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The map label for this gene is ribH

Identifier: 85373979

GI number: 85373979

Start: 1189000

End: 1189422

Strand: Reverse

Name: ribH

Synonym: ELI_05760

Alternate gene names: 85373979

Gene position: 1189422-1189000 (Counterclockwise)

Preceding gene: 85373980

Following gene: 85373976

Centisome position: 38.97

GC content: 65.48

Gene sequence:

>423_bases
ATGGCCCGTTTTCTGATCGTCGAAGCGCGTTTCTACGACCATCTCAACGATATGCTTGTGGCCGGTGCCCGCGCCGCGCT
CGAGGCGGAGGGGCATGAGGCTGAGGTGCTGACCGTCCCCGGCGCGCTCGAAGTGCCGGGCGCGATCGCCATGGCGGCGG
AAAACGGCGAGTTCGACGGCTTCGTCGCCATCGGTGTCGTGATCCGCGGCGAGACCTATCACTTCGAGATCGTCGCCGGC
GAAAGTGCCCGCGGCATCCTCGCGCTGACGATGGATGGCATTGCTATCGGCAATGGTATCCTGACGACCGAGAATGAAGA
GCAGGCTCTGGAGCGGGCCGACCCCGCGCGGAAGGACAAGGGCGGCGAAGCGGCCAAGGCCGCCATCGCCTTGCTCAAGT
TGCAGGACCGCTTCGGAGCCTGA

Upstream 100 bases:

>100_bases
CCACGACATGATCCTGCTCAGCAATTCACAGCCCAATGTGGTGGCGATCGAAGGCTACGGCCTCAATATCGTCGATCACA
AGTCCATCCCGGAGTAATTC

Downstream 100 bases:

>100_bases
CGCGAGCTACTCGGCCCCGTCGAGAAACTCCTTCAAGCCCAGCGGCGCGAACGGCCCGATGATCAGCTGTTCGAAAATGC
CGATGCCTTCGCGGCCATCG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MARFLIVEARFYDHLNDMLVAGARAALEAEGHEAEVLTVPGALEVPGAIAMAAENGEFDGFVAIGVVIRGETYHFEIVAG
ESARGILALTMDGIAIGNGILTTENEEQALERADPARKDKGGEAAKAAIALLKLQDRFGA

Sequences:

>Translated_140_residues
MARFLIVEARFYDHLNDMLVAGARAALEAEGHEAEVLTVPGALEVPGAIAMAAENGEFDGFVAIGVVIRGETYHFEIVAG
ESARGILALTMDGIAIGNGILTTENEEQALERADPARKDKGGEAAKAAIALLKLQDRFGA
>Mature_139_residues
ARFLIVEARFYDHLNDMLVAGARAALEAEGHEAEVLTVPGALEVPGAIAMAAENGEFDGFVAIGVVIRGETYHFEIVAGE
SARGILALTMDGIAIGNGILTTENEEQALERADPARKDKGGEAAKAAIALLKLQDRFGA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=138, Percent_Identity=42.0289855072464, Blast_Score=92, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_ERYLH (Q2NAP7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458041.1
- STRING:   Q2NAP7
- GeneID:   3870839
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05760
- NMPDR:   fig|314225.3.peg.1912
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   PRK00061
- BioCyc:   ELIT314225:ELI_05760-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 14721; Mature: 14590

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFLIVEARFYDHLNDMLVAGARAALEAEGHEAEVLTVPGALEVPGAIAMAAENGEFDG
CCEEEEEEHHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCC
FVAIGVVIRGETYHFEIVAGESARGILALTMDGIAIGNGILTTENEEQALERADPARKDK
EEEEEEEEECCEEEEEEEECCCCCEEEEEEECCEEECCCEEECCCHHHHHHHCCCCCCCC
GGEAAKAAIALLKLQDRFGA
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARFLIVEARFYDHLNDMLVAGARAALEAEGHEAEVLTVPGALEVPGAIAMAAENGEFDG
CEEEEEEHHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCC
FVAIGVVIRGETYHFEIVAGESARGILALTMDGIAIGNGILTTENEEQALERADPARKDK
EEEEEEEEECCEEEEEEEECCCCCEEEEEEECCEEECCCEEECCCHHHHHHHCCCCCCCC
GGEAAKAAIALLKLQDRFGA
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA