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The map label for this gene is ribB [H]

Identifier: 85373980

GI number: 85373980

Start: 1189426

End: 1190673

Strand: Reverse

Name: ribB [H]

Synonym: ELI_05765

Alternate gene names: 85373980

Gene position: 1190673-1189426 (Counterclockwise)

Preceding gene: 85373981

Following gene: 85373979

Centisome position: 39.01

GC content: 61.54

Gene sequence:

>1248_bases
ATGAGTTCATCGATTATCGAACGTGTGCGCGACCTTGTTTCGTCCGGCACTATGACCAAGGCCGGTCTGGCACGCGCAGC
GGGCCTCCACGCCAACACCCTGCGCGATGCCAGCGAGCCGGATTGGAACCCCACGACCGAGACGCTTGGCAAGCTCGAGA
ACTTCCTTATCCAGAACGACGACCGCCCCGTCCTTGTCGGAATCGAGGAGATCATCGACGAAGCGCGCAACGGGCGGATG
TATATCCTGGTCGATGACGAGGATCGCGAGAACGAAGGCGATCTCATCATTCCCGGCCAGATGGCGACGCCCATGGCGAT
CAATTTCATGGCCACCCACGGGCGTGGCCTGATCTGTCTCGCGCTCGACAAAGCGCGGGTGAAGAAGCTCGGGCTAGAGC
CGATGAGCCGCAACAACCGCGAAAGCATGCAGACCGCCTTCACCACCTCTATCGAGGCCAAGGAAGGGGTCACCACCGGC
ATCTCCGCCGCCGATCGCGCGCGCACCGTCGCGGTGGCCATCGACAGCACCAAGGGCCCGGACGACATCGTCACCCCAGG
TCATGTATTCCCGCTGACTGCGCGCGATGGCGGTGTTCTCGTTCGTGCCGGCCATACCGAGGCGGCGGTCGATATCTCCC
GCCTCGCCGGTCTCAACCCCTCGGGCGTGATCTGCGAGATCCTGAACGAGGATGGCTCCATGGCCCGGCTCGACGATCTC
ATTATTTTCGCGCGCAAGCACGATTTGAAGATCGGCACCATCCGGGACCTGATCGAGTACCGCATGCGCCACGATCACCT
GGTCCAGCGCATCGAAGAACGCGACTTCGAAAGCGATTATGGCGGACAGTGGAAGATGGCGGTCTACCGCAACTCGATCG
ACGGGCGCGAGCATTATGTGCTGCAGAAAGGGCATGTCGTACCCGGCGAACCTACCCTCGCCCGCGTCCATCCCATTTCG
ATCTTCGACGATGTCCTCGGCCAGCCCGGCGAGCGCAAGCGCACGCTCCAGCGCTCGATGCAGGCGATCGGAGAACATGG
CTCGGGCGTCATTGTGATCATTACCGGGCGTCAGGCTACCGGCAGCTACAGCGACGATGACGAAGTCCGCAATGTCGGCA
TCGGCTCTCAGATCCTCGCCGATCTCGACGTCCACGACATGATCCTGCTCAGCAATTCACAGCCCAATGTGGTGGCGATC
GAAGGCTACGGCCTCAATATCGTCGATCACAAGTCCATCCCGGAGTAA

Upstream 100 bases:

>100_bases
CTGCGCTGCGTGGACCGTGTCGACCTAGCGCGCAGCTTTTCACACGCAGGATCACATCATGCGGGCTTGTAGTCACATTC
AAATGTGATACGGTCTGCGC

Downstream 100 bases:

>100_bases
TTCATGGCCCGTTTTCTGATCGTCGAAGCGCGTTTCTACGACCATCTCAACGATATGCTTGTGGCCGGTGCCCGCGCCGC
GCTCGAGGCGGAGGGGCATG

Product: GTP cyclohydrolase II

Products: NA

Alternate protein names: DHBP synthase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSSSIIERVRDLVSSGTMTKAGLARAAGLHANTLRDASEPDWNPTTETLGKLENFLIQNDDRPVLVGIEEIIDEARNGRM
YILVDDEDRENEGDLIIPGQMATPMAINFMATHGRGLICLALDKARVKKLGLEPMSRNNRESMQTAFTTSIEAKEGVTTG
ISAADRARTVAVAIDSTKGPDDIVTPGHVFPLTARDGGVLVRAGHTEAAVDISRLAGLNPSGVICEILNEDGSMARLDDL
IIFARKHDLKIGTIRDLIEYRMRHDHLVQRIEERDFESDYGGQWKMAVYRNSIDGREHYVLQKGHVVPGEPTLARVHPIS
IFDDVLGQPGERKRTLQRSMQAIGEHGSGVIVIITGRQATGSYSDDDEVRNVGIGSQILADLDVHDMILLSNSQPNVVAI
EGYGLNIVDHKSIPE

