Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 85373931

GI number: 85373931

Start: 1147103

End: 1148056

Strand: Reverse

Name: miaA

Synonym: ELI_05520

Alternate gene names: 85373931

Gene position: 1148056-1147103 (Counterclockwise)

Preceding gene: 85373934

Following gene: 85373930

Centisome position: 37.61

GC content: 63.42

Gene sequence:

>954_bases
ATGGGGCAAACTCCGGAAGATTGGGGCGAAAAACCGCCGGTGGCGCTCATTGCAGGGCCGACCGCCAGCGGCAAGAGCGA
TGTCGCGGTGCGGCTGGCGCTGGCGCTGAAAAAGCACGGGCGCGACGCTGTCGTGATCAACGCCGACAGCGCACAGGTCT
ATGCCGATCTGCGCGTCCTGAGCGCGCGGCCCAGCGAAGATGAAATGCGCGGTGTCCCGCACGAACTTTTCGGGGAATGG
GACGGAGCCGAAGCCTGCTCTGCGGCCGAATGGGCTTCGCGTGCGAAGGAAGAGATTTCCAAGGCGCACTTCACAGGTGC
AGTGCCGATCCTTGTTGGCGGAACCGGATTGTATTTGCGCACGCTGCTGGAAGGTATCGCCCCGATCCCGCCGATCGATG
CCGCCATTCGCGAGGCGATTCGAGCGTTGCCGCAGGATGAAGCGCGCGCGGCGCTCGAACGCGAGGACCCCGATGCCTCC
AAGCGGCTCGCCCCCGCCGATGCCGCACGGACGGCGCGCGCGCTCGAAGTCGTGCGATCCACCGGCCGGACGCTGAGTAA
CTGGCAGCAGCACAAGAAAGGGGGCATCGGCGATCGCGTCGCGCTCCACCCCCTGATCATGCTCCCGGATCGGAAGTGGC
TCTATGCGCGCTGCGACCGCCGATTCGAGCTTATGCTGGATCGTGGCGCTGCCAGCGAGGTTGAGACTCTGCTCGCACGC
GATCTCGATCCGTCCCTGCCAGTGATGCGCGCTATCGGCGTGCGCGAAATCGCGGCCTGGTTGAAAGGCGAGATTGACCG
GCCCCAAATGATCGCTTCTGGCCAGCAAGCGACGCGCAATTATGCCAAGCGGCAGTATACCTGGTTCCGGCATCAGCCAC
CGCCGGACTGGCCTCGCATCGAAACTTTCGATTTCAAGCTGGAGCGTGTTTTTGATGGATTATTTCAATATTAG

Upstream 100 bases:

>100_bases
CTCGATGCGCGACGACCAGCCCTTGTCATCCCACGCCGCGCGGAGGTTCTTGATCCGCGTTTCCAACCCGGCGGGGTCTG
CTATCAGGCGGACGATGAGC

Downstream 100 bases:

>100_bases
AGTTTGACAGGACATATTGAGACTCGTAACAGGGCCTTGGACAAACCCCTTGGGCCCGACGTTCTCATTCGAACGCCGGG
CCCTGCTTTTAGATACGACG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGQTPEDWGEKPPVALIAGPTASGKSDVAVRLALALKKHGRDAVVINADSAQVYADLRVLSARPSEDEMRGVPHELFGEW
DGAEACSAAEWASRAKEEISKAHFTGAVPILVGGTGLYLRTLLEGIAPIPPIDAAIREAIRALPQDEARAALEREDPDAS
KRLAPADAARTARALEVVRSTGRTLSNWQQHKKGGIGDRVALHPLIMLPDRKWLYARCDRRFELMLDRGAASEVETLLAR
DLDPSLPVMRAIGVREIAAWLKGEIDRPQMIASGQQATRNYAKRQYTWFRHQPPPDWPRIETFDFKLERVFDGLFQY

Sequences:

