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The map label for this gene is hipO [H]

Identifier: 85373884

GI number: 85373884

Start: 1092167

End: 1093495

Strand: Direct

Name: hipO [H]

Synonym: ELI_05285

Alternate gene names: 85373884

Gene position: 1092167-1093495 (Clockwise)

Preceding gene: 85373883

Following gene: 85373888

Centisome position: 35.78

GC content: 63.28

Gene sequence:

>1329_bases
ATGAACTGGGTTCTGGGTTTAGCTGCAGTTTCGGCTCTTGCGCTGGTCGCGAGTGCTGCCTCGGCGAATGAACTGCGCGA
AGATGTCATGGGCGATATGCCGGGCCTGGTGGAGCTTTATAAAGACCTGCACGCCAACCCCGAGCTTAGTTTCCAGGAGT
TCGAAACGGCCAAGAAACTCGCCGACAGGGCTCGCGCTCTCGGGTTCGAGGTCACCGAGGGGGTCGGGCAGACCGGCGTA
GTCGCGGTCATGCGCAACGGCGAAGGCCCCACGGTCATGCTGCGCGCCGACATGGACGGGCTACCGGTAGTCGAGCAGAC
CGGACTGCCGTATGCGTCAAAGCGGACTGCGGTGCCTGCCTCCGGGGTCGAAACCGGCGTAATGCACGCCTGCGGTCACG
ATACGCATATGGCGGCGTGGGTCGGTGCGGCGCAATTGCTGTCGGAGCGGAAGGCCGAATGGTCGGGCACCCTGGTGATG
ATCCTGCAACCGGCGGAGGAGATCGGGCTGGGCGCTCTGGCAATGCTCGAAGACGGGCTTTACGAGCGCTTTCCCAAGCC
GGACTACGTGCTGGGTTTCCACGACGCGGCGCAGTTTCCTGCGGGCATGATCGGCTATTCGCCCGGCTACGCGCTGGCCA
ACGTTGACAGCGTCGACATCACTGTCAAAGGCGTCGGCGGGCATGGCGCGTACCCGCATACGGCCAAGGATCCGATCGTC
CTTGCAAGTGCGATCGTAATGAAGCTGCAGACGCTGGTGAGCCGCGAAAGCTCGCCGCTCGATCCGGCAGTCGTGACTGT
CGGTAGCTTCCTAGCCGGAGCCAAGCACAACATCATCCCGGACGAGGCGAAGCTACAGCTTACCGTGCGCTCGTACAGCG
ATGAATCGCGCCAGCTGCTGCTTGATGGCATCAAGCGGATCGTGAAGGGTGAGGCCATGGCGGCGGGCATGCCGGACGAC
AGGATGCCCGAGGTCAACGTCATCCTTCCGTACACGCCTTCGACCTACAACACGCCCGAATTCACAGAGCAGGTCATGGC
GAAGCTAACAGACCGCTTCGGCGAAGGGCGCGTGATGCAGGTACCTTCGGTCATGGGCGGCGAGGACTTCGGCCAGTTCT
ACCGCGCCGACCGCGAGAACATCGAATCGCTGATCTTCTGGGTCGGCGGCGTCCCGCAGGACGAGTTCGACGCAGCGCAG
CGTGGTGAGACGAAGCTGCCGTCGCTGCACAGCCCGTTCTGGGCACCCGATGCGGAAAAGGTGATTGCGACGGCTACCGA
AGCCATGGTCGCGTCGACGCTCGACCTGATGAGCGCGTCGGGAGGTTAG

Upstream 100 bases:

>100_bases
CTTCCGGCGGTTCACTGGAGTGGCGCTGCCAACAAGACAGTTTTTGGTCGCCAGAACGTAGGTTCGTGCGGTCCATCGCA
TCGGGAATTGGGGAGTGGAT

Downstream 100 bases:

>100_bases
CCGGGGCTGCTAGAAGTCGTCGCCGGCATCGGACGCCAGATCGACCGCCGCGCTCGAACCGCCGGTTCTTGCACGCGATG
CTTGCGTCAGCCGCTCCATC

Product: amidohydrolase family protein

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNWVLGLAAVSALALVASAASANELREDVMGDMPGLVELYKDLHANPELSFQEFETAKKLADRARALGFEVTEGVGQTGV
VAVMRNGEGPTVMLRADMDGLPVVEQTGLPYASKRTAVPASGVETGVMHACGHDTHMAAWVGAAQLLSERKAEWSGTLVM
ILQPAEEIGLGALAMLEDGLYERFPKPDYVLGFHDAAQFPAGMIGYSPGYALANVDSVDITVKGVGGHGAYPHTAKDPIV
LASAIVMKLQTLVSRESSPLDPAVVTVGSFLAGAKHNIIPDEAKLQLTVRSYSDESRQLLLDGIKRIVKGEAMAAGMPDD
RMPEVNVILPYTPSTYNTPEFTEQVMAKLTDRFGEGRVMQVPSVMGGEDFGQFYRADRENIESLIFWVGGVPQDEFDAAQ
RGETKLPSLHSPFWAPDAEKVIATATEAMVASTLDLMSASGG

Sequences:

>Translated_442_residues
MNWVLGLAAVSALALVASAASANELREDVMGDMPGLVELYKDLHANPELSFQEFETAKKLADRARALGFEVTEGVGQTGV
VAVMRNGEGPTVMLRADMDGLPVVEQTGLPYASKRTAVPASGVETGVMHACGHDTHMAAWVGAAQLLSERKAEWSGTLVM
ILQPAEEIGLGALAMLEDGLYERFPKPDYVLGFHDAAQFPAGMIGYSPGYALANVDSVDITVKGVGGHGAYPHTAKDPIV
LASAIVMKLQTLVSRESSPLDPAVVTVGSFLAGAKHNIIPDEAKLQLTVRSYSDESRQLLLDGIKRIVKGEAMAAGMPDD
RMPEVNVILPYTPSTYNTPEFTEQVMAKLTDRFGEGRVMQVPSVMGGEDFGQFYRADRENIESLIFWVGGVPQDEFDAAQ
RGETKLPSLHSPFWAPDAEKVIATATEAMVASTLDLMSASGG
>Mature_442_residues
MNWVLGLAAVSALALVASAASANELREDVMGDMPGLVELYKDLHANPELSFQEFETAKKLADRARALGFEVTEGVGQTGV
VAVMRNGEGPTVMLRADMDGLPVVEQTGLPYASKRTAVPASGVETGVMHACGHDTHMAAWVGAAQLLSERKAEWSGTLVM
ILQPAEEIGLGALAMLEDGLYERFPKPDYVLGFHDAAQFPAGMIGYSPGYALANVDSVDITVKGVGGHGAYPHTAKDPIV
LASAIVMKLQTLVSRESSPLDPAVVTVGSFLAGAKHNIIPDEAKLQLTVRSYSDESRQLLLDGIKRIVKGEAMAAGMPDD
RMPEVNVILPYTPSTYNTPEFTEQVMAKLTDRFGEGRVMQVPSVMGGEDFGQFYRADRENIESLIFWVGGVPQDEFDAAQ
RGETKLPSLHSPFWAPDAEKVIATATEAMVASTLDLMSASGG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=325, Percent_Identity=26.1538461538462, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 47210; Mature: 47210

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWVLGLAAVSALALVASAASANELREDVMGDMPGLVELYKDLHANPELSFQEFETAKKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ADRARALGFEVTEGVGQTGVVAVMRNGEGPTVMLRADMDGLPVVEQTGLPYASKRTAVPA
HHHHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCC
SGVETGVMHACGHDTHMAAWVGAAQLLSERKAEWSGTLVMILQPAEEIGLGALAMLEDGL
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHH
YERFPKPDYVLGFHDAAQFPAGMIGYSPGYALANVDSVDITVKGVGGHGAYPHTAKDPIV
HHHCCCCCEEEEECHHHHCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCHHH
LASAIVMKLQTLVSRESSPLDPAVVTVGSFLAGAKHNIIPDEAKLQLTVRSYSDESRQLL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHH
LDGIKRIVKGEAMAAGMPDDRMPEVNVILPYTPSTYNTPEFTEQVMAKLTDRFGEGRVMQ
HHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
VPSVMGGEDFGQFYRADRENIESLIFWVGGVPQDEFDAAQRGETKLPSLHSPFWAPDAEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHH
VIATATEAMVASTLDLMSASGG
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNWVLGLAAVSALALVASAASANELREDVMGDMPGLVELYKDLHANPELSFQEFETAKKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ADRARALGFEVTEGVGQTGVVAVMRNGEGPTVMLRADMDGLPVVEQTGLPYASKRTAVPA
HHHHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCC
SGVETGVMHACGHDTHMAAWVGAAQLLSERKAEWSGTLVMILQPAEEIGLGALAMLEDGL
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHH
YERFPKPDYVLGFHDAAQFPAGMIGYSPGYALANVDSVDITVKGVGGHGAYPHTAKDPIV
HHHCCCCCEEEEECHHHHCCCCCCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCCHHH
LASAIVMKLQTLVSRESSPLDPAVVTVGSFLAGAKHNIIPDEAKLQLTVRSYSDESRQLL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHH
LDGIKRIVKGEAMAAGMPDDRMPEVNVILPYTPSTYNTPEFTEQVMAKLTDRFGEGRVMQ
HHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
VPSVMGGEDFGQFYRADRENIESLIFWVGGVPQDEFDAAQRGETKLPSLHSPFWAPDAEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHH
VIATATEAMVASTLDLMSASGG
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]