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The map label for this gene is eda [H]

Identifier: 85372933

GI number: 85372933

Start: 137630

End: 138244

Strand: Reverse

Name: eda [H]

Synonym: ELI_00530

Alternate gene names: 85372933

Gene position: 138244-137630 (Counterclockwise)

Preceding gene: 85372934

Following gene: 85372932

Centisome position: 4.53

GC content: 66.67

Gene sequence:

>615_bases
GTGAGCACTATCGAATCCATCATGCGCACGGCGCCCGTGATCCCGGTGATCGTGATCGATGACGTGGCCGATGCGGTTCC
GCTCGCCGAAGCCTTGGTCGAAGGCGGCTTGCGCGTGCTTGAAGTGACCCTGCGCACCCCCGCTGCGCTCGATGCGATCA
GGGCCATGAAGCAGGTCGCAGGGGCGATCGTCGGCGCTGGGACTGTCATCAACCAGCAGGACCTGGCCGACGCGCTCGAT
GCAGGCAGCGAATTCATCGTCTCGCCGGGGCTCACCGAGCCGCTCGGCAAGGCCGCGATCCGCGCCGAGGTGCCGTTTCT
TCCCGGTATCGCGAATGCGGGCGACATCATGCGCGGGCTGGACCTTGGCCTCACGCACTTCAAGTTCTTCCCCGCCATGG
CCGCAGGGGGCCTGCCCGCCCTGAAAGCGCTCGCTGCGCCTTTCGGCCAGTGCAAGTTCTGCCCCACAGGCGGCATCAGC
GAGGATACGGCACCCGAATGGCTCGCTTTCGATCCGATCCTGTGCGTTGGCGGCAGCTGGGTCGCGCCGCGCCATCCGAG
CGATTTCGCCGCCGTCACACAATCGGCACAGGCTGCCGCCAAGCTCACGCCATGA

Upstream 100 bases:

>100_bases
GCTTTGCCGGCCTGATGGCGACGCTGCCGGTCAAGCTGATCGTCCATCCCCAACCCGGCCTGTTCGGCGCGGCCGCAGCC
TTTGCAAGGGAACATCTATC

Downstream 100 bases:

>100_bases
AGTCCGTCACCGACCTTCGCGAGCGCCTGCAGGACATGCACAGCCGGACAGCGGAAACCCCGCTGTTCAACCCGGTGTTC
CAGCTATCGCTCGACCTTTC

Product: KDPG aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSTIESIMRTAPVIPVIVIDDVADAVPLAEALVEGGLRVLEVTLRTPAALDAIRAMKQVAGAIVGAGTVINQQDLADALD
AGSEFIVSPGLTEPLGKAAIRAEVPFLPGIANAGDIMRGLDLGLTHFKFFPAMAAGGLPALKALAAPFGQCKFCPTGGIS
EDTAPEWLAFDPILCVGGSWVAPRHPSDFAAVTQSAQAAAKLTP

Sequences:

>Translated_204_residues
MSTIESIMRTAPVIPVIVIDDVADAVPLAEALVEGGLRVLEVTLRTPAALDAIRAMKQVAGAIVGAGTVINQQDLADALD
AGSEFIVSPGLTEPLGKAAIRAEVPFLPGIANAGDIMRGLDLGLTHFKFFPAMAAGGLPALKALAAPFGQCKFCPTGGIS
EDTAPEWLAFDPILCVGGSWVAPRHPSDFAAVTQSAQAAAKLTP
>Mature_203_residues
STIESIMRTAPVIPVIVIDDVADAVPLAEALVEGGLRVLEVTLRTPAALDAIRAMKQVAGAIVGAGTVINQQDLADALDA
GSEFIVSPGLTEPLGKAAIRAEVPFLPGIANAGDIMRGLDLGLTHFKFFPAMAAGGLPALKALAAPFGQCKFCPTGGISE
DTAPEWLAFDPILCVGGSWVAPRHPSDFAAVTQSAQAAAKLTP

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=204, Percent_Identity=52.9411764705882, Blast_Score=197, Evalue=6e-52,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 20893; Mature: 20762

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIESIMRTAPVIPVIVIDDVADAVPLAEALVEGGLRVLEVTLRTPAALDAIRAMKQVA
CCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHH
GAIVGAGTVINQQDLADALDAGSEFIVSPGLTEPLGKAAIRAEVPFLPGIANAGDIMRGL
HHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
DLGLTHFKFFPAMAAGGLPALKALAAPFGQCKFCPTGGISEDTAPEWLAFDPILCVGGSW
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCC
VAPRHPSDFAAVTQSAQAAAKLTP
CCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STIESIMRTAPVIPVIVIDDVADAVPLAEALVEGGLRVLEVTLRTPAALDAIRAMKQVA
CHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHH
GAIVGAGTVINQQDLADALDAGSEFIVSPGLTEPLGKAAIRAEVPFLPGIANAGDIMRGL
HHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
DLGLTHFKFFPAMAAGGLPALKALAAPFGQCKFCPTGGISEDTAPEWLAFDPILCVGGSW
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCC
VAPRHPSDFAAVTQSAQAAAKLTP
CCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1809834 [H]