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The map label for this gene is ntrC [H]

Identifier: 85374082

GI number: 85374082

Start: 1305411

End: 1306823

Strand: Reverse

Name: ntrC [H]

Synonym: ELI_06275

Alternate gene names: 85374082

Gene position: 1306823-1305411 (Counterclockwise)

Preceding gene: 85374083

Following gene: 85374081

Centisome position: 42.81

GC content: 64.61

Gene sequence:

>1413_bases
ATGAGCGATCCCATTCTCGTGGTCGAAGACGACGAAAGCATCGCCACCGTCATCGTCAGTGCGCTCGAGGGCGAGGGCTA
TGCCGTCGACCAGTGCGCCTCGATCGCCCGGCGCGACGACTTGCTCGCCGCGCGAAACTACGCGGCGATGCTCACCGACG
TGATCCTGGAGGATGGCGACGGCATTGCTTCGCTCGACCGGGTGCGGGCGGCGCATTCCGCCATGCCGATCATCATCCTG
TCGGCGCAGAACACGCTCGATACGGCTGTGCGGGCGAGCGAAAACGATGCCTTTGAATATTTTCCCAAGCCTTTCGACCT
CGAAGAACTGATCCGCGCGGTCCGCCAGGCGGCCGGGGCGGGCAAGCAGGAACGACCGCTCGAAATCGACGATGCCAGCG
CGCTGCCGCTGGTCGGTCGGAGCAAGGCGATGCAGGGCGTTTTCCGGATGATAACCCGCGTCCTGCGCAACGATCTCACA
GTCTTGGTGCTGGGGGAATCCGGGACCGGCAAGGAATTGGTGGCCGAGGCTATCCACCAGCTTGGCGCACGCAAGACCGG
ACCGTTCGTGGCTGTCAACGCCGCCGCGATCCCTCGCGAACTGATCGAGAGCGAATTGTTCGGGCACGAGAAGGGCGCAT
TCACCGGCGCGGTCAATCAGGCCATCGGCAAGTTCGAACAGGCCAACGGCGGGACGCTGTTTCTCGACGAAATCGGCGAC
ATGCCCATCGAAGCGCAGACGCGGTTGCTGCGTGCGCTCCAATCGGGCCGCATCCGCCGAGTTGGCGGGCGGCAGGATGT
TGCGGTCAATGTGCGCATCGTGGCTGCAACCAATCGCGACCTCGCACCCATGATTGCCAGCGGGCAATTCCGCGAGGATC
TGTACTACCGTCTCAACGTAGTGCCGATCGAACTGCCGCCGCTGCGCGCGCGGCGTGACGATATCGGGGCTCTGACGCGG
CATTTCCTCGCGCTTGCAGCCGACGAAGGCCTTCCGCTACGCACGATCGACGAAGCTGCCATTGCAGTGCTGGAGCGTCA
GCCCTGGCGGGGCAATGTCAGGGAATTGCGCAACCTCGTCTATCGGCTTGCCCTGCTGGCCCGCAACGACGTGATCGATG
CGGAACTGGCGGAAGAGGTGCTCGATCACGAAGGCGGGCCCTCGACCATGGAGGGGCCTTCGTCGGTCGATCGCGCCATG
CTCGACTGGATCGAAGAAAACCGGCCCGCGCCCGGGGCACTCTATCGCGCGGCGTCGGCGGCGTTCGAGAAACCGCTGTT
CGAGCATGCGCTGCGCGCCACCGCCGGAAACCAGCTGCGGGCAGCGGAATTGCTCGGGATCAACCGCAACACGCTGCGCA
AGAGACTGGGCGAACTCGGTATCGTCGCCGAGACGTTCTCTCCACGGGGCTAG

Upstream 100 bases:

>100_bases
TGGTGGCCGACATGGATGGTCGTATCGCTTACGAGCGCGACCGCGTCGCCGAACTGACCAATTTCCGCATCAACCTCGCC
ATGGCACCGAAGGAGCCGAC

Downstream 100 bases:

>100_bases
TTGCAGAAAAGCAACAAATTTCGCTTGCATATGGGCAACACCGTCGTTGTAACTGCGCAACGATGCAGGACGGGGACCAG
ACAGTTCAGCGCAGCCCGCG

Product: NtrC, nitrogen regulation protein NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDPILVVEDDESIATVIVSALEGEGYAVDQCASIARRDDLLAARNYAAMLTDVILEDGDGIASLDRVRAAHSAMPIIIL
SAQNTLDTAVRASENDAFEYFPKPFDLEELIRAVRQAAGAGKQERPLEIDDASALPLVGRSKAMQGVFRMITRVLRNDLT
VLVLGESGTGKELVAEAIHQLGARKTGPFVAVNAAAIPRELIESELFGHEKGAFTGAVNQAIGKFEQANGGTLFLDEIGD
MPIEAQTRLLRALQSGRIRRVGGRQDVAVNVRIVAATNRDLAPMIASGQFREDLYYRLNVVPIELPPLRARRDDIGALTR
HFLALAADEGLPLRTIDEAAIAVLERQPWRGNVRELRNLVYRLALLARNDVIDAELAEEVLDHEGGPSTMEGPSSVDRAM
LDWIEENRPAPGALYRAASAAFEKPLFEHALRATAGNQLRAAELLGINRNTLRKRLGELGIVAETFSPRG

