Click here to switch to the map view.

The map label for this gene is acrA [H]

Identifier: 85858609

GI number: 85858609

Start: 776332

End: 777702

Strand: Direct

Name: acrA [H]

Synonym: SYN_02948

Alternate gene names: 85858609

Gene position: 776332-777702 (Clockwise)

Preceding gene: 85858605

Following gene: 85858610

Centisome position: 24.42

GC content: 56.16

Gene sequence:

>1371_bases
ATGTACCGGAAACAATCCCTGCCGGAGGGAAAAATCAAGGATCGGACTGAAGAATGTGTGAACCGCCTTAATAGAGGCTT
TGGACAAAACATGGGGAGGAGATCGGACATGTGGAGCATTTCGGCACAGCGTCATCCAGCTCGCCATTTGTGGAGTTTAT
TGCGGATCATTTCATACTTTGCAATCGTCATCCTGTTTCTCGCGCCGGTGGCGGGCTGCTCGAAAGGTTCGAAGGAGGCG
CCCAAACCGCCGGTTGTGGAGGTGACGACCGTCATCCAGAAGGACGTTCCCGTTTACAGAGAGTGGATCGGGACGCTCGA
CGGGATGGTGAATGCCACAATCCGGGCGCAGGTTCAGGGGTATCTCGTTAAACAGAACTATCGCGACGGCGACGTCGTAA
AAAAGGGGCAGGTCCTCTTTGAAATCGACCCCCGCACCTTTCAGACAGCCCTGGGGCAGGCAAGGGGACAATTGGAAGCG
CAGCAGGCGCGCTGGAATACGGCCAGGGCAAATATGGTCCGGATCAAGCCTCTTGCCGATGCCAATGCCGTCAGCAAAAA
AGATCTCGATGACGCAATCGGAGCGGAACAGGCTGCCCGGGCGGCGGTGCATTCCGCCCAGGCCAGTGTCGACAGGGCCC
AACTGGAACTGGGTTTCACAAAGATCGTCTCCCCGGTGGAGGGAATCGCCGGTCTTGCCAAAGCCCAGATCGGCAATCTC
GTGGGGCCGGGGGCAATGGAAGAGCTGACGACGGTCTCCACGGTCAATCCGATCAAAGTCTACTTCCAGATAAGCGAGCA
GGAATACATCCAGACCGCTCAACGTTCCGCTGCCTCGAGGGGGATAAGGGACGGTGAGCTGATTCTTGCCGATGGAAGCA
TCTATCCTCAAAAGGGTTATTTCGCCTTTGCCGATCGGCAGGTGGATATCACGACCGGGACCATTAAAGTTGCAGCCATC
TTTCCCAACCCGGACAACTTTCTGCGGCCCGGCCAGTATGGAAGAGTGCGTATCGCCGTGCATGTCCGAAAGAATGCCCT
TCTCGTGCCCCAGCGGGCCGTGTCTGAAGTCCAGGGGAAATACATGGCGGCCGTGGTGGGATCGGATAACAGGGTGGAAA
TCCGGCCTGTAAAAACCGCGGAGAGGATCGACAACCTGTGGGTTATCCTTGAAGGCCTCAAACCCGGAGAGCGCGTGATT
GTCGAGGGGATTCAGAAGGTTCGCCCCGGAATTCCGGTGATGCCAACGGCCTTTGTCGAAGCATCCTCAAAGCCGGAAGC
GAAGACAGAAGCAGTTCCTGAAAGGAAAGCGGAAGAAAAATTCCAGTCTCCCTCAAAACCAGCAGATCCTGCAAAGACTG
AAAAGAGGTGA

Upstream 100 bases:

>100_bases
AACATCTTTTTCAGGCGATAATAATTAACAGATATCAAATAGTTGCAGCTTGCCTGGCAATAATCCGCTTCAGGTATGTT
TTTTGGACTTTATTTCGTAG

Downstream 100 bases:

>100_bases
TCCGCCATGTCCAGGTTCTTCATCAACCGCCCCATCGTGGCCATGGTGATCGCCATCCTTATGGTCATTGTCGGCCTCGT
GGCCATGTCGGGTCTGCCGA

Product: acriflavin resistance periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MYRKQSLPEGKIKDRTEECVNRLNRGFGQNMGRRSDMWSISAQRHPARHLWSLLRIISYFAIVILFLAPVAGCSKGSKEA
PKPPVVEVTTVIQKDVPVYREWIGTLDGMVNATIRAQVQGYLVKQNYRDGDVVKKGQVLFEIDPRTFQTALGQARGQLEA
QQARWNTARANMVRIKPLADANAVSKKDLDDAIGAEQAARAAVHSAQASVDRAQLELGFTKIVSPVEGIAGLAKAQIGNL
VGPGAMEELTTVSTVNPIKVYFQISEQEYIQTAQRSAASRGIRDGELILADGSIYPQKGYFAFADRQVDITTGTIKVAAI
FPNPDNFLRPGQYGRVRIAVHVRKNALLVPQRAVSEVQGKYMAAVVGSDNRVEIRPVKTAERIDNLWVILEGLKPGERVI
VEGIQKVRPGIPVMPTAFVEASSKPEAKTEAVPERKAEEKFQSPSKPADPAKTEKR

Sequences:

>Translated_456_residues
MYRKQSLPEGKIKDRTEECVNRLNRGFGQNMGRRSDMWSISAQRHPARHLWSLLRIISYFAIVILFLAPVAGCSKGSKEA
PKPPVVEVTTVIQKDVPVYREWIGTLDGMVNATIRAQVQGYLVKQNYRDGDVVKKGQVLFEIDPRTFQTALGQARGQLEA
QQARWNTARANMVRIKPLADANAVSKKDLDDAIGAEQAARAAVHSAQASVDRAQLELGFTKIVSPVEGIAGLAKAQIGNL
VGPGAMEELTTVSTVNPIKVYFQISEQEYIQTAQRSAASRGIRDGELILADGSIYPQKGYFAFADRQVDITTGTIKVAAI
FPNPDNFLRPGQYGRVRIAVHVRKNALLVPQRAVSEVQGKYMAAVVGSDNRVEIRPVKTAERIDNLWVILEGLKPGERVI
VEGIQKVRPGIPVMPTAFVEASSKPEAKTEAVPERKAEEKFQSPSKPADPAKTEKR
>Mature_456_residues
MYRKQSLPEGKIKDRTEECVNRLNRGFGQNMGRRSDMWSISAQRHPARHLWSLLRIISYFAIVILFLAPVAGCSKGSKEA
PKPPVVEVTTVIQKDVPVYREWIGTLDGMVNATIRAQVQGYLVKQNYRDGDVVKKGQVLFEIDPRTFQTALGQARGQLEA
QQARWNTARANMVRIKPLADANAVSKKDLDDAIGAEQAARAAVHSAQASVDRAQLELGFTKIVSPVEGIAGLAKAQIGNL
VGPGAMEELTTVSTVNPIKVYFQISEQEYIQTAQRSAASRGIRDGELILADGSIYPQKGYFAFADRQVDITTGTIKVAAI
FPNPDNFLRPGQYGRVRIAVHVRKNALLVPQRAVSEVQGKYMAAVVGSDNRVEIRPVKTAERIDNLWVILEGLKPGERVI
VEGIQKVRPGIPVMPTAFVEASSKPEAKTEAVPERKAEEKFQSPSKPADPAKTEKR

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=391, Percent_Identity=35.0383631713555, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789929, Length=381, Percent_Identity=34.6456692913386, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=34.6153846153846, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87082036, Length=376, Percent_Identity=30.3191489361702, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87081796, Length=341, Percent_Identity=26.6862170087977, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081951, Length=143, Percent_Identity=33.5664335664336, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 50199; Mature: 50199

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKQSLPEGKIKDRTEECVNRLNRGFGQNMGRRSDMWSISAQRHPARHLWSLLRIISYF
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
AIVILFLAPVAGCSKGSKEAPKPPVVEVTTVIQKDVPVYREWIGTLDGMVNATIRAQVQG
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEE
YLVKQNYRDGDVVKKGQVLFEIDPRTFQTALGQARGQLEAQQARWNTARANMVRIKPLAD
EEEECCCCCCCCEECCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCHHHCCEEEEEECCC
ANAVSKKDLDDAIGAEQAARAAVHSAQASVDRAQLELGFTKIVSPVEGIAGLAKAQIGNL
CCCCCHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGPGAMEELTTVSTVNPIKVYFQISEQEYIQTAQRSAASRGIRDGELILADGSIYPQKGY
CCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCE
FAFADRQVDITTGTIKVAAIFPNPDNFLRPGQYGRVRIAVHVRKNALLVPQRAVSEVQGK
EEEECCEEEEEECEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCEEECCHHHHHHHCCC
YMAAVVGSDNRVEIRPVKTAERIDNLWVILEGLKPGERVIVEGIQKVRPGIPVMPTAFVE
EEEEEECCCCEEEEEECCHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHC
ASSKPEAKTEAVPERKAEEKFQSPSKPADPAKTEKR
CCCCCCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYRKQSLPEGKIKDRTEECVNRLNRGFGQNMGRRSDMWSISAQRHPARHLWSLLRIISYF
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
AIVILFLAPVAGCSKGSKEAPKPPVVEVTTVIQKDVPVYREWIGTLDGMVNATIRAQVQG
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEE
YLVKQNYRDGDVVKKGQVLFEIDPRTFQTALGQARGQLEAQQARWNTARANMVRIKPLAD
EEEECCCCCCCCEECCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCHHHCCEEEEEECCC
ANAVSKKDLDDAIGAEQAARAAVHSAQASVDRAQLELGFTKIVSPVEGIAGLAKAQIGNL
CCCCCHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGPGAMEELTTVSTVNPIKVYFQISEQEYIQTAQRSAASRGIRDGELILADGSIYPQKGY
CCCCHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCE
FAFADRQVDITTGTIKVAAIFPNPDNFLRPGQYGRVRIAVHVRKNALLVPQRAVSEVQGK
EEEECCEEEEEECEEEEEEECCCCCCCCCCCCCCEEEEEEEEECCEEECCHHHHHHHCCC
YMAAVVGSDNRVEIRPVKTAERIDNLWVILEGLKPGERVIVEGIQKVRPGIPVMPTAFVE
EEEEEECCCCEEEEEECCHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHC
ASSKPEAKTEAVPERKAEEKFQSPSKPADPAKTEKR
CCCCCCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]