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The map label for this gene is ilvB [H]

Identifier: 85373930

GI number: 85373930

Start: 1145227

End: 1146984

Strand: Reverse

Name: ilvB [H]

Synonym: ELI_05515

Alternate gene names: 85373930

Gene position: 1146984-1145227 (Counterclockwise)

Preceding gene: 85373931

Following gene: 85373929

Centisome position: 37.58

GC content: 63.2

Gene sequence:

>1758_bases
ATGACCACCCCCTCCCCCGAAAGCAGTGGCGCGGCGATCCTCGTCGACTGCCTCGTGCGCGAGGGCGTGGAATTCGTCTT
CGGCTATCCCGGCGGTGCCGTGCTCCCGATCTACGACGAATTGTTCGGGGACGAGCGCGTCCGCCATATCCTCGTCCGGC
ACGAAGCGGGCGCGGCGCACGCGGCGGAAGGCTATGCCCGCAGCACCGGGAAGCCCGGCGTCGTCCTCGTCACCAGCGGC
CCGGGCGCGACCAATGCGGTCACCGGTATCGCGGACGCTTATATGGATTCGATCCCGCTGGTGGTCATCACGGGACAGGT
CCCCACTCCGCTGATCGGGACCGACGCGTTCCAGGAGGCAGACACGGTCGGCATCACGCGGCACTGCACCAAGCACAATT
ATCTGGTGAAGGACCCGGACCAGCTCGGACCTGTTCTGCGCGAAGCCTTCCGTATCGCGACGACGGGGCGGCCCGGCCCG
GTTCTGGTCGACATCCCCAAGGACGTTCAGATCGCCATGGCGGATGGCGACAGTGCAGAAAGGGCACCGCTCGCCTCGTC
GCGCTACCAGCCACGCACGGTTGCTGCTCCTGACGAGATCCTCGAAGCGGTCGAGATGCTCGCAACGGCGGAGCGCCCTG
TTTTCTACACCGGCGGCGGCGTCATCAATTCAGGGCCGAAGGCGAGCGAAGCGCTGCGCCAATTGCAATCGCTCACCAAT
GCACCTTTGACTTCGACCCTGATGGGTCTCGGCGCCTTTCCCGCCGACCATCCCGACTGGCTCGGTATGCTGGGTATGCA
CGGCACGTTCGAGGCCAACATGGCGATGAACCGCGCCGACCTGATCGTGGCTGTCGGTGCGCGGTTCGACGATCGTGTGA
CCGGCAGGCTCGATGCTTTCGCGCCCCAGGCCAAGAAGATTCACATCGATATCGACCGCGCCAACATTAACAAGGTGGTG
CCCGTCGACCTCGGCATCGTCGGCGATTGCGGGACTGTGCTGGAACAGATGATTGCCGCCTGGGGCGACCGCAAAACGCA
GGACCTGGGCGAATGGAAGGCCCGCATCGCCGGGTGGCGCGCGCGTGAATGCCTCGCCTATCCGGAGCGCGATGACGAGA
TCATGCCGCAAAAAGCGGTCGAGCGGCTGTTCGAACTGACGAAGGATCGCGACCCGATCATCACCACCGAAGTCGGCCAG
CACCAGATGTGGGCGGCGCAATACTTCCGTTTCTTTGGGCCGAACAAATGGCTGACAAGCGGTGGCCTCGGCACGATGGG
CTATGGCCTGCCCGCGGCGATCGGCGCACAGCTGGGCAATCCGGACAGCCTCGTGATCGATATTGCGGGCGAGGCGTCGA
TCCAGATGAACATTCAGGAGCTCGGCACCGCCAGCCAGTATCGCCTGCCGGTCAAGATCTTCATTCTCAACAATGAGTAT
ATGGGCATGGTCCGCCAGTGGCAGGAACTGACCTACGAAAGCCGCTATTCGAATTCCTACTCCGACAGCCTGCCCGATTT
CGTGAAGCTGGCCGAAAGCTATGGCTGGACCGGCATTCGCATCGAGGACAAAGCTGGTCTGGATGCCGGCATCCAGGCCA
TGCTCGATGCCGATGGGCCGGTGATCGTCGATTGCCTGGTCTCGAAGGAAGCCAATTGCTTCCCGATGATCCCGAGCGGG
GCTGCGCATACCGACATGCTGCTTTATGGCGACCAGGTCGAAGGCACGATGGACGACGAAGCCAAGGCTTTGGTGTAA

Upstream 100 bases:

