAADEQGRVHPMSGQSRL
CCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEE
FIYPGYRFKRVQALLTNFHLPKSSLFLLACAFAGKDRIQQAYATAIQEEYRFYSYGDCML
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCEEE
II
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA