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The map label for this gene is gyrB [H]

Identifier: 85857848

GI number: 85857848

Start: 2927

End: 5329

Strand: Direct

Name: gyrB [H]

Synonym: SYN_02049

Alternate gene names: 85857848

Gene position: 2927-5329 (Clockwise)

Preceding gene: 85857847

Following gene: 85857849

Centisome position: 0.09

GC content: 52.39

Gene sequence:

>2403_bases
ATGACAGACAATTATTCCGCTGACAGCATTAAAATACTGGAAGGTCTGGAAGCCGTAAGGGTCAGACCGGCCATGTATAT
CGGCAGTATCGGCAAGGAAGGATTGCACCACCTGGTGTATGAAGTGGTGGATAACAGTATCGATGAAGCTTCGGCAGGAT
ACTGTGATAAAATTACAGTAAAAATAAGAGTCGATAACAGCATCATGGTGGAAGACAACGGCCGGGGCATTCCCGTTGAT
ATGCATGAAACGGGAGTTTCAGCCGCCGAGGTCGTGATGACCAAACTGCATGCCGGGGGAAAATTTTCCAATGATTCCTA
CAAGATTTCCGGTGGGCTTCACGGTGTCGGCGTTTCCGTCGTCAATGCCCTTTCCAGCTCCATGGAACTCAATGTCCGTC
GAGACGGAAAGGTCTATTCCCAGTCTTATGTCCGCGGCGTTCCAACAGCTCCTCTGGCCGTCACCGGTCAGACCTCGGGA
AGAGGTACCAAGATTTACTTCAAACCCGACGATACGATCTTTGAGGAACTGGAATTCAATTTCGACATTCTGGCCAACCG
ATTGCGGGAACTGGCGTTTCTGAACTCCGGTGTCCGTATCGCGCTGATCGATGACCGCAGCGACCGGAAGAGTGAATTCT
TTTATGAAGGAGGTATTATCTCCTTTGTCGAGTATATCAATCGCAACAAAAAGGTTCTGCACAAGGATCCCATTTACGTC
AGCGGAGAGAGGGAAGACTCCACGGTGGAAGTGGCGCTGCAGTACAACGATACCTATACGGAAAATATCTTCTCATTCGC
CAACAGCATCAACACCACCGAAGGAGGAACGCATCTGAGCGGATTCCGGTCGGCCCTGACCCGGGTGTTCAATAACTACG
CGGTCAACAACGGCCTTTTGAAGAATGGAAAGGAATCGCTGCGGGGGGAAGACCTGCGGGAAGGACTTGCCTGTGTGATC
AGCGTCAAGGTCCAGAATCCTCAGTTCGAAGGTCAGACCAAGACCAAACTGGGCAACAGCGAAGTGCGCGGCCTGGTCGA
GGGCATCGTCTATGAAAAGATCGGCAGCTACCTTGAGGAAAATCCCTCTGTTGCCAAACAACTGCTGAACAAATGTCTCG
AGGCCTCACGAGCCCGGGAAGCAGCCCGGCGGGCAAGGGAACTGACACGCCGGAAAAGCGCCCTGGAGGTGGGGGCTCTG
CCCGGCAAACTGGCGGACTGTCAGGAGCGGGATCCGGCAAGAAGTGAGCTTTACCTGGTGGAGGGGGATTCTGCAGGCGG
CTCCGCCAAGCAGGGTCGCGATCGACGGAATCAGGCCATTCTGCCCCTGCGGGGCAAAGTGTTGAATGTGGAAAAGGCCC
GCTTCGACAAGATGCTTCAGAACGAGGAAATCAAGACCATGATAACCGCCCTGGGAACGGGAATCGGCCAGGAGGATTAT
GATGTCAGTCGAATCCGCTACCACAAGGTCATTATTATGACCGATGCCGATGTGGACGGTTCTCATATCCGCACCCTTCT
GCTGACTTTTTTCTTTCGCCAGATGCGGGAGCTCGTGGAAAAGGGCTATCTCTATATCGCTCAGCCGCCGCTTTTCAAGA
TCACGGAAAAGAAAAACGAAGTTTACATTCGCAACGAGGAAGAAATGAACAATTTCATCCTCCAGAACGGTGTCAAACGG
ATCAAGCTGTTTAATGGGCGCGAAGAGGCGGTCACCGGCAACCAGCTGCTCAATCTGGTCAAGAAGGTGATGCGGGTCGA
GACCATTCTTGACAAGTTCGAAAAAGAGGATTGCGACCGTAAGATTATACTTGCTCTCGCGGAGAGACCCTCTTTCACCA
TTCAGGACTTCGATGATGAAGCGCTTCTGAAGGAAGTGGCCCGCCAGACCGCGCAAGCTCTCGGAGAAGAGGTCAGGGAA
GCCGGGGTGGAAGCGGATGAGGAACACGGCGGATACAAACTGTTTTTCTTCCTGCAGAATAATGGGAAGGTGCAGCGGAT
TTCCTTTGGAAAATCCATTTACCGGGCGCCGAAATTCTCGGAAGTCAAAGCGATTCTGGAACAGATTTCCTCTGTCGGAA
AACCGCCCTATTCGGTCATGGCCATTGACGGGGATGGAACGGACCGTCAGGAAATTCCGGATATGTCATCCCTGGTGCAG
TTTGTCAATTCCGTGGGAAAAAAAGGGCTCTCGGTCCAGCGCTACAAGGGTCTGGGCGAAATGAATCCAGAACAGCTATG
GGAAACCACGATGAACCCGGAGAAGAGAACCCTCCTGCAGGTTCGTGTGGATGATGCCGTGCAGGCGGATGAAATTTTCA
CCACATTGATGGGGGATCAGGTTGAACCCCGTAGAGAATTCATTTACCAGAACGCTCTTTACGTGTCCAACCTGGATGTC
TAG

