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The map label for this gene is menB [C]

Identifier: 85373521

GI number: 85373521

Start: 716260

End: 717093

Strand: Direct

Name: menB [C]

Synonym: ELI_03470

Alternate gene names: 85373521

Gene position: 716260-717093 (Clockwise)

Preceding gene: 85373520

Following gene: 85373522

Centisome position: 23.47

GC content: 66.31

Gene sequence:

>834_bases
ATGACCGATCAACGCGTCCGAACCGATAATCCCGCAAATGGCGTCGCCCGCCTCACCCTGACGCGCGCCGACAAGGCCAA
TGCGCAGGACAAGGCGATGCTGTACCAGCTCGACGATGCGCTGACCGCAGCGGCGCAGAATGACGATGTGCGCTGCATCG
TCCTGGCGGCGGAAGGCGAGCACTTCTCCTCCGGTCACGACCTGCGCGACGACAGCTCGCTCGCAGACTTCGAACCGCGC
AGCCAGTGGAGCGGCTTCGATGCATCGGGGCAGGAAGGCCAGATGGCCGCCGAGCACGAGATCTATCTCGGCCTGTGCTG
GCGCTGGCGCAACCTGCCCAAGCCGGTGGTGGTGCAGGTGCAGGGCCTGTGCATGGCAGGCGGGCTGATGCTGGTCTGGC
CCTTCGACATCGTGATCGCGAGCGAGGATGCGGTGTTCTCCGATCCGACGGTGGCGTTCGGCGTCAACGGGGTCGAATAT
TTCGCGCATGTCTGGGAGCTCGGCCACCGCAAGGCGCGCGAACTGCTCTACACCGGCGCGGACCTGAGCGCCGAGGATGC
GCGGACGCTTGGCATGGTCAACCACGTCGTGCCGCGCGACGAGTTGGATAGCTTTACACTGACGATGGCCGAGCGGATCG
CGAAGCGTCCGATGATCGGGCTGCGGCTGGCCAAGCTGGCCTGCAACCAGAGCCTCGATGCGCAGGGCTATGGGACGGCT
ATCCATGGCGTGATGGGTTGGCAGCAGGTCGGCCACGCGCAGGCGCGGCTGGTGCACGGGTTGCCGGTCGATCCGGCGGG
CGCGGAGATTATCCGCTCGGATCGCAAGCGATAG

Upstream 100 bases:

>100_bases
TGTTGCGAGACCCGAAGGTCGCGCGCGATCTGGGCGACGAGGAAGAGTAGCGCGCCGCACTCGCTTTGTTGCTAGGCTCT
TCGCAAGGAAGAGGAGAGCC

Downstream 100 bases:

>100_bases
GTGCTTGTCGGCTCTCGCGCTGGCTGCGATGACTGGCGCGAGAGGAGACCCGCATGACCGAAGCCCTGCGTACCCCAGAC
GAAGCCTTCGCCGAAGTCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTDQRVRTDNPANGVARLTLTRADKANAQDKAMLYQLDDALTAAAQNDDVRCIVLAAEGEHFSSGHDLRDDSSLADFEPR
SQWSGFDASGQEGQMAAEHEIYLGLCWRWRNLPKPVVVQVQGLCMAGGLMLVWPFDIVIASEDAVFSDPTVAFGVNGVEY
FAHVWELGHRKARELLYTGADLSAEDARTLGMVNHVVPRDELDSFTLTMAERIAKRPMIGLRLAKLACNQSLDAQGYGTA
IHGVMGWQQVGHAQARLVHGLPVDPAGAEIIRSDRKR

Sequences:

>Translated_277_residues
MTDQRVRTDNPANGVARLTLTRADKANAQDKAMLYQLDDALTAAAQNDDVRCIVLAAEGEHFSSGHDLRDDSSLADFEPR
SQWSGFDASGQEGQMAAEHEIYLGLCWRWRNLPKPVVVQVQGLCMAGGLMLVWPFDIVIASEDAVFSDPTVAFGVNGVEY
FAHVWELGHRKARELLYTGADLSAEDARTLGMVNHVVPRDELDSFTLTMAERIAKRPMIGLRLAKLACNQSLDAQGYGTA
IHGVMGWQQVGHAQARLVHGLPVDPAGAEIIRSDRKR
>Mature_276_residues
TDQRVRTDNPANGVARLTLTRADKANAQDKAMLYQLDDALTAAAQNDDVRCIVLAAEGEHFSSGHDLRDDSSLADFEPRS
QWSGFDASGQEGQMAAEHEIYLGLCWRWRNLPKPVVVQVQGLCMAGGLMLVWPFDIVIASEDAVFSDPTVAFGVNGVEYF
AHVWELGHRKARELLYTGADLSAEDARTLGMVNHVVPRDELDSFTLTMAERIAKRPMIGLRLAKLACNQSLDAQGYGTAI
HGVMGWQQVGHAQARLVHGLPVDPAGAEIIRSDRKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=212, Percent_Identity=31.1320754716981, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=26.9767441860465, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=26.9767441860465, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=26.9767441860465, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194097323, Length=225, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=236, Percent_Identity=29.2372881355932, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082183, Length=217, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787659, Length=227, Percent_Identity=25.9911894273128, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787660, Length=233, Percent_Identity=31.7596566523605, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=28.5, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17540714, Length=266, Percent_Identity=27.4436090225564, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=226, Percent_Identity=24.7787610619469, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=24.4979919678715, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=214, Percent_Identity=25.2336448598131, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=206, Percent_Identity=28.1553398058252, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=24.0909090909091, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19920382, Length=234, Percent_Identity=29.0598290598291, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=239, Percent_Identity=24.6861924686192, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653477, Length=239, Percent_Identity=24.6861924686192, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653139, Length=244, Percent_Identity=26.6393442622951, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30409; Mature: 30278

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQRVRTDNPANGVARLTLTRADKANAQDKAMLYQLDDALTAAAQNDDVRCIVLAAEGE
CCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHEEEHHHHHHHHCCCCCEEEEEEEECCC
HFSSGHDLRDDSSLADFEPRSQWSGFDASGQEGQMAAEHEIYLGLCWRWRNLPKPVVVQV
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEECCCEEEEEEEEHHCCCCCCEEEEE
QGLCMAGGLMLVWPFDIVIASEDAVFSDPTVAFGVNGVEYFAHVWELGHRKARELLYTGA
ECCHHHCCEEEEEEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
DLSAEDARTLGMVNHVVPRDELDSFTLTMAERIAKRPMIGLRLAKLACNQSLDAQGYGTA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
IHGVMGWQQVGHAQARLVHGLPVDPAGAEIIRSDRKR
HHHHHHHHHHCHHHHHEECCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TDQRVRTDNPANGVARLTLTRADKANAQDKAMLYQLDDALTAAAQNDDVRCIVLAAEGE
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHEEEHHHHHHHHCCCCCEEEEEEEECCC
HFSSGHDLRDDSSLADFEPRSQWSGFDASGQEGQMAAEHEIYLGLCWRWRNLPKPVVVQV
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEECCCEEEEEEEEHHCCCCCCEEEEE
QGLCMAGGLMLVWPFDIVIASEDAVFSDPTVAFGVNGVEYFAHVWELGHRKARELLYTGA
ECCHHHCCEEEEEEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
DLSAEDARTLGMVNHVVPRDELDSFTLTMAERIAKRPMIGLRLAKLACNQSLDAQGYGTA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
IHGVMGWQQVGHAQARLVHGLPVDPAGAEIIRSDRKR
HHHHHHHHHHCHHHHHEECCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA