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The map label for this gene is pfp [H]

Identifier: 85858604

GI number: 85858604

Start: 770127

End: 771431

Strand: Direct

Name: pfp [H]

Synonym: SYN_02953

Alternate gene names: 85858604

Gene position: 770127-771431 (Clockwise)

Preceding gene: 85858603

Following gene: 85858605

Centisome position: 24.22

GC content: 57.16

Gene sequence:

>1305_bases
ATGAGACAGGAGGGTGACATGGGGAATACGGAGCTTGATTTCACGATTGAACGGCTTGGCGAGTGCCATATCCCTTCCCC
GGTGAAGGGGATCCGCTTTGTGGATGATGATGGGCGTGTCCTTTATCACTCTCATCTCCAGGAGATGAAGCCATGGCTTG
ACGAAGGGATTCATCCGCCCGCGATGGAAGCGGCGGGGCCAAGGGAAAAAGTCTTTTTTGATCCGTCGCGGATCGCCTGC
GGCATCGTGACCTGCGGCGGGATCTGTCCCGGACTGAACGACGTGATCCGATCAATCGTTCTGAGTCTGTGTCACCATTA
CTGCGTGCAGAAGATATACGGGTTCCGTTTCGGTTACGAGGGGCTCGTGCAGCGGTATGGACATAAGCCACTGGAGCTGA
CAGCGGATTCGGTTGCCCAGATCCACGAGATCGGCGGGACGATTCTGGGCTCGTCCCGGGGACCGCAGGAACCCTCCGAG
ATGGTGAAAACGCTTGAGGATCTCAATGTCGGCATTCTCTTTACCATCGGGGGTGACGGGACGCAGCGCGGGGCTCAGAA
AATCGCCGAAGAAGCGACCCGGCGGGGGCTCGAAATCAGTGTCATCGGCATTCCAAAAACGATCGACAATGATATTTCCT
ATATCCAGGCTACCTTCGGATTTGAAACGGCGGTTGCCGAGGCCCGTCGGGCCACCTACGCCGCCCACACCGAGGCGGAT
GCGGCGCGCAACAGCATCAGCCTGGTGAAGCTCATGGGCCGTGACTCCGGGTTCATTGCCGCGTATTCTGTCCTCGTGAA
CAGCCATGTGAACTTTTGTCTGGTTCCGGAAGCCCGCTTCACCCTGGATGGCCTTCTCCGCGCCCTGAAAGAGCGCCTCG
AGCGCAGGGGACACGCGGTGGTCGTCGTGGCCGAGGGGGCTGGTCAAAACCTGATGGAGGCGACAGCTGAACGCGATGCC
TCGGGAAATATCAAGTATGGCGACATCGGCATATTTCTGCGTGATGCCATCAGGGATTATTTCAGGAAAGCCAGCCTGGA
AATTAATCTCAAATACATCGATCCCAGTTATATCATCCGGAGCATGCCGGCCAATCCGCATGACGCCGCCTTCTGCCTTC
TCCTCGGGCAAAGCGCCGTACATGCCGGAATGAGTGGACGAACGAACATGGTGGTCAGTTTCTGGAACCGTGAATTCACG
CATGTGCCCATAACGCTTGCCGTGTCGCAGCGCAAAAAGATCGATACGTCCAGCATGCTTTGGAACAGCGTCCTGGCGGT
GACCGGTCAACCCGATATGATGTAA

Upstream 100 bases:

>100_bases
ATGATCCGCGGGTTTATCTGGGCCTGGCCGAGGCCGCCATGGCACAGCGGGTCAGGCAGGCCGTGACCGAACTGCGAAGC
CGGAACACGACGCTGATGAA

Downstream 100 bases:

>100_bases
AGGTGAAACCTGGATGAAAGTGGAATCCGTCGAGGAAGACGCGAAAGCGGGCTATGCCGGGCGGCTCAATGGACAGGGGG
AAAAGGCATGAAGATTTCTT

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRQEGDMGNTELDFTIERLGECHIPSPVKGIRFVDDDGRVLYHSHLQEMKPWLDEGIHPPAMEAAGPREKVFFDPSRIAC
GIVTCGGICPGLNDVIRSIVLSLCHHYCVQKIYGFRFGYEGLVQRYGHKPLELTADSVAQIHEIGGTILGSSRGPQEPSE
MVKTLEDLNVGILFTIGGDGTQRGAQKIAEEATRRGLEISVIGIPKTIDNDISYIQATFGFETAVAEARRATYAAHTEAD
AARNSISLVKLMGRDSGFIAAYSVLVNSHVNFCLVPEARFTLDGLLRALKERLERRGHAVVVVAEGAGQNLMEATAERDA
SGNIKYGDIGIFLRDAIRDYFRKASLEINLKYIDPSYIIRSMPANPHDAAFCLLLGQSAVHAGMSGRTNMVVSFWNREFT
HVPITLAVSQRKKIDTSSMLWNSVLAVTGQPDMM

Sequences:

>Translated_434_residues
MRQEGDMGNTELDFTIERLGECHIPSPVKGIRFVDDDGRVLYHSHLQEMKPWLDEGIHPPAMEAAGPREKVFFDPSRIAC
GIVTCGGICPGLNDVIRSIVLSLCHHYCVQKIYGFRFGYEGLVQRYGHKPLELTADSVAQIHEIGGTILGSSRGPQEPSE
MVKTLEDLNVGILFTIGGDGTQRGAQKIAEEATRRGLEISVIGIPKTIDNDISYIQATFGFETAVAEARRATYAAHTEAD
AARNSISLVKLMGRDSGFIAAYSVLVNSHVNFCLVPEARFTLDGLLRALKERLERRGHAVVVVAEGAGQNLMEATAERDA
SGNIKYGDIGIFLRDAIRDYFRKASLEINLKYIDPSYIIRSMPANPHDAAFCLLLGQSAVHAGMSGRTNMVVSFWNREFT
HVPITLAVSQRKKIDTSSMLWNSVLAVTGQPDMM
>Mature_434_residues
MRQEGDMGNTELDFTIERLGECHIPSPVKGIRFVDDDGRVLYHSHLQEMKPWLDEGIHPPAMEAAGPREKVFFDPSRIAC
GIVTCGGICPGLNDVIRSIVLSLCHHYCVQKIYGFRFGYEGLVQRYGHKPLELTADSVAQIHEIGGTILGSSRGPQEPSE
MVKTLEDLNVGILFTIGGDGTQRGAQKIAEEATRRGLEISVIGIPKTIDNDISYIQATFGFETAVAEARRATYAAHTEAD
AARNSISLVKLMGRDSGFIAAYSVLVNSHVNFCLVPEARFTLDGLLRALKERLERRGHAVVVVAEGAGQNLMEATAERDA
SGNIKYGDIGIFLRDAIRDYFRKASLEINLKYIDPSYIIRSMPANPHDAAFCLLLGQSAVHAGMSGRTNMVVSFWNREFT
HVPITLAVSQRKKIDTSSMLWNSVLAVTGQPDMM

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=271, Percent_Identity=29.520295202952, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI11321601, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI266453619, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI266453768, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI266453748, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI4505749, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790350, Length=232, Percent_Identity=32.3275862068966, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25147584, Length=271, Percent_Identity=28.0442804428044, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558788, Length=233, Percent_Identity=27.4678111587983, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323861, Length=257, Percent_Identity=27.6264591439689, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321679, Length=329, Percent_Identity=23.7082066869301, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28573326, Length=252, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17647809, Length=252, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24652337, Length=252, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 47724; Mature: 47724

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQEGDMGNTELDFTIERLGECHIPSPVKGIRFVDDDGRVLYHSHLQEMKPWLDEGIHPP
CCCCCCCCCCCCEEEHHHCCCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCC
AMEAAGPREKVFFDPSRIACGIVTCGGICPGLNDVIRSIVLSLCHHYCVQKIYGFRFGYE
CCCCCCCCCCEEECCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLVQRYGHKPLELTADSVAQIHEIGGTILGSSRGPQEPSEMVKTLEDLNVGILFTIGGDG
HHHHHHCCCCEEECHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCC
TQRGAQKIAEEATRRGLEISVIGIPKTIDNDISYIQATFGFETAVAEARRATYAAHTEAD
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEEHHHCHHHHHHHHHHHHHHHHCHHH
AARNSISLVKLMGRDSGFIAAYSVLVNSHVNFCLVPEARFTLDGLLRALKERLERRGHAV
HHHCHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEE
VVVAEGAGQNLMEATAERDASGNIKYGDIGIFLRDAIRDYFRKASLEINLKYIDPSYIIR
EEEECCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHH
SMPANPHDAAFCLLLGQSAVHAGMSGRTNMVVSFWNREFTHVPITLAVSQRKKIDTSSML
CCCCCCCHHEEEEEECCHHHHCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHH
WNSVLAVTGQPDMM
HHHHEEECCCCCCC
>Mature Secondary Structure
MRQEGDMGNTELDFTIERLGECHIPSPVKGIRFVDDDGRVLYHSHLQEMKPWLDEGIHPP
CCCCCCCCCCCCEEEHHHCCCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCC
AMEAAGPREKVFFDPSRIACGIVTCGGICPGLNDVIRSIVLSLCHHYCVQKIYGFRFGYE
CCCCCCCCCCEEECCCHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLVQRYGHKPLELTADSVAQIHEIGGTILGSSRGPQEPSEMVKTLEDLNVGILFTIGGDG
HHHHHHCCCCEEECHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCC
TQRGAQKIAEEATRRGLEISVIGIPKTIDNDISYIQATFGFETAVAEARRATYAAHTEAD
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEEHHHCHHHHHHHHHHHHHHHHCHHH
AARNSISLVKLMGRDSGFIAAYSVLVNSHVNFCLVPEARFTLDGLLRALKERLERRGHAV
HHHCHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEE
VVVAEGAGQNLMEATAERDASGNIKYGDIGIFLRDAIRDYFRKASLEINLKYIDPSYIIR
EEEECCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHH
SMPANPHDAAFCLLLGQSAVHAGMSGRTNMVVSFWNREFTHVPITLAVSQRKKIDTSSML
CCCCCCCHHEEEEEECCHHHHCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHH
WNSVLAVTGQPDMM
HHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]