Upstream 100 bases:

>100_bases
AATCAGGGAACCGGCCACTAATGAAAAATCCCTGAAAAATCAAAAAAATAAATTCTGTATCGATGAAATAAAAAGCAGTC
GGGAAGAGAGAGTAGATAAA

Downstream 100 bases:

>100_bases
AAGGAAGGAATAAAAGAATGGATGCATTGTCAATTCCAAGAAACATTCTTGTAAATATAGAAGATGAAATGAAAAAGTCC
TACATGGACTATGCTATGAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MTDNYSADSIKILEGLEAVRVRPAMYIGSIGKEGLHHLVYEVVDNSIDEASAGYCDKITVKIRVDNSIMVEDNGRGIPVD
MHETGVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSVVNALSSSMELNVRRDGKVYSQSYVRGVPTAPLAVTGQTSG
RGTKIYFKPDDTIFEELEFNFDILANRLRELAFLNSGVRIALIDDRSDRKSEFFYEGGIISFVEYINRNKKVLHKDPIYV
SGEREDSTVEVALQYNDTYTENIFSFANSINTTEGGTHLSGFRSALTRVFNNYAVNNGLLKNGKESLRGEDLREGLACVI
SVKVQNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEENPSVAKQLLNKCLEASRAREAARRARELTRRKSALEVGAL
PGKLADCQERDPARSELYLVEGDSAGGSAKQGRDRRNQAILPLRGKVLNVEKARFDKMLQNEEIKTMITALGTGIGQEDY
DVSRIRYHKVIIMTDADVDGSHIRTLLLTFFFRQMRELVEKGYLYIAQPPLFKITEKKNEVYIRNEEEMNNFILQNGVKR
IKLFNGREEAVTGNQLLNLVKKVMRVETILDKFEKEDCDRKIILALAERPSFTIQDFDDEALLKEVARQTAQALGEEVRE
AGVEADEEHGGYKLFFFLQNNGKVQRISFGKSIYRAPKFSEVKAILEQISSVGKPPYSVMAIDGDGTDRQEIPDMSSLVQ
FVNSVGKKGLSVQRYKGLGEMNPEQLWETTMNPEKRTLLQVRVDDAVQADEIFTTLMGDQVEPRREFIYQNALYVSNLDV

Sequences:

