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The map label for this gene is ntrB [H]

Identifier: 85374083

GI number: 85374083

Start: 1306820

End: 1307893

Strand: Reverse

Name: ntrB [H]

Synonym: ELI_06280

Alternate gene names: 85374083

Gene position: 1307893-1306820 (Counterclockwise)

Preceding gene: 85374084

Following gene: 85374082

Centisome position: 42.85

GC content: 66.01

Gene sequence:

>1074_bases
GTGAGCGACGCGCCCGCGCGGCCCGATCCGCGCGCGCAAATCGCCGGGATGGTCTTTGCCGTGTTGCTGATCGACCCGGA
CGCCGTCATCGCCGAAGTGAACCCTGCCGCAGAGGACCTGCTCGGGCGCAGCGCGCGCAAGCTGCTCGGCGCAAACCTGT
TCGATGCCATCGAAATTACCGACGAGCGCGTTTCGGAGCGGATGGCGACGGGCGATTCCATGCTCACCGCGCGCGGCCTC
CCGATCGGTACGCCCGGCGGGGCGCGGCAGGTCAATCTCACCATGTCGCCGCTCTCGACCCATCCGGGCTGGAGGATCGT
CACGCTATCCGAGATCGGCCAGGCCGATATGGATCGCGGCTATGACGAGACCGCTTTCGCCGCCCCCTCGGTGCTGGCGC
ACGAGATCAAGAACCCGCTGTCCGCCATTCGCGGGGCCGGGCAGCTGCTTGTGCGCAAACTGCCGGAAAGCGAGCGGCCC
TTTGCGCTCCTGATCGCCAAGGAAGTCGATCGCATCGCGCGGCTCGTCGACCGGATGCAGGCGCTCGGCAGCCGCAGTTC
GGATCCGATGGAGGAAGTCAATCTGCACGAGATAGTCCGCGCGGCGCTCACAGTCGTCCGTTCTGCCGACGATCGCGGCG
TGCAGTTCGAGGAAGAGTTCGATCCCTCGCTGCCCAAGGTATGGGCCAGCCCGGATACGCTCCAGCAGGTACTGATCAAT
CTGCTCAGCAATGCCCGCGATGCCTGCGCAGACGGGGATGAAGGGGGGACGGTGACAGTCCGCACCCGGTTCGTCAGTGG
GCTGGTGATGAACATGCTGAGACCCGGACGCTCGGTGCGCTTGCCGATCGAGCTGACGGTGATCGACAACGGGCCGGGGA
TCGATCCGCACCTGGGCGATCGCGTGTTCGAACCCTTCGTGACCTCGAAGAAGGGCGGGCAGGGCCTGGGGCTTGCCCTC
GTCCGCAAGCTGGTGGCCGACATGGATGGTCGTATCGCTTACGAGCGCGACCGCGTCGCCGAACTGACCAATTTCCGCAT
CAACCTCGCCATGGCACCGAAGGAGCCGACATGA

Upstream 100 bases:

>100_bases
CGGCCGAATTCCGCAATTTCGTCAATTTCATCGACGATCCCGACCAGGTTCTGGGCGAGATCGAGCGGTTCTACACGCCC
TTCCTGCAGCGGCGGGCGGC

Downstream 100 bases:

>100_bases
GCGATCCCATTCTCGTGGTCGAAGACGACGAAAGCATCGCCACCGTCATCGTCAGTGCGCTCGAGGGCGAGGGCTATGCC
GTCGACCAGTGCGCCTCGAT

Product: nitrogen regulatory signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSDAPARPDPRAQIAGMVFAVLLIDPDAVIAEVNPAAEDLLGRSARKLLGANLFDAIEITDERVSERMATGDSMLTARGL
PIGTPGGARQVNLTMSPLSTHPGWRIVTLSEIGQADMDRGYDETAFAAPSVLAHEIKNPLSAIRGAGQLLVRKLPESERP
FALLIAKEVDRIARLVDRMQALGSRSSDPMEEVNLHEIVRAALTVVRSADDRGVQFEEEFDPSLPKVWASPDTLQQVLIN
LLSNARDACADGDEGGTVTVRTRFVSGLVMNMLRPGRSVRLPIELTVIDNGPGIDPHLGDRVFEPFVTSKKGGQGLGLAL
VRKLVADMDGRIAYERDRVAELTNFRINLAMAPKEPT

Sequences:

>Translated_357_residues
MSDAPARPDPRAQIAGMVFAVLLIDPDAVIAEVNPAAEDLLGRSARKLLGANLFDAIEITDERVSERMATGDSMLTARGL
PIGTPGGARQVNLTMSPLSTHPGWRIVTLSEIGQADMDRGYDETAFAAPSVLAHEIKNPLSAIRGAGQLLVRKLPESERP
FALLIAKEVDRIARLVDRMQALGSRSSDPMEEVNLHEIVRAALTVVRSADDRGVQFEEEFDPSLPKVWASPDTLQQVLIN
LLSNARDACADGDEGGTVTVRTRFVSGLVMNMLRPGRSVRLPIELTVIDNGPGIDPHLGDRVFEPFVTSKKGGQGLGLAL
VRKLVADMDGRIAYERDRVAELTNFRINLAMAPKEPT
>Mature_356_residues
SDAPARPDPRAQIAGMVFAVLLIDPDAVIAEVNPAAEDLLGRSARKLLGANLFDAIEITDERVSERMATGDSMLTARGLP
IGTPGGARQVNLTMSPLSTHPGWRIVTLSEIGQADMDRGYDETAFAAPSVLAHEIKNPLSAIRGAGQLLVRKLPESERPF
ALLIAKEVDRIARLVDRMQALGSRSSDPMEEVNLHEIVRAALTVVRSADDRGVQFEEEFDPSLPKVWASPDTLQQVLINL
LSNARDACADGDEGGTVTVRTRFVSGLVMNMLRPGRSVRLPIELTVIDNGPGIDPHLGDRVFEPFVTSKKGGQGLGLALV
RKLVADMDGRIAYERDRVAELTNFRINLAMAPKEPT

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=356, Percent_Identity=31.7415730337079, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1788549, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790436, Length=204, Percent_Identity=32.3529411764706, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=32.2429906542056, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38651; Mature: 38520

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPARPDPRAQIAGMVFAVLLIDPDAVIAEVNPAAEDLLGRSARKLLGANLFDAIEIT
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
DERVSERMATGDSMLTARGLPIGTPGGARQVNLTMSPLSTHPGWRIVTLSEIGQADMDRG
HHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCHHHHCC
YDETAFAAPSVLAHEIKNPLSAIRGAGQLLVRKLPESERPFALLIAKEVDRIARLVDRMQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
ALGSRSSDPMEEVNLHEIVRAALTVVRSADDRGVQFEEEFDPSLPKVWASPDTLQQVLIN
HHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHH
LLSNARDACADGDEGGTVTVRTRFVSGLVMNMLRPGRSVRLPIELTVIDNGPGIDPHLGD
HHHCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCH
RVFEPFVTSKKGGQGLGLALVRKLVADMDGRIAYERDRVAELTNFRINLAMAPKEPT
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHHCEEEEEEECCCCCC
>Mature Secondary Structure 
SDAPARPDPRAQIAGMVFAVLLIDPDAVIAEVNPAAEDLLGRSARKLLGANLFDAIEIT
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
DERVSERMATGDSMLTARGLPIGTPGGARQVNLTMSPLSTHPGWRIVTLSEIGQADMDRG
HHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCHHHHCC
YDETAFAAPSVLAHEIKNPLSAIRGAGQLLVRKLPESERPFALLIAKEVDRIARLVDRMQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
ALGSRSSDPMEEVNLHEIVRAALTVVRSADDRGVQFEEEFDPSLPKVWASPDTLQQVLIN
HHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHH
LLSNARDACADGDEGGTVTVRTRFVSGLVMNMLRPGRSVRLPIELTVIDNGPGIDPHLGD
HHHCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCH
RVFEPFVTSKKGGQGLGLALVRKLVADMDGRIAYERDRVAELTNFRINLAMAPKEPT
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHHCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]