Sequences:

>Translated_415_residues
MSSSIIERVRDLVSSGTMTKAGLARAAGLHANTLRDASEPDWNPTTETLGKLENFLIQNDDRPVLVGIEEIIDEARNGRM
YILVDDEDRENEGDLIIPGQMATPMAINFMATHGRGLICLALDKARVKKLGLEPMSRNNRESMQTAFTTSIEAKEGVTTG
ISAADRARTVAVAIDSTKGPDDIVTPGHVFPLTARDGGVLVRAGHTEAAVDISRLAGLNPSGVICEILNEDGSMARLDDL
IIFARKHDLKIGTIRDLIEYRMRHDHLVQRIEERDFESDYGGQWKMAVYRNSIDGREHYVLQKGHVVPGEPTLARVHPIS
IFDDVLGQPGERKRTLQRSMQAIGEHGSGVIVIITGRQATGSYSDDDEVRNVGIGSQILADLDVHDMILLSNSQPNVVAI
EGYGLNIVDHKSIPE
>Mature_414_residues
SSSIIERVRDLVSSGTMTKAGLARAAGLHANTLRDASEPDWNPTTETLGKLENFLIQNDDRPVLVGIEEIIDEARNGRMY
ILVDDEDRENEGDLIIPGQMATPMAINFMATHGRGLICLALDKARVKKLGLEPMSRNNRESMQTAFTTSIEAKEGVTTGI
SAADRARTVAVAIDSTKGPDDIVTPGHVFPLTARDGGVLVRAGHTEAAVDISRLAGLNPSGVICEILNEDGSMARLDDLI
IFARKHDLKIGTIRDLIEYRMRHDHLVQRIEERDFESDYGGQWKMAVYRNSIDGREHYVLQKGHVVPGEPTLARVHPISI
FDDVLGQPGERKRTLQRSMQAIGEHGSGVIVIITGRQATGSYSDDDEVRNVGIGSQILADLDVHDMILLSNSQPNVVAIE
GYGLNIVDHKSIPE

Specific function: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=198, Percent_Identity=48.989898989899, Blast_Score=199, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=38.3495145631068, Blast_Score=149, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12 [H]

Molecular weight: Translated: 45436; Mature: 45305

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSIIERVRDLVSSGTMTKAGLARAAGLHANTLRDASEPDWNPTTETLGKLENFLIQND
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DRPVLVGIEEIIDEARNGRMYILVDDEDRENEGDLIIPGQMATPMAINFMATHGRGLICL
CCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEEEECCCEEEEE
ALDKARVKKLGLEPMSRNNRESMQTAFTTSIEAKEGVTTGISAADRARTVAVAIDSTKGP
EECHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
DDIVTPGHVFPLTARDGGVLVRAGHTEAAVDISRLAGLNPSGVICEILNEDGSMARLDDL
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHE
IIFARKHDLKIGTIRDLIEYRMRHDHLVQRIEERDFESDYGGQWKMAVYRNSIDGREHYV
EEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEE
LQKGHVVPGEPTLARVHPISIFDDVLGQPGERKRTLQRSMQAIGEHGSGVIVIITGRQAT
EECCEECCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GSYSDDDEVRNVGIGSQILADLDVHDMILLSNSQPNVVAIEGYGLNIVDHKSIPE
CCCCCCHHHHHCCCCHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEECCCCCCC
>Mature Secondary Structure 
SSSIIERVRDLVSSGTMTKAGLARAAGLHANTLRDASEPDWNPTTETLGKLENFLIQND
CHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
DRPVLVGIEEIIDEARNGRMYILVDDEDRENEGDLIIPGQMATPMAINFMATHGRGLICL
CCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEEEECCCEEEEE
ALDKARVKKLGLEPMSRNNRESMQTAFTTSIEAKEGVTTGISAADRARTVAVAIDSTKGP
EECHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
DDIVTPGHVFPLTARDGGVLVRAGHTEAAVDISRLAGLNPSGVICEILNEDGSMARLDDL
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHE
IIFARKHDLKIGTIRDLIEYRMRHDHLVQRIEERDFESDYGGQWKMAVYRNSIDGREHYV
EEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEE
LQKGHVVPGEPTLARVHPISIFDDVLGQPGERKRTLQRSMQAIGEHGSGVIVIITGRQAT
EECCEECCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GSYSDDDEVRNVGIGSQILADLDVHDMILLSNSQPNVVAIEGYGLNIVDHKSIPE
CCCCCCHHHHHCCCCHHHHHCCCCEEEEEEECCCCCEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339274 [H]