>100_bases
GAGACTCGTAACAGGGCCTTGGACAAACCCCTTGGGCCCGACGTTCTCATTCGAACGCCGGGCCCTGCTTTTAGATACGA
CGCTCTGACGGAGACAGACA

Downstream 100 bases:

>100_bases
AGGGCAGCCACCATGAAGATCACAGCGAAAGAGGCCGAGCGCCATGTGCTCGCCGTCACCGTCGATAATGAGCCGGGTAT
CCTCGCCAAAATTACCGGGC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTTPSPESSGAAILVDCLVREGVEFVFGYPGGAVLPIYDELFGDERVRHILVRHEAGAAHAAEGYARSTGKPGVVLVTSG
PGATNAVTGIADAYMDSIPLVVITGQVPTPLIGTDAFQEADTVGITRHCTKHNYLVKDPDQLGPVLREAFRIATTGRPGP
VLVDIPKDVQIAMADGDSAERAPLASSRYQPRTVAAPDEILEAVEMLATAERPVFYTGGGVINSGPKASEALRQLQSLTN
APLTSTLMGLGAFPADHPDWLGMLGMHGTFEANMAMNRADLIVAVGARFDDRVTGRLDAFAPQAKKIHIDIDRANINKVV
PVDLGIVGDCGTVLEQMIAAWGDRKTQDLGEWKARIAGWRARECLAYPERDDEIMPQKAVERLFELTKDRDPIITTEVGQ
HQMWAAQYFRFFGPNKWLTSGGLGTMGYGLPAAIGAQLGNPDSLVIDIAGEASIQMNIQELGTASQYRLPVKIFILNNEY
MGMVRQWQELTYESRYSNSYSDSLPDFVKLAESYGWTGIRIEDKAGLDAGIQAMLDADGPVIVDCLVSKEANCFPMIPSG
AAHTDMLLYGDQVEGTMDDEAKALV

Sequences:

>Translated_585_residues
MTTPSPESSGAAILVDCLVREGVEFVFGYPGGAVLPIYDELFGDERVRHILVRHEAGAAHAAEGYARSTGKPGVVLVTSG
PGATNAVTGIADAYMDSIPLVVITGQVPTPLIGTDAFQEADTVGITRHCTKHNYLVKDPDQLGPVLREAFRIATTGRPGP
VLVDIPKDVQIAMADGDSAERAPLASSRYQPRTVAAPDEILEAVEMLATAERPVFYTGGGVINSGPKASEALRQLQSLTN
APLTSTLMGLGAFPADHPDWLGMLGMHGTFEANMAMNRADLIVAVGARFDDRVTGRLDAFAPQAKKIHIDIDRANINKVV
PVDLGIVGDCGTVLEQMIAAWGDRKTQDLGEWKARIAGWRARECLAYPERDDEIMPQKAVERLFELTKDRDPIITTEVGQ
HQMWAAQYFRFFGPNKWLTSGGLGTMGYGLPAAIGAQLGNPDSLVIDIAGEASIQMNIQELGTASQYRLPVKIFILNNEY
MGMVRQWQELTYESRYSNSYSDSLPDFVKLAESYGWTGIRIEDKAGLDAGIQAMLDADGPVIVDCLVSKEANCFPMIPSG
AAHTDMLLYGDQVEGTMDDEAKALV
>Mature_584_residues
TTPSPESSGAAILVDCLVREGVEFVFGYPGGAVLPIYDELFGDERVRHILVRHEAGAAHAAEGYARSTGKPGVVLVTSGP
GATNAVTGIADAYMDSIPLVVITGQVPTPLIGTDAFQEADTVGITRHCTKHNYLVKDPDQLGPVLREAFRIATTGRPGPV
LVDIPKDVQIAMADGDSAERAPLASSRYQPRTVAAPDEILEAVEMLATAERPVFYTGGGVINSGPKASEALRQLQSLTNA
PLTSTLMGLGAFPADHPDWLGMLGMHGTFEANMAMNRADLIVAVGARFDDRVTGRLDAFAPQAKKIHIDIDRANINKVVP
VDLGIVGDCGTVLEQMIAAWGDRKTQDLGEWKARIAGWRARECLAYPERDDEIMPQKAVERLFELTKDRDPIITTEVGQH
QMWAAQYFRFFGPNKWLTSGGLGTMGYGLPAAIGAQLGNPDSLVIDIAGEASIQMNIQELGTASQYRLPVKIFILNNEYM
GMVRQWQELTYESRYSNSYSDSLPDFVKLAESYGWTGIRIEDKAGLDAGIQAMLDADGPVIVDCLVSKEANCFPMIPSGA
AHTDMLLYGDQVEGTMDDEAKALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=24.2957746478873, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=25.3448275862069, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=49.2146596858639, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=44.2680776014109, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=35.4203935599284, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=29.5289855072464, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788716, Length=482, Percent_Identity=26.1410788381743, Blast_Score=152, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=583, Percent_Identity=27.1012006861063, Blast_Score=155, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=583, Percent_Identity=27.1012006861063, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=599, Percent_Identity=25.542570951586, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=47.6109215017065, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6320816, Length=476, Percent_Identity=23.9495798319328, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=493, Percent_Identity=23.3265720081136, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=326, Percent_Identity=26.9938650306748, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922626, Length=578, Percent_Identity=25.7785467128028, Blast_Score=146, Evalue=5e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63141; Mature: 63010

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSPESSGAAILVDCLVREGVEFVFGYPGGAVLPIYDELFGDERVRHILVRHEAGAAH
CCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEHHHHHCCHHHHHHEEEECCCCCCH
AAEGYARSTGKPGVVLVTSGPGATNAVTGIADAYMDSIPLVVITGQVPTPLIGTDAFQEA
HHCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHC
DTVGITRHCTKHNYLVKDPDQLGPVLREAFRIATTGRPGPVLVDIPKDVQIAMADGDSAE
CCCCHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCC
RAPLASSRYQPRTVAAPDEILEAVEMLATAERPVFYTGGGVINSGPKASEALRQLQSLTN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHHHHHHHHHHC
APLTSTLMGLGAFPADHPDWLGMLGMHGTFEANMAMNRADLIVAVGARFDDRVTGRLDAF
CCHHHHHHHCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCHHHC
APQAKKIHIDIDRANINKVVPVDLGIVGDCGTVLEQMIAAWGDRKTQDLGEWKARIAGWR
CCCCEEEEEEEECCCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
ARECLAYPERDDEIMPQKAVERLFELTKDRDPIITTEVGQHQMWAAQYFRFFGPNKWLTS
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEC
GGLGTMGYGLPAAIGAQLGNPDSLVIDIAGEASIQMNIQELGTASQYRLPVKIFILNNEY
CCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHCCCCCEECEEEEEEECCHH
MGMVRQWQELTYESRYSNSYSDSLPDFVKLAESYGWTGIRIEDKAGLDAGIQAMLDADGP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCCC
VIVDCLVSKEANCFPMIPSGAAHTDMLLYGDQVEGTMDDEAKALV
EEEEEEECCCCCCEEECCCCCCCCEEEEECCCCCCCCCCCHHCCC
>Mature Secondary Structure 
TTPSPESSGAAILVDCLVREGVEFVFGYPGGAVLPIYDELFGDERVRHILVRHEAGAAH
CCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEEHHHHHCCHHHHHHEEEECCCCCCH
AAEGYARSTGKPGVVLVTSGPGATNAVTGIADAYMDSIPLVVITGQVPTPLIGTDAFQEA
HHCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHC
DTVGITRHCTKHNYLVKDPDQLGPVLREAFRIATTGRPGPVLVDIPKDVQIAMADGDSAE
CCCCHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCC
RAPLASSRYQPRTVAAPDEILEAVEMLATAERPVFYTGGGVINSGPKASEALRQLQSLTN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHHHHHHHHHHC
APLTSTLMGLGAFPADHPDWLGMLGMHGTFEANMAMNRADLIVAVGARFDDRVTGRLDAF
CCHHHHHHHCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCHHHC
APQAKKIHIDIDRANINKVVPVDLGIVGDCGTVLEQMIAAWGDRKTQDLGEWKARIAGWR
CCCCEEEEEEEECCCCCEEEEECCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
ARECLAYPERDDEIMPQKAVERLFELTKDRDPIITTEVGQHQMWAAQYFRFFGPNKWLTS
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEC
GGLGTMGYGLPAAIGAQLGNPDSLVIDIAGEASIQMNIQELGTASQYRLPVKIFILNNEY
CCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHCCCCCEECEEEEEEECCHH
MGMVRQWQELTYESRYSNSYSDSLPDFVKLAESYGWTGIRIEDKAGLDAGIQAMLDADGP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCCC
VIVDCLVSKEANCFPMIPSGAAHTDMLLYGDQVEGTMDDEAKALV
EEEEEEECCCCCCEEECCCCCCCCEEEEECCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]