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The map label for this gene is dnaN [H]

Identifier: 85857847

GI number: 85857847

Start: 1699

End: 2817

Strand: Direct

Name: dnaN [H]

Synonym: SYN_02050

Alternate gene names: 85857847

Gene position: 1699-2817 (Clockwise)

Preceding gene: 85857846

Following gene: 85857848

Centisome position: 0.05

GC content: 49.96

Gene sequence:

>1119_bases
ATGGAATTTGATATTCAGCGACAGGTTTTTCTGGATGGCGTTCAAAAAACCCTCGGAATTGTGGAAAGAAAAAGTACCAT
GCCCATTCTCGGAAATCTGCTGCTCAAAACGGAAGACAACCAGATCAAGATTGTCGCCACGGATCGGGAACTGGGTCTGG
TTGCCGATTACGAGGCGCAGATCATCAGCAGCGGGGCAATCACCCTTTCCGCGAAAAAGCTGTTTGAAATGATCCGGGAA
ATCCAGGGGGACAGCATTCATTTCTCCTGCGATGATCATGATCAGATTACCCTGAGCAGTCAGAAAGCGGTCTACCGGAT
ACCCGGCATTTCCGCTGATGAGTTTCCCGCTGTGGCATATGATGAAAACTTGCCGCTGTATCCGGTTCCGGCATCCCTTT
TAAAGGAATTGATTTCCAAAATCTCCTTTGCCATTTCGAATGATGAGACGAGAAAAAATCTGACCGGCGCCCTGATGGAG
GCAGAGCCGGGGGAACAGGGGACCATTCTCCGAATGGTCGCGACGGATGGACATCGCCTGGCCAGGAGTTACGAAGTTAT
AGAAGGTAATTCTCCCTTCACTCTGGAAAAAGGGATTATTCTTCCCCGAAAGGGAATTTCCGAGATCCGCAAGCTGATCG
ATGCCGAGCAGGGGGAAATCCTGATCGGGTCGGAACGCGGGATGTTTGTTCTGAAAACAGCCAATACCCTGTTGAAGGTG
AGTCTCATTGATGAAACCTACCCGGATTACACGAGGGTCATTCCCAGCCAGCAGGGAGTCCGGGTGATTTTCAATCACGA
TCTTCTTTTGCATGGCCTCCGGCGGATGAAGGTCATCTCGACTGAACAGTACAGCGGCGTCGTGCTCACCTTGAATAATA
ACAGAATGATTATGAATTCGACGAACCCTGATGTGGGAGAAGCCAACGACGAACTGGAGGTTGCCTACCAGGGAGAGGAG
GTCAAGGTCGGCTTCAACGTGGATTATCTGATTGACGCCATAGAGGTCATCCGGGAAGAAGAAGTGCTGTTTGATATCGG
ATCGTTCATGAAACCCAGCATGATCATGCCTGTTCACAACGACCGCTTTCTGTGCGTGGTCATGCCTTTAAAGCTTTAA

Upstream 100 bases:

>100_bases
TATAAAGACTTATCCACAGGTCTTATTACTAAAACTAATTTAAATAAATAAACAAAGAATAAAAAATAAAATATAAGATC
GAAAGGAATGGGTCAGGATC

Downstream 100 bases:

>100_bases
AACCGGTTGAATCAGGGAACCGGCCACTAATGAAAAATCCCTGAAAAATCAAAAAAATAAATTCTGTATCGATGAAATAA
AAAGCAGTCGGGAAGAGAGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEFDIQRQVFLDGVQKTLGIVERKSTMPILGNLLLKTEDNQIKIVATDRELGLVADYEAQIISSGAITLSAKKLFEMIRE
IQGDSIHFSCDDHDQITLSSQKAVYRIPGISADEFPAVAYDENLPLYPVPASLLKELISKISFAISNDETRKNLTGALME
AEPGEQGTILRMVATDGHRLARSYEVIEGNSPFTLEKGIILPRKGISEIRKLIDAEQGEILIGSERGMFVLKTANTLLKV
SLIDETYPDYTRVIPSQQGVRVIFNHDLLLHGLRRMKVISTEQYSGVVLTLNNNRMIMNSTNPDVGEANDELEVAYQGEE
VKVGFNVDYLIDAIEVIREEEVLFDIGSFMKPSMIMPVHNDRFLCVVMPLKL

Sequences:

>Translated_372_residues
MEFDIQRQVFLDGVQKTLGIVERKSTMPILGNLLLKTEDNQIKIVATDRELGLVADYEAQIISSGAITLSAKKLFEMIRE
IQGDSIHFSCDDHDQITLSSQKAVYRIPGISADEFPAVAYDENLPLYPVPASLLKELISKISFAISNDETRKNLTGALME
AEPGEQGTILRMVATDGHRLARSYEVIEGNSPFTLEKGIILPRKGISEIRKLIDAEQGEILIGSERGMFVLKTANTLLKV
SLIDETYPDYTRVIPSQQGVRVIFNHDLLLHGLRRMKVISTEQYSGVVLTLNNNRMIMNSTNPDVGEANDELEVAYQGEE
VKVGFNVDYLIDAIEVIREEEVLFDIGSFMKPSMIMPVHNDRFLCVVMPLKL
>Mature_372_residues
MEFDIQRQVFLDGVQKTLGIVERKSTMPILGNLLLKTEDNQIKIVATDRELGLVADYEAQIISSGAITLSAKKLFEMIRE
IQGDSIHFSCDDHDQITLSSQKAVYRIPGISADEFPAVAYDENLPLYPVPASLLKELISKISFAISNDETRKNLTGALME
AEPGEQGTILRMVATDGHRLARSYEVIEGNSPFTLEKGIILPRKGISEIRKLIDAEQGEILIGSERGMFVLKTANTLLKV
SLIDETYPDYTRVIPSQQGVRVIFNHDLLLHGLRRMKVISTEQYSGVVLTLNNNRMIMNSTNPDVGEANDELEVAYQGEE
VKVGFNVDYLIDAIEVIREEEVLFDIGSFMKPSMIMPVHNDRFLCVVMPLKL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=374, Percent_Identity=29.144385026738, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41633; Mature: 41633

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDIQRQVFLDGVQKTLGIVERKSTMPILGNLLLKTEDNQIKIVATDRELGLVADYEAQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEECCCCE
IISSGAITLSAKKLFEMIREIQGDSIHFSCDDHDQITLSSQKAVYRIPGISADEFPAVAY
EEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCEEEECCCCCCCCCCCEEE
DENLPLYPVPASLLKELISKISFAISNDETRKNLTGALMEAEPGEQGTILRMVATDGHRL
CCCCCCEECCHHHHHHHHHHHEEEECCCCHHHHHHEEEEECCCCCCCCEEEEEECCCHHH
ARSYEVIEGNSPFTLEKGIILPRKGISEIRKLIDAEQGEILIGSERGMFVLKTANTLLKV
HHEEEEEECCCCEEEECCEECCCCCHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEEEE
SLIDETYPDYTRVIPSQQGVRVIFNHDLLLHGLRRMKVISTEQYSGVVLTLNNNRMIMNS
EEECCCCCCHHHCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCEEEEECCCEEEEEC
TNPDVGEANDELEVAYQGEEVKVGFNVDYLIDAIEVIREEEVLFDIGSFMKPSMIMPVHN
CCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DRFLCVVMPLKL
CCEEEEEEEECC
>Mature Secondary Structure
MEFDIQRQVFLDGVQKTLGIVERKSTMPILGNLLLKTEDNQIKIVATDRELGLVADYEAQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCEEECCCCE
IISSGAITLSAKKLFEMIREIQGDSIHFSCDDHDQITLSSQKAVYRIPGISADEFPAVAY
EEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCEEEECCCCCCCCCCCEEE
DENLPLYPVPASLLKELISKISFAISNDETRKNLTGALMEAEPGEQGTILRMVATDGHRL
CCCCCCEECCHHHHHHHHHHHEEEECCCCHHHHHHEEEEECCCCCCCCEEEEEECCCHHH
ARSYEVIEGNSPFTLEKGIILPRKGISEIRKLIDAEQGEILIGSERGMFVLKTANTLLKV
HHEEEEEECCCCEEEECCEECCCCCHHHHHHHHCCCCCCEEEECCCCEEEEEECCEEEEE
SLIDETYPDYTRVIPSQQGVRVIFNHDLLLHGLRRMKVISTEQYSGVVLTLNNNRMIMNS
EEECCCCCCHHHCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCEEEEECCCEEEEEC
TNPDVGEANDELEVAYQGEEVKVGFNVDYLIDAIEVIREEEVLFDIGSFMKPSMIMPVHN
CCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DRFLCVVMPLKL
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]