Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 85858605

GI number: 85858605

Start: 771519

End: 772946

Strand: Direct

Name: pyk [H]

Synonym: SYN_02952

Alternate gene names: 85858605

Gene position: 771519-772946 (Clockwise)

Preceding gene: 85858604

Following gene: 85858609

Centisome position: 24.27

GC content: 57.91

Gene sequence:

>1428_bases
ATGAAGATTTCTTCACCGAAGACGAAGATCGTCTGCACCATCGGGCCGGCGTCTGAGAATCCCGACGTGATGAAACAGAT
GATCGAAGCGGGAATGAACGTCGCCCGACTCAACTTCTCCCATGGCGACTTCGCGTCCCACGGGAAAGTCATCGGGAATC
TGCGGGCTGTGTCGGCATTGCTCGGCCGGAGGATCGCAATCCTGGCCGATCTTTCCGGTCCGAAGATGCGTATCGGAACG
TTTGCATCCGAACCGATCCAACTCGATACGGGTGACCTGTTCACGCTGACGACCGAGGACATCGTCGGTGACCGGAATCG
AGTGTCCGTCTCATTCGATCGCCTTCCGAAGTCTGTAAGACCCGGGGACATGCTTTTTCTCAATGACGGATACATTCAGC
TCAAGGTGAACGAGGCCAGGGGCAATGATGTCTTATGCAGCGTCATGGTCGGCGGCGAACTTCGTTCCCGCAAGGGACTC
AATCTGCCGGGCATCGATCTCGGGATCAGCGCTTTTACCGAACGTGATCGTGAGTGCCTGCAGTTTGCCCTCGAGAACGG
GGTGGACGCCGTGAGCCAGTCCTTCGTCGAAGGACCGGAGGATGTCGAGGCCGTGCGCAGGGCCGCCGCAGACATGGGAT
ACACTCCCTTCGTTATCGCGAAGATCGAACGGTCCGGGGCGCTTGATCGCATTGAGGAGATCATCGCCGCAGCCGACGGC
ATCATGATCGCCCGCGGCGACCTGGGTGTTGAAGTTCCGATCGAACAGATCGCCATGGTCCAGAAGCGGGTTATGCTCCT
TGCAAACATGTGCGCGAAGCCCGTCATTACGGCAACACAGATGCTCGAGTCGATGACGGAAAATCGACGCCCTACCCGCG
CCGAGGCAACCGATGTGGCCAACGCGATTCTTGACGGCACCGACTGCGTCATGCTTTCAGGCGAATCCGCCATGGGGAGG
TATCCTGTGGAAGCTGTCGGGATGCTGGCGAAAATAGCCCTTTCGGCGGAAGCGCACCGCTCCATGATCGCGGTCAAAGA
GATGTTCAAGGGAATCGATCTTCGAGGGAGGGTTCGTCTGGAACATCTCATTGCGATCGGCGTAGAGGCGTGCTTCGAAT
TTGTGACACCGGCGGCCGTGTTTGTCCCGACGCGAAGCGGCGCCACGGCGCGGAGCATGGCCAGATTCCGCTTTCCCGTA
TGGACTGTGGCCGTGAGTTCCGAAGAATCGACCTGCCAGGAACTCCTTTTCTCCTCAGGGGTTTATCCGGTGCGCGAGCT
TCCCATGCCTGAGAACTGGAATGATTATGTCAAAAGGCAAGTCGCTGATCTTGGTCTGGAAGGAAAAATCGCAATTCTGA
CCCGGGGTCCGTCGCGGGAGCATTCCGAGACGAATCACGGCGTGGAAATTCTCGACCTGACCATTTGA

Upstream 100 bases:

>100_bases
CGATATGATGTAAAGGTGAAACCTGGATGAAAGTGGAATCCGTCGAGGAAGACGCGAAAGCGGGCTATGCCGGGCGGCTC
AATGGACAGGGGGAAAAGGC

Downstream 100 bases:

>100_bases
CAGTGGAAACGATGAGGCGCAGACGGGGCAAGTGAGGAGGAAAAAATGAAATTGTTGTAAGGCTGTCCGGGGATTTTCGG
GAATGAACCGGTCAGGATGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKISSPKTKIVCTIGPASENPDVMKQMIEAGMNVARLNFSHGDFASHGKVIGNLRAVSALLGRRIAILADLSGPKMRIGT
FASEPIQLDTGDLFTLTTEDIVGDRNRVSVSFDRLPKSVRPGDMLFLNDGYIQLKVNEARGNDVLCSVMVGGELRSRKGL
NLPGIDLGISAFTERDRECLQFALENGVDAVSQSFVEGPEDVEAVRRAAADMGYTPFVIAKIERSGALDRIEEIIAAADG
IMIARGDLGVEVPIEQIAMVQKRVMLLANMCAKPVITATQMLESMTENRRPTRAEATDVANAILDGTDCVMLSGESAMGR
YPVEAVGMLAKIALSAEAHRSMIAVKEMFKGIDLRGRVRLEHLIAIGVEACFEFVTPAAVFVPTRSGATARSMARFRFPV
WTVAVSSEESTCQELLFSSGVYPVRELPMPENWNDYVKRQVADLGLEGKIAILTRGPSREHSETNHGVEILDLTI

Sequences:

>Translated_475_residues
MKISSPKTKIVCTIGPASENPDVMKQMIEAGMNVARLNFSHGDFASHGKVIGNLRAVSALLGRRIAILADLSGPKMRIGT
FASEPIQLDTGDLFTLTTEDIVGDRNRVSVSFDRLPKSVRPGDMLFLNDGYIQLKVNEARGNDVLCSVMVGGELRSRKGL
NLPGIDLGISAFTERDRECLQFALENGVDAVSQSFVEGPEDVEAVRRAAADMGYTPFVIAKIERSGALDRIEEIIAAADG
IMIARGDLGVEVPIEQIAMVQKRVMLLANMCAKPVITATQMLESMTENRRPTRAEATDVANAILDGTDCVMLSGESAMGR
YPVEAVGMLAKIALSAEAHRSMIAVKEMFKGIDLRGRVRLEHLIAIGVEACFEFVTPAAVFVPTRSGATARSMARFRFPV
WTVAVSSEESTCQELLFSSGVYPVRELPMPENWNDYVKRQVADLGLEGKIAILTRGPSREHSETNHGVEILDLTI
>Mature_475_residues
MKISSPKTKIVCTIGPASENPDVMKQMIEAGMNVARLNFSHGDFASHGKVIGNLRAVSALLGRRIAILADLSGPKMRIGT
FASEPIQLDTGDLFTLTTEDIVGDRNRVSVSFDRLPKSVRPGDMLFLNDGYIQLKVNEARGNDVLCSVMVGGELRSRKGL
NLPGIDLGISAFTERDRECLQFALENGVDAVSQSFVEGPEDVEAVRRAAADMGYTPFVIAKIERSGALDRIEEIIAAADG
IMIARGDLGVEVPIEQIAMVQKRVMLLANMCAKPVITATQMLESMTENRRPTRAEATDVANAILDGTDCVMLSGESAMGR
YPVEAVGMLAKIALSAEAHRSMIAVKEMFKGIDLRGRVRLEHLIAIGVEACFEFVTPAAVFVPTRSGATARSMARFRFPV
WTVAVSSEESTCQELLFSSGVYPVRELPMPENWNDYVKRQVADLGLEGKIAILTRGPSREHSETNHGVEILDLTI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=457, Percent_Identity=38.9496717724289, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI33286420, Length=457, Percent_Identity=38.9496717724289, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI33286418, Length=452, Percent_Identity=38.4955752212389, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI10835121, Length=467, Percent_Identity=40.0428265524625, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI32967597, Length=467, Percent_Identity=40.0428265524625, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=37.2413793103448, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=37.2413793103448, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=34.5833333333333, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=34.5833333333333, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI310128738, Length=209, Percent_Identity=33.9712918660287, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=40.2569593147752, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788160, Length=432, Percent_Identity=39.5833333333333, Blast_Score=282, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=424, Percent_Identity=38.9150943396226, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71984413, Length=452, Percent_Identity=38.0530973451327, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=452, Percent_Identity=38.0530973451327, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=452, Percent_Identity=38.0530973451327, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=430, Percent_Identity=39.3023255813954, Blast_Score=275, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=461, Percent_Identity=36.2255965292842, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324923, Length=458, Percent_Identity=34.4978165938865, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24648964, Length=440, Percent_Identity=38.1818181818182, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI28571814, Length=440, Percent_Identity=38.1818181818182, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=38.5922330097087, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24581235, Length=438, Percent_Identity=33.1050228310502, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24646914, Length=309, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51626; Mature: 51626

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISSPKTKIVCTIGPASENPDVMKQMIEAGMNVARLNFSHGDFASHGKVIGNLRAVSAL
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
LGRRIAILADLSGPKMRIGTFASEPIQLDTGDLFTLTTEDIVGDRNRVSVSFDRLPKSVR
HCCEEEEEEECCCCCEEEECCCCCCEEECCCCEEEEEHHHHCCCCCEEEEEHHHCCCCCC
PGDMLFLNDGYIQLKVNEARGNDVLCSVMVGGELRSRKGLNLPGIDLGISAFTERDRECL
CCCEEEEECCEEEEEEECCCCCCEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHH
QFALENGVDAVSQSFVEGPEDVEAVRRAAADMGYTPFVIAKIERSGALDRIEEIIAAADG
HHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
IMIARGDLGVEVPIEQIAMVQKRVMLLANMCAKPVITATQMLESMTENRRPTRAEATDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
NAILDGTDCVMLSGESAMGRYPVEAVGMLAKIALSAEAHRSMIAVKEMFKGIDLRGRVRL
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EHLIAIGVEACFEFVTPAAVFVPTRSGATARSMARFRFPVWTVAVSSEESTCQELLFSSG
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC
VYPVRELPMPENWNDYVKRQVADLGLEGKIAILTRGPSREHSETNHGVEILDLTI
CCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MKISSPKTKIVCTIGPASENPDVMKQMIEAGMNVARLNFSHGDFASHGKVIGNLRAVSAL
CCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
LGRRIAILADLSGPKMRIGTFASEPIQLDTGDLFTLTTEDIVGDRNRVSVSFDRLPKSVR
HCCEEEEEEECCCCCEEEECCCCCCEEECCCCEEEEEHHHHCCCCCEEEEEHHHCCCCCC
PGDMLFLNDGYIQLKVNEARGNDVLCSVMVGGELRSRKGLNLPGIDLGISAFTERDRECL
CCCEEEEECCEEEEEEECCCCCCEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHH
QFALENGVDAVSQSFVEGPEDVEAVRRAAADMGYTPFVIAKIERSGALDRIEEIIAAADG
HHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCC
IMIARGDLGVEVPIEQIAMVQKRVMLLANMCAKPVITATQMLESMTENRRPTRAEATDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
NAILDGTDCVMLSGESAMGRYPVEAVGMLAKIALSAEAHRSMIAVKEMFKGIDLRGRVRL
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EHLIAIGVEACFEFVTPAAVFVPTRSGATARSMARFRFPVWTVAVSSEESTCQELLFSSG
HHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCC
VYPVRELPMPENWNDYVKRQVADLGLEGKIAILTRGPSREHSETNHGVEILDLTI
CCCHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]