>Translated_800_residues
MTDNYSADSIKILEGLEAVRVRPAMYIGSIGKEGLHHLVYEVVDNSIDEASAGYCDKITVKIRVDNSIMVEDNGRGIPVD
MHETGVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSVVNALSSSMELNVRRDGKVYSQSYVRGVPTAPLAVTGQTSG
RGTKIYFKPDDTIFEELEFNFDILANRLRELAFLNSGVRIALIDDRSDRKSEFFYEGGIISFVEYINRNKKVLHKDPIYV
SGEREDSTVEVALQYNDTYTENIFSFANSINTTEGGTHLSGFRSALTRVFNNYAVNNGLLKNGKESLRGEDLREGLACVI
SVKVQNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEENPSVAKQLLNKCLEASRAREAARRARELTRRKSALEVGAL
PGKLADCQERDPARSELYLVEGDSAGGSAKQGRDRRNQAILPLRGKVLNVEKARFDKMLQNEEIKTMITALGTGIGQEDY
DVSRIRYHKVIIMTDADVDGSHIRTLLLTFFFRQMRELVEKGYLYIAQPPLFKITEKKNEVYIRNEEEMNNFILQNGVKR
IKLFNGREEAVTGNQLLNLVKKVMRVETILDKFEKEDCDRKIILALAERPSFTIQDFDDEALLKEVARQTAQALGEEVRE
AGVEADEEHGGYKLFFFLQNNGKVQRISFGKSIYRAPKFSEVKAILEQISSVGKPPYSVMAIDGDGTDRQEIPDMSSLVQ
FVNSVGKKGLSVQRYKGLGEMNPEQLWETTMNPEKRTLLQVRVDDAVQADEIFTTLMGDQVEPRREFIYQNALYVSNLDV
>Mature_799_residues
TDNYSADSIKILEGLEAVRVRPAMYIGSIGKEGLHHLVYEVVDNSIDEASAGYCDKITVKIRVDNSIMVEDNGRGIPVDM
HETGVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSVVNALSSSMELNVRRDGKVYSQSYVRGVPTAPLAVTGQTSGR
GTKIYFKPDDTIFEELEFNFDILANRLRELAFLNSGVRIALIDDRSDRKSEFFYEGGIISFVEYINRNKKVLHKDPIYVS
GEREDSTVEVALQYNDTYTENIFSFANSINTTEGGTHLSGFRSALTRVFNNYAVNNGLLKNGKESLRGEDLREGLACVIS
VKVQNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEENPSVAKQLLNKCLEASRAREAARRARELTRRKSALEVGALP
GKLADCQERDPARSELYLVEGDSAGGSAKQGRDRRNQAILPLRGKVLNVEKARFDKMLQNEEIKTMITALGTGIGQEDYD
VSRIRYHKVIIMTDADVDGSHIRTLLLTFFFRQMRELVEKGYLYIAQPPLFKITEKKNEVYIRNEEEMNNFILQNGVKRI
KLFNGREEAVTGNQLLNLVKKVMRVETILDKFEKEDCDRKIILALAERPSFTIQDFDDEALLKEVARQTAQALGEEVREA
GVEADEEHGGYKLFFFLQNNGKVQRISFGKSIYRAPKFSEVKAILEQISSVGKPPYSVMAIDGDGTDRQEIPDMSSLVQF
VNSVGKKGLSVQRYKGLGEMNPEQLWETTMNPEKRTLLQVRVDDAVQADEIFTTLMGDQVEPRREFIYQNALYVSNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=577, Percent_Identity=25.1299826689775, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI19913408, Length=618, Percent_Identity=25.5663430420712, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI48994957, Length=830, Percent_Identity=51.0843373493976, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=578, Percent_Identity=40.3114186851211, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=674, Percent_Identity=23.8872403560831, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=534, Percent_Identity=24.1573033707865, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=172, Percent_Identity=30.2325581395349, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=588, Percent_Identity=26.530612244898, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17136538, Length=611, Percent_Identity=27.4959083469722, Blast_Score=150, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89702; Mature: 89571

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNYSADSIKILEGLEAVRVRPAMYIGSIGKEGLHHLVYEVVDNSIDEASAGYCDKITV
CCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
KIRVDNSIMVEDNGRGIPVDMHETGVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSV
EEEECCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
VNALSSSMELNVRRDGKVYSQSYVRGVPTAPLAVTGQTSGRGTKIYFKPDDTIFEELEFN
HHHHHHCCEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCHHHHHHCCC
FDILANRLRELAFLNSGVRIALIDDRSDRKSEFFYEGGIISFVEYINRNKKVLHKDPIYV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCCHHHHHHHHCCCCCEEECCCEEE
SGEREDSTVEVALQYNDTYTENIFSFANSINTTEGGTHLSGFRSALTRVFNNYAVNNGLL
ECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
KNGKESLRGEDLREGLACVISVKVQNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEE
HCCHHHCCCHHHHCCEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NPSVAKQLLNKCLEASRAREAARRARELTRRKSALEVGALPGKLADCQERDPARSELYLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
EGDSAGGSAKQGRDRRNQAILPLRGKVLNVEKARFDKMLQNEEIKTMITALGTGIGQEDY
ECCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
DVSRIRYHKVIIMTDADVDGSHIRTLLLTFFFRQMRELVEKGYLYIAQPPLFKITEKKNE
CHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCE
VYIRNEEEMNNFILQNGVKRIKLFNGREEAVTGNQLLNLVKKVMRVETILDKFEKEDCDR
EEEECHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KIILALAERPSFTIQDFDDEALLKEVARQTAQALGEEVREAGVEADEEHGGYKLFFFLQN
EEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEC
NGKVQRISFGKSIYRAPKFSEVKAILEQISSVGKPPYSVMAIDGDGTDRQEIPDMSSLVQ
CCCEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCHHHHHH
FVNSVGKKGLSVQRYKGLGEMNPEQLWETTMNPEKRTLLQVRVDDAVQADEIFTTLMGDQ
HHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHCCC
VEPRREFIYQNALYVSNLDV
CCHHHHHHHHCCEEEECCCC
>Mature Secondary Structure 
TDNYSADSIKILEGLEAVRVRPAMYIGSIGKEGLHHLVYEVVDNSIDEASAGYCDKITV
CCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
KIRVDNSIMVEDNGRGIPVDMHETGVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSV
EEEECCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
VNALSSSMELNVRRDGKVYSQSYVRGVPTAPLAVTGQTSGRGTKIYFKPDDTIFEELEFN
HHHHHHCCEEEEECCCCHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCHHHHHHCCC
FDILANRLRELAFLNSGVRIALIDDRSDRKSEFFYEGGIISFVEYINRNKKVLHKDPIYV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCCHHHHHHHHCCCCCEEECCCEEE
SGEREDSTVEVALQYNDTYTENIFSFANSINTTEGGTHLSGFRSALTRVFNNYAVNNGLL
ECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
KNGKESLRGEDLREGLACVISVKVQNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEE
HCCHHHCCCHHHHCCEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NPSVAKQLLNKCLEASRAREAARRARELTRRKSALEVGALPGKLADCQERDPARSELYLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
EGDSAGGSAKQGRDRRNQAILPLRGKVLNVEKARFDKMLQNEEIKTMITALGTGIGQEDY
ECCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
DVSRIRYHKVIIMTDADVDGSHIRTLLLTFFFRQMRELVEKGYLYIAQPPLFKITEKKNE
CHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCE
VYIRNEEEMNNFILQNGVKRIKLFNGREEAVTGNQLLNLVKKVMRVETILDKFEKEDCDR
EEEECHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KIILALAERPSFTIQDFDDEALLKEVARQTAQALGEEVREAGVEADEEHGGYKLFFFLQN
EEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEC
NGKVQRISFGKSIYRAPKFSEVKAILEQISSVGKPPYSVMAIDGDGTDRQEIPDMSSLVQ
CCCEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCHHHHHH
FVNSVGKKGLSVQRYKGLGEMNPEQLWETTMNPEKRTLLQVRVDDAVQADEIFTTLMGDQ
HHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHCCC
VEPRREFIYQNALYVSNLDV
CCHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]