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The map label for this gene is ilvE [H]

Identifier: 85858680

GI number: 85858680

Start: 857974

End: 858660

Strand: Direct

Name: ilvE [H]

Synonym: SYN_03154

Alternate gene names: 85858680

Gene position: 857974-858660 (Clockwise)

Preceding gene: 85858679

Following gene: 85858681

Centisome position: 26.99

GC content: 49.05

Gene sequence:

>687_bases
ATGTTTAACCACGAAAGAGTTCGTAATCATGAAGGAGACGCACTTATGGAGATTAAAATGAATCCGCTGTCGCCGGAATC
CAGAAAAACAAAGCCGGCAGATGAATCGAAATTGGGATTCGGCAAATTTTTCACGGATCATATGTTTACGATGACCTATC
AAACCGATCGCGGATGGGAAAATGCGGAGATCGGCCCGTATCGGCCCATCTGTCTCGATCCGACGGCCATGTGTCTTCAT
TACGGTCAGGAAATATTCGAAGGGATGAAGGCCTATCGCGGACGGTCAGGTGAAATCTATCTTTTCCGGCCCAAGGAAAA
TATTCGACGCATGAATGCGTCTGCTGAGCGACTCTGCATGCCTGCCCTTGACCCGGATGTATTTATGGAAGCTCTGGTCA
AGCTGGTTCTTCTGGAAAAAGACTGGATACCCCATGGGCGGGGTACATCCCTGTATATCAGACCAACCATGATTGCTACC
GAGGCAGCGCTGGGCGTACATCCATCTGATGAATATCTCTTTTTCATTATCGTCGGCCCGGTAGGAGCCTATTATCCTGA
AGGATTCCAATCCGACGAAAATTTTACGTCAGCGAGGAATATGTTCGATCAGTGCGTGGCGGGACAGGGACCTGCAAGAA
CGCGGGGAATTATGCGGCAAGTCTTTATGCCAGTCAGATTGCCATGA

Upstream 100 bases:

>100_bases
CGGCCACACCTATACACATGACGTGGGTAGTGGGGCACTTTTCCGGAATTCACTCTGTCAGGGGCAAGGACGATTCAGAA
GGAAAGCTTCGGCATAAGAA

Downstream 100 bases:

>100_bases
AAATGGGCTACACCCAGGGTTTTATGGCTGGATGCCATCGAGAGAAAGTACGTCGAAGAGGTGGGAACCAGCAATATCTT
TTTCCTGATTGGCGATGAAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 1 [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MFNHERVRNHEGDALMEIKMNPLSPESRKTKPADESKLGFGKFFTDHMFTMTYQTDRGWENAEIGPYRPICLDPTAMCLH
YGQEIFEGMKAYRGRSGEIYLFRPKENIRRMNASAERLCMPALDPDVFMEALVKLVLLEKDWIPHGRGTSLYIRPTMIAT
EAALGVHPSDEYLFFIIVGPVGAYYPEGFQSDENFTSARNMFDQCVAGQGPARTRGIMRQVFMPVRLP

Sequences:

>Translated_228_residues
MFNHERVRNHEGDALMEIKMNPLSPESRKTKPADESKLGFGKFFTDHMFTMTYQTDRGWENAEIGPYRPICLDPTAMCLH
YGQEIFEGMKAYRGRSGEIYLFRPKENIRRMNASAERLCMPALDPDVFMEALVKLVLLEKDWIPHGRGTSLYIRPTMIAT
EAALGVHPSDEYLFFIIVGPVGAYYPEGFQSDENFTSARNMFDQCVAGQGPARTRGIMRQVFMPVRLP
>Mature_228_residues
MFNHERVRNHEGDALMEIKMNPLSPESRKTKPADESKLGFGKFFTDHMFTMTYQTDRGWENAEIGPYRPICLDPTAMCLH
YGQEIFEGMKAYRGRSGEIYLFRPKENIRRMNASAERLCMPALDPDVFMEALVKLVLLEKDWIPHGRGTSLYIRPTMIAT
EAALGVHPSDEYLFFIIVGPVGAYYPEGFQSDENFTSARNMFDQCVAGQGPARTRGIMRQVFMPVRLP

Specific function: Transaminates branched-chain amino acids and ketoglutarate. Involved in the final step of the methionine regeneration pathway, where ketomethiobutyrate (KMTB) is converted to methionine via a transamination. The amino donor preference is isoleucine, leuci

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=186, Percent_Identity=39.7849462365591, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI296010904, Length=160, Percent_Identity=43.125, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI38176287, Length=160, Percent_Identity=43.125, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI296010906, Length=160, Percent_Identity=43.125, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI258614015, Length=133, Percent_Identity=39.8496240601504, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI296010902, Length=173, Percent_Identity=30.0578034682081, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI296010900, Length=160, Percent_Identity=32.5, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI48994963, Length=136, Percent_Identity=30.8823529411765, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17568601, Length=183, Percent_Identity=40.4371584699454, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17565728, Length=154, Percent_Identity=40.2597402597403, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322608, Length=169, Percent_Identity=46.1538461538462, Blast_Score=172, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322002, Length=176, Percent_Identity=42.6136363636364, Blast_Score=155, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24641779, Length=188, Percent_Identity=40.4255319148936, Blast_Score=161, Evalue=3e-40,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 26099; Mature: 26099

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNHERVRNHEGDALMEIKMNPLSPESRKTKPADESKLGFGKFFTDHMFTMTYQTDRGWE
CCCCHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCC
NAEIGPYRPICLDPTAMCLHYGQEIFEGMKAYRGRSGEIYLFRPKENIRRMNASAERLCM
CCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCHHHHCC
PALDPDVFMEALVKLVLLEKDWIPHGRGTSLYIRPTMIATEAALGVHPSDEYLFFIIVGP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHEEHHHHHCCCCCCCEEEEEEECC
VGAYYPEGFQSDENFTSARNMFDQCVAGQGPARTRGIMRQVFMPVRLP
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFNHERVRNHEGDALMEIKMNPLSPESRKTKPADESKLGFGKFFTDHMFTMTYQTDRGWE
CCCCHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCC
NAEIGPYRPICLDPTAMCLHYGQEIFEGMKAYRGRSGEIYLFRPKENIRRMNASAERLCM
CCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCHHHHCC
PALDPDVFMEALVKLVLLEKDWIPHGRGTSLYIRPTMIATEAALGVHPSDEYLFFIIVGP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHEEHHHHHCCCCCCCEEEEEEECC
VGAYYPEGFQSDENFTSARNMFDQCVAGQGPARTRGIMRQVFMPVRLP
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]