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The map label for this gene is ruvC [H]

Identifier: 85858585

GI number: 85858585

Start: 747141

End: 747686

Strand: Direct

Name: ruvC [H]

Synonym: SYN_02973

Alternate gene names: 85858585

Gene position: 747141-747686 (Clockwise)

Preceding gene: 85858584

Following gene: 85858586

Centisome position: 23.5

GC content: 39.56

Gene sequence:

>546_bases
TTGTCTGCATTCGGGTTATGCGTAAAGGTTAACTTTAAAATACCACATGAGTCATGGATTATGAGATTGAAAGTGTTGGG
AATTGATCCGGGAAGTGTTGTTACGGGTTTTGGAATCGTGGAGCAAATTCAGGAGCGGTATATTGATATTGTCCATGGAG
AAATCAGGTTAAAAAGAGGAACATCATTCACTTCATCATTGGAGAAAATCTATGATGAATTGTTTTCTCTTGTAGAACGT
GAACAACCTGATGCGGTCGCGATTGAGGATATATTTTACGGTAAAAATATCAAGAGTTTAATCAAGCAGGGGCACGTAAG
GGGGGTGGCTATTTTGGCAGGTTCAAAACATCATTTGCCTGTGTTTGAATACACGCCGCTTGAAATAAAAAAAGCAGTGG
TCGGCTATGGTCATGCTGAGAAAATTCAAGTACAGAACATGATAAAAGTCATTATGAAATTGTCAGAGCTTCCTCCCGCA
GATGCTTCAGACGCAATCGCAGTAGCCATATGTCATCTCAACCATCAGAAGAAAAATCACATATAA

Upstream 100 bases:

>100_bases
TTTATTATCAATAAAGAGCAATAAAAATCTTTAAAGATAATGCAAAAGCATCATAATTGTCAAAGAAATGTGACTGATGA
CTCTCTGAGCACTTGGTTGA

Downstream 100 bases:

>100_bases
TCCATGATTGCGCAGATTCGAGGAAAACTGATACTTAAAACCGTATCGATGGTTATCATTGATAATCACGGGATCGGCTA
TGAGGTAATGATTCCGCTAT

Product: crossover junction endodeoxyribonuclease

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSAFGLCVKVNFKIPHESWIMRLKVLGIDPGSVVTGFGIVEQIQERYIDIVHGEIRLKRGTSFTSSLEKIYDELFSLVER
EQPDAVAIEDIFYGKNIKSLIKQGHVRGVAILAGSKHHLPVFEYTPLEIKKAVVGYGHAEKIQVQNMIKVIMKLSELPPA
DASDAIAVAICHLNHQKKNHI

Sequences:

>Translated_181_residues
MSAFGLCVKVNFKIPHESWIMRLKVLGIDPGSVVTGFGIVEQIQERYIDIVHGEIRLKRGTSFTSSLEKIYDELFSLVER
EQPDAVAIEDIFYGKNIKSLIKQGHVRGVAILAGSKHHLPVFEYTPLEIKKAVVGYGHAEKIQVQNMIKVIMKLSELPPA
DASDAIAVAICHLNHQKKNHI
>Mature_180_residues
SAFGLCVKVNFKIPHESWIMRLKVLGIDPGSVVTGFGIVEQIQERYIDIVHGEIRLKRGTSFTSSLEKIYDELFSLVERE
QPDAVAIEDIFYGKNIKSLIKQGHVRGVAILAGSKHHLPVFEYTPLEIKKAVVGYGHAEKIQVQNMIKVIMKLSELPPAD
ASDAIAVAICHLNHQKKNHI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=134, Percent_Identity=44.0298507462687, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 20238; Mature: 20107

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFGLCVKVNFKIPHESWIMRLKVLGIDPGSVVTGFGIVEQIQERYIDIVHGEIRLKRG
CCCCEEEEEEEEECCHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECC
TSFTSSLEKIYDELFSLVEREQPDAVAIEDIFYGKNIKSLIKQGHVRGVAILAGSKHHLP
CCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCC
VFEYTPLEIKKAVVGYGHAEKIQVQNMIKVIMKLSELPPADASDAIAVAICHLNHQKKNH
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
I
C
>Mature Secondary Structure 
SAFGLCVKVNFKIPHESWIMRLKVLGIDPGSVVTGFGIVEQIQERYIDIVHGEIRLKRG
CCCEEEEEEEEECCHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECC
TSFTSSLEKIYDELFSLVEREQPDAVAIEDIFYGKNIKSLIKQGHVRGVAILAGSKHHLP
CCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCC
VFEYTPLEIKKAVVGYGHAEKIQVQNMIKVIMKLSELPPADASDAIAVAICHLNHQKKNH
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA