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The map label for this gene is glk [H]

Identifier: 85372934

GI number: 85372934

Start: 138241

End: 139212

Strand: Reverse

Name: glk [H]

Synonym: ELI_00535

Alternate gene names: 85372934

Gene position: 139212-138241 (Counterclockwise)

Preceding gene: 85372935

Following gene: 85372933

Centisome position: 4.56

GC content: 66.15

Gene sequence:

>972_bases
ATGGACCTCGTCGCCGTCGATATCGGGGGAACCCACGCCCGCTTCGCCATTGCCACTGTCGCGGAGGACGGCGCGATCAC
GCTCGGCGAACCGGAGACGCTCCACACCGAAGATCACGCCAGCTTCCAGACCGCGTGGGAGGATTTTCGCGATCGCATGG
GCGGCAGCCTGCCCGACGCGATCAGCATGGCCGTCGCCGGGCCGGTCAAGACCGACATCATCCGCTTCACCAACAATCCC
TGGATCATCCGTCCGCCGCTGATTGCCGAAAAGCTCGGTTGCGGGCGCTACACCATCGTCAACGATTTCGAAGCCGTGGC
GCATGCTGCGGCCCGCGCACCAGCGGAACAGTTTATCCATCTCGCAGGGCCGGAGCAGGACCTGCCCGCTAAAGGCACCA
TCAGCGTGCTTGGCCCTGGCACCGGACTAGGCGTCGCTCATTTCTGGCGCGACGGAGACGGCGGCTACCGCGTGCAGGCG
ACCGAGGGCGGGCATGGCGATTTCGCCCCGCTCGATTCCATCGAGGATGCGATCCTCGCCCGCCTGCGCAAGCGGCACAT
GCGCGTGTCGGACGAACGCGTAGTTTCGGGACCGGCCATCGTCGACATCTACCACACGCTCGCTGCCATGGAGGGCCGCG
CGGTGCAGGAGCTCGACGATGTCGAGATCTGGACCCGCGGTACCAGCGGCGACGACAGCCTCGCCGCCGCCGCTGTCGAC
CGCTTCTGTCTCGCTCTCGGCAGTGTCGCGGGCGATATCGCGTTGATCCAAGGCGCGAGCGGCGTCGTGATAGCAGGTGG
CCTCGGCTATCGCATCCGTGAGACACTGACCGCTTCCGGTTTCGAGGACCGCTTTCGCGCCAAGGGACGCTTTGCCGGCC
TGATGGCGACGCTGCCGGTCAAGCTGATCGTCCATCCCCAACCCGGCCTGTTCGGCGCGGCCGCAGCCTTTGCAAGGGAA
CATCTATCGTGA

Upstream 100 bases:

>100_bases
CCGATTGGGGCGTAGGCCGCGAGATGTTCAGCATGTTCCGAATGCACGCCGACGGGGCCGAACAAGGTGCCAGTCCGATG
TTGGCTGCGGCGGGTCTTTG

Downstream 100 bases:

>100_bases
GCACTATCGAATCCATCATGCGCACGGCGCCCGTGATCCCGGTGATCGTGATCGATGACGTGGCCGATGCGGTTCCGCTC
GCCGAAGCCTTGGTCGAAGG

Product: glucokinase

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDLVAVDIGGTHARFAIATVAEDGAITLGEPETLHTEDHASFQTAWEDFRDRMGGSLPDAISMAVAGPVKTDIIRFTNNP
WIIRPPLIAEKLGCGRYTIVNDFEAVAHAAARAPAEQFIHLAGPEQDLPAKGTISVLGPGTGLGVAHFWRDGDGGYRVQA
TEGGHGDFAPLDSIEDAILARLRKRHMRVSDERVVSGPAIVDIYHTLAAMEGRAVQELDDVEIWTRGTSGDDSLAAAAVD
RFCLALGSVAGDIALIQGASGVVIAGGLGYRIRETLTASGFEDRFRAKGRFAGLMATLPVKLIVHPQPGLFGAAAAFARE
HLS

Sequences:

>Translated_323_residues
MDLVAVDIGGTHARFAIATVAEDGAITLGEPETLHTEDHASFQTAWEDFRDRMGGSLPDAISMAVAGPVKTDIIRFTNNP
WIIRPPLIAEKLGCGRYTIVNDFEAVAHAAARAPAEQFIHLAGPEQDLPAKGTISVLGPGTGLGVAHFWRDGDGGYRVQA
TEGGHGDFAPLDSIEDAILARLRKRHMRVSDERVVSGPAIVDIYHTLAAMEGRAVQELDDVEIWTRGTSGDDSLAAAAVD
RFCLALGSVAGDIALIQGASGVVIAGGLGYRIRETLTASGFEDRFRAKGRFAGLMATLPVKLIVHPQPGLFGAAAAFARE
HLS
>Mature_323_residues
MDLVAVDIGGTHARFAIATVAEDGAITLGEPETLHTEDHASFQTAWEDFRDRMGGSLPDAISMAVAGPVKTDIIRFTNNP
WIIRPPLIAEKLGCGRYTIVNDFEAVAHAAARAPAEQFIHLAGPEQDLPAKGTISVLGPGTGLGVAHFWRDGDGGYRVQA
TEGGHGDFAPLDSIEDAILARLRKRHMRVSDERVVSGPAIVDIYHTLAAMEGRAVQELDDVEIWTRGTSGDDSLAAAAVD
RFCLALGSVAGDIALIQGASGVVIAGGLGYRIRETLTASGFEDRFRAKGRFAGLMATLPVKLIVHPQPGLFGAAAAFARE
HLS

Specific function: Not Highly Important In E.Coli As Glucose Is Transported Into The Cell By The Pts System Already As Glucose 6-Phosphate. [C]

COG id: COG0837

COG function: function code G; Glucokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial glucokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788732, Length=311, Percent_Identity=35.6913183279743, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003836 [H]

Pfam domain/function: PF02685 Glucokinase [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 34209; Mature: 34209

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVAVDIGGTHARFAIATVAEDGAITLGEPETLHTEDHASFQTAWEDFRDRMGGSLPDA
CCEEEEECCCCCEEEEEEEEECCCEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCCCHH
ISMAVAGPVKTDIIRFTNNPWIIRPPLIAEKLGCGRYTIVNDFEAVAHAAARAPAEQFIH
HHHHHCCCCCEEEEEECCCCEEEECCHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHEEE
LAGPEQDLPAKGTISVLGPGTGLGVAHFWRDGDGGYRVQATEGGHGDFAPLDSIEDAILA
CCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHH
RLRKRHMRVSDERVVSGPAIVDIYHTLAAMEGRAVQELDDVEIWTRGTSGDDSLAAAAVD
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCHHHHHHHHH
RFCLALGSVAGDIALIQGASGVVIAGGLGYRIRETLTASGFEDRFRAKGRFAGLMATLPV
HHHHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHCCCCHHHHHHCCHHHHHHEECCE
KLIVHPQPGLFGAAAAFAREHLS
EEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDLVAVDIGGTHARFAIATVAEDGAITLGEPETLHTEDHASFQTAWEDFRDRMGGSLPDA
CCEEEEECCCCCEEEEEEEEECCCEEECCCCCEECCCCCHHHHHHHHHHHHHHCCCCCHH
ISMAVAGPVKTDIIRFTNNPWIIRPPLIAEKLGCGRYTIVNDFEAVAHAAARAPAEQFIH
HHHHHCCCCCEEEEEECCCCEEEECCHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHEEE
LAGPEQDLPAKGTISVLGPGTGLGVAHFWRDGDGGYRVQATEGGHGDFAPLDSIEDAILA
CCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHH
RLRKRHMRVSDERVVSGPAIVDIYHTLAAMEGRAVQELDDVEIWTRGTSGDDSLAAAAVD
HHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCHHHHHHHHH
RFCLALGSVAGDIALIQGASGVVIAGGLGYRIRETLTASGFEDRFRAKGRFAGLMATLPV
HHHHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHCCCCHHHHHHCCHHHHHHEECCE
KLIVHPQPGLFGAAAAFAREHLS
EEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254282 [H]