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The map label for this gene is ilvR [H]

Identifier: 85059964

GI number: 85059964

Start: 3431686

End: 3432561

Strand: Reverse

Name: ilvR [H]

Synonym: SG1986

Alternate gene names: 85059964

Gene position: 3432561-3431686 (Counterclockwise)

Preceding gene: 85059966

Following gene: 85059958

Centisome position: 82.29

GC content: 54.91

Gene sequence:

>876_bases
ATGGACATTCGGCAACTCCGGGCGTTTGTGACTCTGGCTGAGTGCCTGAGCTATCGGGAAGCAGCGGAAAGATTGTGGCT
GACACAGCCCACGCTGACCAAACAGATCAAGATGCTGGAGACGGAAATCAGCCTGGTGCTGTTTTTGCGCGATAATCACG
GGACTCAGCTCAGCCGTCATGGTCAGCAGCTTTATCAGGATGCGAAGAAATTACTGAGTCAGTTCAACGCCTTCAAGACG
CTGTGCCAGCAATTGACCAACGGCAAGCGCGGCGCATTGTCGCTGGGGTTTATTTCCTCGGCGACGCCGGTAATGCCGGC
CATTATCACGGCCTTTCAGAATGCCTTTCCGGATATCCATCTTGGGCTACAGGATATCTCGTCGCCGGTGCAGCAAGAAC
GTATCCTGAACGGTCAGCTACAGGCAGGTTTTATGCGTATCCCGGTCTCGCCGCCGCTGGCGTTTCGTAAAACCGGCTCG
GACTGTCTAACGCTAGTTTTTCATAAAAGCTGCCGGTACCAGCAGGAACCGCTGCATCAGCTGTGGCGCCGCTGTACGCT
GTTTATCGTGAATGCGGAAACCTGCCCTGGTTCGGCGCTACAAATCAATAATTTTATTGCCGCGCACGGCATAGACGCGC
GCAAGATGCAATGTATCAGCGACACCCGTACCTTGATGACCATGGTGGAATCCCGTATGGGGGTAGCTATTTTACCGCAA
AGCGCCGTTATGGTGCTCCATCAGGATATTATCTGCCAGCCGCTGCACGGTGAATTCGCCCGCTGGGATATCGGCCTGGT
GTGGAACGAAAAGATTGCCGACCCGGTGCGTGACAGTTTTATAAGCCAGGTTACCGCGATGCTGGCGCCCGCCTGA

Upstream 100 bases:

>100_bases
CATAAAGAAAGACCCCTGCAGATTAATTATTCATGACAGTTCAACATGAAAGGGGTAATTTGTATAATTGAAAAAAGTTA
ATTGGCGATAGGAGTTTGGA

Downstream 100 bases:

>100_bases
CGGCGTCCGGCGCCGCTCGCGCGACGGCAAGGACATCATGCCTCAGGAAGGGGTAGCTGGTGCAGTTAAAGCACCACGCA
CGTTAAGCGGCAAAATGGGA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Isoleucine biosynthesis transcriptional activator [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDIRQLRAFVTLAECLSYREAAERLWLTQPTLTKQIKMLETEISLVLFLRDNHGTQLSRHGQQLYQDAKKLLSQFNAFKT
LCQQLTNGKRGALSLGFISSATPVMPAIITAFQNAFPDIHLGLQDISSPVQQERILNGQLQAGFMRIPVSPPLAFRKTGS
DCLTLVFHKSCRYQQEPLHQLWRRCTLFIVNAETCPGSALQINNFIAAHGIDARKMQCISDTRTLMTMVESRMGVAILPQ
SAVMVLHQDIICQPLHGEFARWDIGLVWNEKIADPVRDSFISQVTAMLAPA

Sequences:

>Translated_291_residues
MDIRQLRAFVTLAECLSYREAAERLWLTQPTLTKQIKMLETEISLVLFLRDNHGTQLSRHGQQLYQDAKKLLSQFNAFKT
LCQQLTNGKRGALSLGFISSATPVMPAIITAFQNAFPDIHLGLQDISSPVQQERILNGQLQAGFMRIPVSPPLAFRKTGS
DCLTLVFHKSCRYQQEPLHQLWRRCTLFIVNAETCPGSALQINNFIAAHGIDARKMQCISDTRTLMTMVESRMGVAILPQ
SAVMVLHQDIICQPLHGEFARWDIGLVWNEKIADPVRDSFISQVTAMLAPA
>Mature_291_residues
MDIRQLRAFVTLAECLSYREAAERLWLTQPTLTKQIKMLETEISLVLFLRDNHGTQLSRHGQQLYQDAKKLLSQFNAFKT
LCQQLTNGKRGALSLGFISSATPVMPAIITAFQNAFPDIHLGLQDISSPVQQERILNGQLQAGFMRIPVSPPLAFRKTGS
DCLTLVFHKSCRYQQEPLHQLWRRCTLFIVNAETCPGSALQINNFIAAHGIDARKMQCISDTRTLMTMVESRMGVAILPQ
SAVMVLHQDIICQPLHGEFARWDIGLVWNEKIADPVRDSFISQVTAMLAPA

Specific function: Positively regulates the expression of the ilvD gene while negatively autoregulating its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=26.4406779661017, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787879, Length=275, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788297, Length=288, Percent_Identity=23.9583333333333, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI2367136, Length=301, Percent_Identity=21.9269102990033, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=292, Percent_Identity=24.3150684931507, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=23.5494880546075, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=151, Percent_Identity=28.476821192053, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=31.2056737588652, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787530, Length=134, Percent_Identity=30.5970149253731, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=28, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=27.5303643724696, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32720; Mature: 32720

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLRAFVTLAECLSYREAAERLWLTQPTLTKQIKMLETEISLVLFLRDNHGTQLSRH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
GQQLYQDAKKLLSQFNAFKTLCQQLTNGKRGALSLGFISSATPVMPAIITAFQNAFPDIH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE
LGLQDISSPVQQERILNGQLQAGFMRIPVSPPLAFRKTGSDCLTLVFHKSCRYQQEPLHQ
ECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHH
LWRRCTLFIVNAETCPGSALQINNFIAAHGIDARKMQCISDTRTLMTMVESRMGVAILPQ
HHHHHEEEEEECCCCCCCCEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCH
SAVMVLHQDIICQPLHGEFARWDIGLVWNEKIADPVRDSFISQVTAMLAPA
HHHHHHHHHHHCCCCCCCCHHEEEEEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIRQLRAFVTLAECLSYREAAERLWLTQPTLTKQIKMLETEISLVLFLRDNHGTQLSRH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
GQQLYQDAKKLLSQFNAFKTLCQQLTNGKRGALSLGFISSATPVMPAIITAFQNAFPDIH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCE
LGLQDISSPVQQERILNGQLQAGFMRIPVSPPLAFRKTGSDCLTLVFHKSCRYQQEPLHQ
ECHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHH
LWRRCTLFIVNAETCPGSALQINNFIAAHGIDARKMQCISDTRTLMTMVESRMGVAILPQ
HHHHHEEEEEECCCCCCCCEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCH
SAVMVLHQDIICQPLHGEFARWDIGLVWNEKIADPVRDSFISQVTAMLAPA
HHHHHHHHHHHCCCCCCCCHHEEEEEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8113165; 11259647 [H]