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The map label for this gene is ilvA [H]

Identifier: 85060371

GI number: 85060371

Start: 4101139

End: 4102683

Strand: Reverse

Name: ilvA [H]

Synonym: SG2393

Alternate gene names: 85060371

Gene position: 4102683-4101139 (Counterclockwise)

Preceding gene: 85060372

Following gene: 85060369

Centisome position: 98.36

GC content: 60.97

Gene sequence:

>1545_bases
ATGGCTGAGTCTCAACCGTTTTCCGCCGAGCCCTGCCCTGCGGAGTACCTGCGCGCGGCGTTACGCGCGCAGGTGTATGA
AGTGGCCCAGGTGACACCGCTGCAGGTGATGAATAAAGTGTCGGAACGCCTCGGCAATACCGTACTGGTGAAACGTGAAG
ACCGTCAGCCGGTGCACAGCTTTAAACTGCGGGGCGCTTACGCGATGATTGAGGGGTTCAGCGAAGCACAGCGCGCGTGC
GGCGTCATTACCGCCTCGGCGGGCAACCATGCCCAAGGGGTGGCGTTGTCCGCCAGCCAGCTCGGCATTCCGTCGCTTAT
TGTCATGCCGACCAACACCGCCGATATCAAAGTGGATGCGGTGCGCGGCTTCGGCGGCGAGCCGCTGTTGTTCGGCGCCA
ACTTTGATGAAGCTAAGGCCAAAGCCATTGCGCTGGCGCGCGAACGCCATATGACTTTTGTGCCGCCGTTCGATCATCCT
GCGGTGATCGCCGGGCAGGGGACGCTGGCGATGGAGCTGCTTCAGCAAGACGCCCATCTGGACCGCATATTTGTGCCGGT
GGGCGGCGGCGGTCTGGCGGCGGGCGTGGCGGTGTTGATAAAGCATCTGATGCCACAGATCAAAGTCATCGGCGTCGAAG
CGGAAGAGTCCGCCTGTCTGGCGGCGGCGCTACACGCCGGCGAGCCGGTGGATTTACAGCGGGTCGGTCTGTTTGCTGAG
GGGGTGGCGGTAAGGCGTATCGGCGATGAAACCTTCCGTCTTTGCCGCGATTACCTTGATGATGTGGTCACCGTGGACAG
CGACGCGATTTGCGCGGCGGTAAAAGATTTGTTTGAGGACGTACGGGCGATTGCCGAACCCTCCGGCGCGCTGGCGCTGG
CGGGGATGAAAAAATATGTCCAACAACACGGGATCCGGGATGAGCGTCTGGCGCACGTACTGTCCGGCGCTAATGTCAAT
TTCCATGGCCTGCGCTATGTCTCGGAGCGCTGCGAGCTGGGCGAGCAACGCGAGGCGCTGATGGCGGTGACCATTCCGGA
AAGGAAGGGCAGTTTTCTGGCCTTCTGCGAGCTCCTAGGCGGTCGGCCGGTAACGGAGTTCAATTATCGCTACAGCGATG
CCGACAACGCCTGTATTTTCGTCGGCGTCAGGCTGACGCGCGGGTGCGAGGAGCGCGAGGAAATCCTGGCGGAGATGGTT
GCCGGCGGCTATCAGGTGGCGGATCTATCCGACGACGAAATGGCCAAGCTGCATGTCCGCTATATGGTGGGTGGGCGACC
GTCGAAACCGCTGCGCGAGCGTTTGTACAGCTTCGAGTTCCCCGAATCCCCGGGAGCATTGCTGAAATTCCTCTACACGC
TGGGCACGCGCTGGAATATCACCTTATTCCACTACCGCAGTCACGGCACGGATTTCGGCCGGGTCTTGGCGGGCTTTGAA
CTGGCGGAGCGTGAGCCGGAGTTCGATCATCACCTGTTGGCATTGGGCTATGAATGCCACGATGTCACCGCCAATCCGGC
GTTTCGTTTGTTCCTGGCGGATTAA

Upstream 100 bases:

>100_bases
ACGGCAGGTGTCGTTCGCCCTGAAAGCCTATGCCAGTTTGGCGACCAGTGCAGACAAAGGCGCGGTGCGCGATAAAGCCA
AGTTGGGAGGATAAGGCACC

Downstream 100 bases:

>100_bases
CCGCGCTGGCGGCCGGGGCGAGTGCATCTTCTGGCGCCGGTCAGCGTTCCAGCAGTTGCCAGAAGGCGTCGATCAGCGGT
TCATGTAGCCGCTTTATTTG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MAESQPFSAEPCPAEYLRAALRAQVYEVAQVTPLQVMNKVSERLGNTVLVKREDRQPVHSFKLRGAYAMIEGFSEAQRAC
GVITASAGNHAQGVALSASQLGIPSLIVMPTNTADIKVDAVRGFGGEPLLFGANFDEAKAKAIALARERHMTFVPPFDHP
AVIAGQGTLAMELLQQDAHLDRIFVPVGGGGLAAGVAVLIKHLMPQIKVIGVEAEESACLAAALHAGEPVDLQRVGLFAE
GVAVRRIGDETFRLCRDYLDDVVTVDSDAICAAVKDLFEDVRAIAEPSGALALAGMKKYVQQHGIRDERLAHVLSGANVN
FHGLRYVSERCELGEQREALMAVTIPERKGSFLAFCELLGGRPVTEFNYRYSDADNACIFVGVRLTRGCEEREEILAEMV
AGGYQVADLSDDEMAKLHVRYMVGGRPSKPLRERLYSFEFPESPGALLKFLYTLGTRWNITLFHYRSHGTDFGRVLAGFE
LAEREPEFDHHLLALGYECHDVTANPAFRLFLAD

Sequences:

>Translated_514_residues
MAESQPFSAEPCPAEYLRAALRAQVYEVAQVTPLQVMNKVSERLGNTVLVKREDRQPVHSFKLRGAYAMIEGFSEAQRAC
GVITASAGNHAQGVALSASQLGIPSLIVMPTNTADIKVDAVRGFGGEPLLFGANFDEAKAKAIALARERHMTFVPPFDHP
AVIAGQGTLAMELLQQDAHLDRIFVPVGGGGLAAGVAVLIKHLMPQIKVIGVEAEESACLAAALHAGEPVDLQRVGLFAE
GVAVRRIGDETFRLCRDYLDDVVTVDSDAICAAVKDLFEDVRAIAEPSGALALAGMKKYVQQHGIRDERLAHVLSGANVN
FHGLRYVSERCELGEQREALMAVTIPERKGSFLAFCELLGGRPVTEFNYRYSDADNACIFVGVRLTRGCEEREEILAEMV
AGGYQVADLSDDEMAKLHVRYMVGGRPSKPLRERLYSFEFPESPGALLKFLYTLGTRWNITLFHYRSHGTDFGRVLAGFE
LAEREPEFDHHLLALGYECHDVTANPAFRLFLAD
>Mature_513_residues
AESQPFSAEPCPAEYLRAALRAQVYEVAQVTPLQVMNKVSERLGNTVLVKREDRQPVHSFKLRGAYAMIEGFSEAQRACG
VITASAGNHAQGVALSASQLGIPSLIVMPTNTADIKVDAVRGFGGEPLLFGANFDEAKAKAIALARERHMTFVPPFDHPA
VIAGQGTLAMELLQQDAHLDRIFVPVGGGGLAAGVAVLIKHLMPQIKVIGVEAEESACLAAALHAGEPVDLQRVGLFAEG
VAVRRIGDETFRLCRDYLDDVVTVDSDAICAAVKDLFEDVRAIAEPSGALALAGMKKYVQQHGIRDERLAHVLSGANVNF
HGLRYVSERCELGEQREALMAVTIPERKGSFLAFCELLGGRPVTEFNYRYSDADNACIFVGVRLTRGCEEREEILAEMVA
GGYQVADLSDDEMAKLHVRYMVGGRPSKPLRERLYSFEFPESPGALLKFLYTLGTRWNITLFHYRSHGTDFGRVLAGFEL
AEREPEFDHHLLALGYECHDVTANPAFRLFLAD

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=297, Percent_Identity=31.986531986532, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923959, Length=309, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI33469958, Length=315, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790207, Length=513, Percent_Identity=79.9220272904483, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=291, Percent_Identity=36.7697594501718, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1789235, Length=273, Percent_Identity=25.6410256410256, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=372, Percent_Identity=30.6451612903226, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537387, Length=314, Percent_Identity=32.1656050955414, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17508781, Length=286, Percent_Identity=30.7692307692308, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320930, Length=514, Percent_Identity=48.443579766537, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322631, Length=318, Percent_Identity=34.2767295597484, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6319788, Length=266, Percent_Identity=27.4436090225564, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=31.0344827586207, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24645328, Length=251, Percent_Identity=29.0836653386454, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56200; Mature: 56069

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESQPFSAEPCPAEYLRAALRAQVYEVAQVTPLQVMNKVSERLGNTVLVKREDRQPVHS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCHHH
FKLRGAYAMIEGFSEAQRACGVITASAGNHAQGVALSASQLGIPSLIVMPTNTADIKVDA
HHHCCHHHHHHCHHHHHHHCEEEEECCCCCCCCEEEEHHHCCCCEEEEEECCCCCEEEEE
VRGFGGEPLLFGANFDEAKAKAIALARERHMTFVPPFDHPAVIAGQGTLAMELLQQDAHL
EECCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCC
DRIFVPVGGGGLAAGVAVLIKHLMPQIKVIGVEAEESACLAAALHAGEPVDLQRVGLFAE
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHC
GVAVRRIGDETFRLCRDYLDDVVTVDSDAICAAVKDLFEDVRAIAEPSGALALAGMKKYV
CCHHHHCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
QQHGIRDERLAHVLSGANVNFHGLRYVSERCELGEQREALMAVTIPERKGSFLAFCELLG
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHC
GRPVTEFNYRYSDADNACIFVGVRLTRGCEEREEILAEMVAGGYQVADLSDDEMAKLHVR
CCCCEECCEEECCCCCCEEEEEEEHHCCCHHHHHHHHHHHHCCEEEECCCCCHHHEEEEE
YMVGGRPSKPLRERLYSFEFPESPGALLKFLYTLGTRWNITLFHYRSHGTDFGRVLAGFE
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHCCH
LAEREPEFDHHLLALGYECHDVTANPAFRLFLAD
HHCCCCCCCCEEEEECCEEEECCCCCEEEEEEEC
>Mature Secondary Structure 
AESQPFSAEPCPAEYLRAALRAQVYEVAQVTPLQVMNKVSERLGNTVLVKREDRQPVHS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCHHH
FKLRGAYAMIEGFSEAQRACGVITASAGNHAQGVALSASQLGIPSLIVMPTNTADIKVDA
HHHCCHHHHHHCHHHHHHHCEEEEECCCCCCCCEEEEHHHCCCCEEEEEECCCCCEEEEE
VRGFGGEPLLFGANFDEAKAKAIALARERHMTFVPPFDHPAVIAGQGTLAMELLQQDAHL
EECCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCC
DRIFVPVGGGGLAAGVAVLIKHLMPQIKVIGVEAEESACLAAALHAGEPVDLQRVGLFAE
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHC
GVAVRRIGDETFRLCRDYLDDVVTVDSDAICAAVKDLFEDVRAIAEPSGALALAGMKKYV
CCHHHHCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHH
QQHGIRDERLAHVLSGANVNFHGLRYVSERCELGEQREALMAVTIPERKGSFLAFCELLG
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHC
GRPVTEFNYRYSDADNACIFVGVRLTRGCEEREEILAEMVAGGYQVADLSDDEMAKLHVR
CCCCEECCEEECCCCCCEEEEEEEHHCCCHHHHHHHHHHHHCCEEEECCCCCHHHEEEEE
YMVGGRPSKPLRERLYSFEFPESPGALLKFLYTLGTRWNITLFHYRSHGTDFGRVLAGFE
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHCCH
LAEREPEFDHHLLALGYECHDVTANPAFRLFLAD
HHCCCCCCCCEEEEECCEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]