>Translated_317_residues
MGQTPEDWGEKPPVALIAGPTASGKSDVAVRLALALKKHGRDAVVINADSAQVYADLRVLSARPSEDEMRGVPHELFGEW
DGAEACSAAEWASRAKEEISKAHFTGAVPILVGGTGLYLRTLLEGIAPIPPIDAAIREAIRALPQDEARAALEREDPDAS
KRLAPADAARTARALEVVRSTGRTLSNWQQHKKGGIGDRVALHPLIMLPDRKWLYARCDRRFELMLDRGAASEVETLLAR
DLDPSLPVMRAIGVREIAAWLKGEIDRPQMIASGQQATRNYAKRQYTWFRHQPPPDWPRIETFDFKLERVFDGLFQY
>Mature_316_residues
GQTPEDWGEKPPVALIAGPTASGKSDVAVRLALALKKHGRDAVVINADSAQVYADLRVLSARPSEDEMRGVPHELFGEWD
GAEACSAAEWASRAKEEISKAHFTGAVPILVGGTGLYLRTLLEGIAPIPPIDAAIREAIRALPQDEARAALEREDPDASK
RLAPADAARTARALEVVRSTGRTLSNWQQHKKGGIGDRVALHPLIMLPDRKWLYARCDRRFELMLDRGAASEVETLLARD
LDPSLPVMRAIGVREIAAWLKGEIDRPQMIASGQQATRNYAKRQYTWFRHQPPPDWPRIETFDFKLERVFDGLFQY

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=335, Percent_Identity=25.6716417910448, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=36.4912280701754, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI25144712, Length=276, Percent_Identity=25.7246376811594, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=28.4280936454849, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24649830, Length=277, Percent_Identity=26.3537906137184, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ERYLH (Q2NAU5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457993.1
- STRING:   Q2NAU5
- GeneID:   3870501
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05520
- NMPDR:   fig|314225.3.peg.1960
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q2NAU5
- ProtClustDB:   PRK00091
- BioCyc:   ELIT314225:ELI_05520-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35155; Mature: 35024

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTPEDWGEKPPVALIAGPTASGKSDVAVRLALALKKHGRDAVVINADSAQVYADLRVL
CCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SARPSEDEMRGVPHELFGEWDGAEACSAAEWASRAKEEISKAHFTGAVPILVGGTGLYLR
CCCCCHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
TLLEGIAPIPPIDAAIREAIRALPQDEARAALEREDPDASKRLAPADAARTARALEVVRS
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
TGRTLSNWQQHKKGGIGDRVALHPLIMLPDRKWLYARCDRRFELMLDRGAASEVETLLAR
HCCHHHHHHHHHCCCCCCHHHHCCEEECCCCCHHHHHHCCHHHHHCCCCCHHHHHHHHHH
DLDPSLPVMRAIGVREIAAWLKGEIDRPQMIASGQQATRNYAKRQYTWFRHQPPPDWPRI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
ETFDFKLERVFDGLFQY
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQTPEDWGEKPPVALIAGPTASGKSDVAVRLALALKKHGRDAVVINADSAQVYADLRVL
CCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SARPSEDEMRGVPHELFGEWDGAEACSAAEWASRAKEEISKAHFTGAVPILVGGTGLYLR
CCCCCHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
TLLEGIAPIPPIDAAIREAIRALPQDEARAALEREDPDASKRLAPADAARTARALEVVRS
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
TGRTLSNWQQHKKGGIGDRVALHPLIMLPDRKWLYARCDRRFELMLDRGAASEVETLLAR
HCCHHHHHHHHHCCCCCCHHHHCCEEECCCCCHHHHHHCCHHHHHCCCCCHHHHHHHHHH
DLDPSLPVMRAIGVREIAAWLKGEIDRPQMIASGQQATRNYAKRQYTWFRHQPPPDWPRI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCC
ETFDFKLERVFDGLFQY
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA