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The map label for this gene is ruvB

Identifier: 85858587

GI number: 85858587

Start: 748581

End: 749603

Strand: Direct

Name: ruvB

Synonym: SYN_02971

Alternate gene names: 85858587

Gene position: 748581-749603 (Clockwise)

Preceding gene: 85858586

Following gene: 85858588

Centisome position: 23.55

GC content: 43.79

Gene sequence:

>1023_bases
ATGAAGAGAGATGATCTGGTGAGTCCTGAATGCATGGATGGAGATAATGTTTATGAAACGACATTACGTCCCCATAGCAT
CAAGGAATATGTTGGTCAGAAAAAAATAAAGAAAACTCTGCCCATTTTTGTTGAGGCGGCCCAAAAAAGGAAAGAGGCCC
TCGATCATGTCCTGCTTTATGGACCTCCCGGTTTGGGTAAAACAACGCTGGCGCTTATTATTGCCAGAGAAATGGGATTC
AATATCAAGGTAACTTCGGGACCTGTTATTGAACGTCCGGGAGATCTGGCGGCAATTCTGACAAATCTGAAAGATTATGA
CATCCTCTTCATTGATGAAATTCATCGACTTCCTCATTCCGTCGAAGAAATCCTGTACCCGGCGATGGAAGACTTTTATA
TCGACATTGTGATTGGGCAAGGGCCTTCCGCACGAAGTATGAAATTGGACATCCCGAAGTTTACATTGGTGGGTGCGACA
ACAAGAGCCGGGTTACTTACTTCGCCACTGAGAGACCGGTTTGGGATAAGTTTCAGACTTGATTATTATGCTGTTGAAGA
ATTGACGAAGATCATAAACCGTTCTGCAAGCATTATGTCCATTGAAATCGAGCATTCAGGCGCTCTCGAAATAGCAAAAC
GTTCAAGAGGTACGCCGAGAATTGCCAATCGTCTCCTTAAAAGGGTCAGAGACTATGCCCAGGTAAAGGGGGAGGGGATA
ATAAAAAGGGCTGTCGCTGTTCACGCGCTGGAAATGCTGGAAATTGATGACAGAGGATTTGATCAAATGGATCGGAGTAT
TCTTTTGTCCATAATCGAGAATTACGGTGGCGGACCAGTAGGCATTGACACATTATGTGCTACAGTCGGGGAAGAAAAAA
CGACGATTGAAGACGTTTATGAGCCTTATCTGATAAAGGAAGGCTATCTACAAAAGACGGCACGCGGCAGGATAGCCACA
AAAAAAGCGTACGAACATTTCGGGAAAAGAAAATTCGAAGTTGGACAAAAGGAATTATTTTAA

Upstream 100 bases:

>100_bases
CTGGGGTAATTGGATATTAGGTGTCGCAGAGCGTTTGTTTGAATGGACTCTAATGTTTGTATTTTAAAAAATAAATAAAG
TATCCATTTTATGGAAGGCT

Downstream 100 bases:

>100_bases
TTTCAGTAATCTCTGTTTATGAAAATATTAATGCATGTCTGCTGTGCGCCCTGCACGATTTATCCTTTAAAGTTTCTGAG
GGACTGCAACCATGATGTTC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKRDDLVSPECMDGDNVYETTLRPHSIKEYVGQKKIKKTLPIFVEAAQKRKEALDHVLLYGPPGLGKTTLALIIAREMGF
NIKVTSGPVIERPGDLAAILTNLKDYDILFIDEIHRLPHSVEEILYPAMEDFYIDIVIGQGPSARSMKLDIPKFTLVGAT
TRAGLLTSPLRDRFGISFRLDYYAVEELTKIINRSASIMSIEIEHSGALEIAKRSRGTPRIANRLLKRVRDYAQVKGEGI
IKRAVAVHALEMLEIDDRGFDQMDRSILLSIIENYGGGPVGIDTLCATVGEEKTTIEDVYEPYLIKEGYLQKTARGRIAT
KKAYEHFGKRKFEVGQKELF

Sequences:

>Translated_340_residues
MKRDDLVSPECMDGDNVYETTLRPHSIKEYVGQKKIKKTLPIFVEAAQKRKEALDHVLLYGPPGLGKTTLALIIAREMGF
NIKVTSGPVIERPGDLAAILTNLKDYDILFIDEIHRLPHSVEEILYPAMEDFYIDIVIGQGPSARSMKLDIPKFTLVGAT
TRAGLLTSPLRDRFGISFRLDYYAVEELTKIINRSASIMSIEIEHSGALEIAKRSRGTPRIANRLLKRVRDYAQVKGEGI
IKRAVAVHALEMLEIDDRGFDQMDRSILLSIIENYGGGPVGIDTLCATVGEEKTTIEDVYEPYLIKEGYLQKTARGRIAT
KKAYEHFGKRKFEVGQKELF
>Mature_340_residues
MKRDDLVSPECMDGDNVYETTLRPHSIKEYVGQKKIKKTLPIFVEAAQKRKEALDHVLLYGPPGLGKTTLALIIAREMGF
NIKVTSGPVIERPGDLAAILTNLKDYDILFIDEIHRLPHSVEEILYPAMEDFYIDIVIGQGPSARSMKLDIPKFTLVGAT
TRAGLLTSPLRDRFGISFRLDYYAVEELTKIINRSASIMSIEIEHSGALEIAKRSRGTPRIANRLLKRVRDYAQVKGEGI
IKRAVAVHALEMLEIDDRGFDQMDRSILLSIIENYGGGPVGIDTLCATVGEEKTTIEDVYEPYLIKEGYLQKTARGRIAT
KKAYEHFGKRKFEVGQKELF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=328, Percent_Identity=58.5365853658537, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1787119, Length=138, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_SYNAS (Q2LRA8)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460789.1
- ProteinModelPortal:   Q2LRA8
- SMR:   Q2LRA8
- STRING:   Q2LRA8
- GeneID:   3885055
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02971
- NMPDR:   fig|56780.10.peg.730
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q2LRA8
- ProtClustDB:   PRK00080
- BioCyc:   SACI56780:SYN_02971-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38228; Mature: 38228

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDDLVSPECMDGDNVYETTLRPHSIKEYVGQKKIKKTLPIFVEAAQKRKEALDHVLLY
CCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
GPPGLGKTTLALIIAREMGFNIKVTSGPVIERPGDLAAILTNLKDYDILFIDEIHRLPHS
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHCHHH
VEEILYPAMEDFYIDIVIGQGPSARSMKLDIPKFTLVGATTRAGLLTSPLRDRFGISFRL
HHHHHHHHHHCEEEEEEECCCCCCCCEEECCCCEEEEECCHHCCHHHHHHHHHCCCEEEE
DYYAVEELTKIINRSASIMSIEIEHSGALEIAKRSRGTPRIANRLLKRVRDYAQVKGEGI
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
IKRAVAVHALEMLEIDDRGFDQMDRSILLSIIENYGGGPVGIDTLCATVGEEKTTIEDVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHH
EPYLIKEGYLQKTARGRIATKKAYEHFGKRKFEVGQKELF
CCHHHCCHHHHHHHCCCHHHHHHHHHHCCHHHHCCHHHCC
>Mature Secondary Structure
MKRDDLVSPECMDGDNVYETTLRPHSIKEYVGQKKIKKTLPIFVEAAQKRKEALDHVLLY
CCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
GPPGLGKTTLALIIAREMGFNIKVTSGPVIERPGDLAAILTNLKDYDILFIDEIHRLPHS
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHCHHH
VEEILYPAMEDFYIDIVIGQGPSARSMKLDIPKFTLVGATTRAGLLTSPLRDRFGISFRL
HHHHHHHHHHCEEEEEEECCCCCCCCEEECCCCEEEEECCHHCCHHHHHHHHHCCCEEEE
DYYAVEELTKIINRSASIMSIEIEHSGALEIAKRSRGTPRIANRLLKRVRDYAQVKGEGI
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
IKRAVAVHALEMLEIDDRGFDQMDRSILLSIIENYGGGPVGIDTLCATVGEEKTTIEDVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHH
EPYLIKEGYLQKTARGRIATKKAYEHFGKRKFEVGQKELF
CCHHHCCHHHHHHHCCCHHHHHHHHHHCCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA