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The map label for this gene is uvrD

Identifier: 85060320

GI number: 85060320

Start: 4019657

End: 4021819

Strand: Reverse

Name: uvrD

Synonym: SG2342

Alternate gene names: 85060320

Gene position: 4021819-4019657 (Counterclockwise)

Preceding gene: 85060321

Following gene: 85060319

Centisome position: 96.42

GC content: 60.61

Gene sequence:

>2163_bases
ATGGACGTTTCTGATTTGCTCAACAGCTTGAACGACAAGCAACGGGAAGCCGTGGTGGCGCCGCGCGGTAATTTGCTGGT
GCTGGCCGGCGCCGGCAGCGGCAAAACGCGGGTGCTGGTGCATCGTATCGCCTGGCTGCTGTCGGTGGAAAATTGCTCGC
CCTATTCGGTAATGGCGGTCACCTTCACCAACAAGGCGGCGGCGGAGATGCGCCACCGCATCGAACATCTGGTGGGCACC
AGCCAGGGCGGCATGTGGATCGGTACCTTCCATGGGCTGGCCCATCGCCTGTTGCGTGCCCACCATCAGGATGCCAACCT
GCCGCAGGATTTTCAGATCCTCGATAGTGAAGATCAACTGCGCTTGTTAAAACGCCTTATCCGCGCCATGAATCTGGATG
AGAAACAGTGGCCGCCGCGCCAGGCGATGTGGTATATCGGCGGCAAAAAAGACGATGGACTGCGTCCGCAGCATGTGGAG
AGTTACGGCAATCCGGTCGAGGCGACCTGGCAGCGCATCTATCAGGCCTATCAGGAAGCGTGCGACCGCGCCGGGCTGGT
GGATTTTGCCGAGCTGCTGCTGCGCGCCCACGAGCTGTGGCTAAACAAGCCGCATATTCTGCGCCACTACCGCGAACGTT
TTACCAATATCCTGGTGGATGAATTCCAGGATACCAACCAAATTCAATACGCCTGGATCCGCATGCTGGCCGGCGATGAC
GGCAATGTGATGATCGTCGGCGACGATGACCAGTCGATCTACGGCTGGCGCGGGGCGCAGGTAGAAAACATCCAGCGCTT
CCTGGACGATTTCCCCGGCGCGCAGACCATTCGATTGGAGCAAAATTACCGCTCCACCAGCAATATCCTGCAGGCGGCCA
ACGCCCTTATCGCCCACAACGGCGGCCGGCTGGGCAAAAATCTGTGGACCGAGGGCGCTGAAGGCGAACCGATTACCCTT
TACTGCGCCTTTAACGAACTGGACGAGGCGCTCTTTGTGGTAAACCGCATCAAGGTAAGCCAGGAGCAGGGTGGGGCGCT
CAAGGACTGCGCCATTTTGTATCGCAGCAACGCCCAGTCGCGCGTGCTGGAAGAGGCGCTGTTGCAAACGGGGTTGCCTT
ACCGGATTTATGGAGGTATGCGCTTCTTCGAGCGTCAGGAAATCAAGGATGCGCTGGCCTATTTGCGGCTTATCGCCAAC
CGCAACGATGACGCCGCCTATGAACGGGTCGTCAATACGCCGCCGCGCGGCCTCGGCGATCGCACCCTGGATGTCGTGCG
TCAGACCGCCCGCGACCGTCAGCTGACGCTGTGGCAGTCGAGCCGGGCGCTGCTCGCGGAGCGCGTGCTGGCCGGTCGCG
CCGCCGCCGCGCTGCAGCGGTTTCTGGAGCTGATTGATGCGCTGGCGGAAGAGACGGCGGAGCTGCCGTTGCATGTGCAA
ACCGATCGGGTGATCAAAAACTCCGGCCTATGGAGCATGTACGAGCAGGAAAAAGGTGAAAAGGGCCAGGCGCGTATCGA
GAACCTGGAAGAGCTGGTCACCGCTACCCGCCAGTTTAGCTACAACGATGAAGATCAGGATTTGCTGCCGCTGCAGGCGT
TCCTGTCTCACGCCGCCCTGGAGGCCGGCGAGGGCCAGGCCGACTCCTATCAGGACGCGGTGCAATTAATGACGCTGCAT
TCCGCCAAAGGGCTAGAATTCCCCCAGGTGTTCATTGTCGGTATGGAAGAGGGAATGTTCCCCAGCCAGATGTCGCTGGA
CGAAGGCGGGCGCCTAGAAGAAGAGCGCCGCCTGGCGTATGTCGGCGTCACCCGCGCGATGGACAAGCTGACCATTACCT
ATGCGGAAACCCGCCGGCTGTATGGCAAAGAGGCTTACCACCGGCCGTCGCGCTTTGTCGGCGAACTGCCGCCGGCGTGC
GTGGAAGAAGTGCGGCTGCGCGCCAGCGTGACCCGTCCGGCCAGCCAGCAGCGCATTGGTGCGCCGTTAAGCGAAAGCGA
TAGCGGTTTCACCCTTGGCCAGCGGGTTCGCCATCCGAAATTTGGTGAGGGTACGGTAGTCAATCTGGAAGGCAGCGGCG
AACATAGCCGTCTGCAAATTGCGTTTCAAGGCCAGGGCATCAAATGGCTGGTGGTTGCTTACGCCCGCCTGGAAACCGTT
TGA

Upstream 100 bases:

>100_bases
ATCCCTGACAGCCTTGTTATAATCAACGCTAACTCTGTATAAATGACCAGTGTTTCGCCGCCGCCGGGCGGCGTTCCTCA
TCGCATGTTAAAGGCCACCT

Downstream 100 bases:

>100_bases
CCCGCCGCGCTATTGTACAGAAACTGGCCAAACGGTTTTTCTCACGTATTGACAGCCTTTTTCTTCTCGGCGTAACATGC
GCGCACTACTATTAGGAGAG

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MDVSDLLNSLNDKQREAVVAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENCSPYSVMAVTFTNKAAAEMRHRIEHLVGT
SQGGMWIGTFHGLAHRLLRAHHQDANLPQDFQILDSEDQLRLLKRLIRAMNLDEKQWPPRQAMWYIGGKKDDGLRPQHVE
SYGNPVEATWQRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILRHYRERFTNILVDEFQDTNQIQYAWIRMLAGDD
GNVMIVGDDDQSIYGWRGAQVENIQRFLDDFPGAQTIRLEQNYRSTSNILQAANALIAHNGGRLGKNLWTEGAEGEPITL
YCAFNELDEALFVVNRIKVSQEQGGALKDCAILYRSNAQSRVLEEALLQTGLPYRIYGGMRFFERQEIKDALAYLRLIAN
RNDDAAYERVVNTPPRGLGDRTLDVVRQTARDRQLTLWQSSRALLAERVLAGRAAAALQRFLELIDALAEETAELPLHVQ
TDRVIKNSGLWSMYEQEKGEKGQARIENLEELVTATRQFSYNDEDQDLLPLQAFLSHAALEAGEGQADSYQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMDKLTITYAETRRLYGKEAYHRPSRFVGELPPAC
VEEVRLRASVTRPASQQRIGAPLSESDSGFTLGQRVRHPKFGEGTVVNLEGSGEHSRLQIAFQGQGIKWLVVAYARLETV

Sequences:

>Translated_720_residues
MDVSDLLNSLNDKQREAVVAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENCSPYSVMAVTFTNKAAAEMRHRIEHLVGT
SQGGMWIGTFHGLAHRLLRAHHQDANLPQDFQILDSEDQLRLLKRLIRAMNLDEKQWPPRQAMWYIGGKKDDGLRPQHVE
SYGNPVEATWQRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILRHYRERFTNILVDEFQDTNQIQYAWIRMLAGDD
GNVMIVGDDDQSIYGWRGAQVENIQRFLDDFPGAQTIRLEQNYRSTSNILQAANALIAHNGGRLGKNLWTEGAEGEPITL
YCAFNELDEALFVVNRIKVSQEQGGALKDCAILYRSNAQSRVLEEALLQTGLPYRIYGGMRFFERQEIKDALAYLRLIAN
RNDDAAYERVVNTPPRGLGDRTLDVVRQTARDRQLTLWQSSRALLAERVLAGRAAAALQRFLELIDALAEETAELPLHVQ
TDRVIKNSGLWSMYEQEKGEKGQARIENLEELVTATRQFSYNDEDQDLLPLQAFLSHAALEAGEGQADSYQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMDKLTITYAETRRLYGKEAYHRPSRFVGELPPAC
VEEVRLRASVTRPASQQRIGAPLSESDSGFTLGQRVRHPKFGEGTVVNLEGSGEHSRLQIAFQGQGIKWLVVAYARLETV
>Mature_720_residues
MDVSDLLNSLNDKQREAVVAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENCSPYSVMAVTFTNKAAAEMRHRIEHLVGT
SQGGMWIGTFHGLAHRLLRAHHQDANLPQDFQILDSEDQLRLLKRLIRAMNLDEKQWPPRQAMWYIGGKKDDGLRPQHVE
SYGNPVEATWQRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILRHYRERFTNILVDEFQDTNQIQYAWIRMLAGDD
GNVMIVGDDDQSIYGWRGAQVENIQRFLDDFPGAQTIRLEQNYRSTSNILQAANALIAHNGGRLGKNLWTEGAEGEPITL
YCAFNELDEALFVVNRIKVSQEQGGALKDCAILYRSNAQSRVLEEALLQTGLPYRIYGGMRFFERQEIKDALAYLRLIAN
RNDDAAYERVVNTPPRGLGDRTLDVVRQTARDRQLTLWQSSRALLAERVLAGRAAAALQRFLELIDALAEETAELPLHVQ
TDRVIKNSGLWSMYEQEKGEKGQARIENLEELVTATRQFSYNDEDQDLLPLQAFLSHAALEAGEGQADSYQDAVQLMTLH
SAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMDKLTITYAETRRLYGKEAYHRPSRFVGELPPAC
VEEVRLRASVTRPASQQRIGAPLSESDSGFTLGQRVRHPKFGEGTVVNLEGSGEHSRLQIAFQGQGIKWLVVAYARLETV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=720, Percent_Identity=86.8055555555556, Blast_Score=1324, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=640, Percent_Identity=38.125, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=341, Percent_Identity=28.4457478005865, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=800, Percent_Identity=27.625, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81322; Mature: 81322

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSDLLNSLNDKQREAVVAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENCSPYSVMAV
CCHHHHHHHCCCHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
TFTNKAAAEMRHRIEHLVGTSQGGMWIGTFHGLAHRLLRAHHQDANLPQDFQILDSEDQL
EECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHH
RLLKRLIRAMNLDEKQWPPRQAMWYIGGKKDDGLRPQHVESYGNPVEATWQRIYQAYQEA
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAELLLRAHELWLNKPHILRHYRERFTNILVDEFQDTNQIQYAWIRMLAGDD
HHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHEEEECCC
GNVMIVGDDDQSIYGWRGAQVENIQRFLDDFPGAQTIRLEQNYRSTSNILQAANALIAHN
CCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GGRLGKNLWTEGAEGEPITLYCAFNELDEALFVVNRIKVSQEQGGALKDCAILYRSNAQS
CCCCCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCHH
RVLEEALLQTGLPYRIYGGMRFFERQEIKDALAYLRLIANRNDDAAYERVVNTPPRGLGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
RTLDVVRQTARDRQLTLWQSSRALLAERVLAGRAAAALQRFLELIDALAEETAELPLHVQ
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TDRVIKNSGLWSMYEQEKGEKGQARIENLEELVTATRQFSYNDEDQDLLPLQAFLSHAAL
CCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAGEGQADSYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAY
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
VGVTRAMDKLTITYAETRRLYGKEAYHRPSRFVGELPPACVEEVRLRASVTRPASQQRIG
HHHHHHHHHHEEEHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCC
APLSESDSGFTLGQRVRHPKFGEGTVVNLEGSGEHSRLQIAFQGQGIKWLVVAYARLETV
CCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCC
>Mature Secondary Structure
MDVSDLLNSLNDKQREAVVAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENCSPYSVMAV
CCHHHHHHHCCCHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
TFTNKAAAEMRHRIEHLVGTSQGGMWIGTFHGLAHRLLRAHHQDANLPQDFQILDSEDQL
EECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHH
RLLKRLIRAMNLDEKQWPPRQAMWYIGGKKDDGLRPQHVESYGNPVEATWQRIYQAYQEA
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAELLLRAHELWLNKPHILRHYRERFTNILVDEFQDTNQIQYAWIRMLAGDD
HHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHEEEECCC
GNVMIVGDDDQSIYGWRGAQVENIQRFLDDFPGAQTIRLEQNYRSTSNILQAANALIAHN
CCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GGRLGKNLWTEGAEGEPITLYCAFNELDEALFVVNRIKVSQEQGGALKDCAILYRSNAQS
CCCCCHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHCCCHH
RVLEEALLQTGLPYRIYGGMRFFERQEIKDALAYLRLIANRNDDAAYERVVNTPPRGLGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCH
RTLDVVRQTARDRQLTLWQSSRALLAERVLAGRAAAALQRFLELIDALAEETAELPLHVQ
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TDRVIKNSGLWSMYEQEKGEKGQARIENLEELVTATRQFSYNDEDQDLLPLQAFLSHAAL
CCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAGEGQADSYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAY
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
VGVTRAMDKLTITYAETRRLYGKEAYHRPSRFVGELPPACVEEVRLRASVTRPASQQRIG
HHHHHHHHHHEEEHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCC
APLSESDSGFTLGQRVRHPKFGEGTVVNLEGSGEHSRLQIAFQGQGIKWLVVAYARLETV
CCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]