Sequences:

>Translated_470_residues
MSDPILVVEDDESIATVIVSALEGEGYAVDQCASIARRDDLLAARNYAAMLTDVILEDGDGIASLDRVRAAHSAMPIIIL
SAQNTLDTAVRASENDAFEYFPKPFDLEELIRAVRQAAGAGKQERPLEIDDASALPLVGRSKAMQGVFRMITRVLRNDLT
VLVLGESGTGKELVAEAIHQLGARKTGPFVAVNAAAIPRELIESELFGHEKGAFTGAVNQAIGKFEQANGGTLFLDEIGD
MPIEAQTRLLRALQSGRIRRVGGRQDVAVNVRIVAATNRDLAPMIASGQFREDLYYRLNVVPIELPPLRARRDDIGALTR
HFLALAADEGLPLRTIDEAAIAVLERQPWRGNVRELRNLVYRLALLARNDVIDAELAEEVLDHEGGPSTMEGPSSVDRAM
LDWIEENRPAPGALYRAASAAFEKPLFEHALRATAGNQLRAAELLGINRNTLRKRLGELGIVAETFSPRG
>Mature_469_residues
SDPILVVEDDESIATVIVSALEGEGYAVDQCASIARRDDLLAARNYAAMLTDVILEDGDGIASLDRVRAAHSAMPIIILS
AQNTLDTAVRASENDAFEYFPKPFDLEELIRAVRQAAGAGKQERPLEIDDASALPLVGRSKAMQGVFRMITRVLRNDLTV
LVLGESGTGKELVAEAIHQLGARKTGPFVAVNAAAIPRELIESELFGHEKGAFTGAVNQAIGKFEQANGGTLFLDEIGDM
PIEAQTRLLRALQSGRIRRVGGRQDVAVNVRIVAATNRDLAPMIASGQFREDLYYRLNVVPIELPPLRARRDDIGALTRH
FLALAADEGLPLRTIDEAAIAVLERQPWRGNVRELRNLVYRLALLARNDVIDAELAEEVLDHEGGPSTMEGPSSVDRAML
DWIEENRPAPGALYRAASAAFEKPLFEHALRATAGNQLRAAELLGINRNTLRKRLGELGIVAETFSPRG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=463, Percent_Identity=44.060475161987, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=466, Percent_Identity=37.5536480686695, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=40.3508771929825, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=36.4224137931034, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=44.3768996960486, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=43.161094224924, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1789233, Length=250, Percent_Identity=48.4, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=40.3076923076923, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=40.1197604790419, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=39.1437308868502, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=251, Percent_Identity=42.6294820717131, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789828, Length=349, Percent_Identity=32.0916905444126, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=31.1111111111111, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1790552, Length=124, Percent_Identity=35.4838709677419, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=128, Percent_Identity=35.15625, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51112; Mature: 50981

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPILVVEDDESIATVIVSALEGEGYAVDQCASIARRDDLLAARNYAAMLTDVILEDGD
CCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIASLDRVRAAHSAMPIIILSAQNTLDTAVRASENDAFEYFPKPFDLEELIRAVRQAAGA
CHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
GKQERPLEIDDASALPLVGRSKAMQGVFRMITRVLRNDLTVLVLGESGTGKELVAEAIHQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
LGARKTGPFVAVNAAAIPRELIESELFGHEKGAFTGAVNQAIGKFEQANGGTLFLDEIGD
HCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
MPIEAQTRLLRALQSGRIRRVGGRQDVAVNVRIVAATNRDLAPMIASGQFREDLYYRLNV
CCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHCCEEEEEE
VPIELPPLRARRDDIGALTRHFLALAADEGLPLRTIDEAAIAVLERQPWRGNVRELRNLV
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
YRLALLARNDVIDAELAEEVLDHEGGPSTMEGPSSVDRAMLDWIEENRPAPGALYRAASA
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFEKPLFEHALRATAGNQLRAAELLGINRNTLRKRLGELGIVAETFSPRG
HHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCEEECCCCCCC
>Mature Secondary Structure 
SDPILVVEDDESIATVIVSALEGEGYAVDQCASIARRDDLLAARNYAAMLTDVILEDGD
CCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIASLDRVRAAHSAMPIIILSAQNTLDTAVRASENDAFEYFPKPFDLEELIRAVRQAAGA
CHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
GKQERPLEIDDASALPLVGRSKAMQGVFRMITRVLRNDLTVLVLGESGTGKELVAEAIHQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
LGARKTGPFVAVNAAAIPRELIESELFGHEKGAFTGAVNQAIGKFEQANGGTLFLDEIGD
HCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
MPIEAQTRLLRALQSGRIRRVGGRQDVAVNVRIVAATNRDLAPMIASGQFREDLYYRLNV
CCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCCCHHHHCCCHHHCCEEEEEE
VPIELPPLRARRDDIGALTRHFLALAADEGLPLRTIDEAAIAVLERQPWRGNVRELRNLV
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
YRLALLARNDVIDAELAEEVLDHEGGPSTMEGPSSVDRAMLDWIEENRPAPGALYRAASA
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFEKPLFEHALRATAGNQLRAAELLGINRNTLRKRLGELGIVAETFSPRG
HHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]