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The map label for this gene is lepB [H]

Identifier: 85059766

GI number: 85059766

Start: 3052495

End: 3053481

Strand: Reverse

Name: lepB [H]

Synonym: SG1788

Alternate gene names: 85059766

Gene position: 3053481-3052495 (Counterclockwise)

Preceding gene: 85059767

Following gene: 85059765

Centisome position: 73.2

GC content: 55.22

Gene sequence:

>987_bases
ATGGCCAATATGTTTGCCTTGATAATGGCAATTGCGACGCTTATCACCGGGATCCTCTGGTGTTTGGAGCGTTTTAAACT
TGCGCCTGCCCGCCGCCGTCTGGGGCAGCAGCCCGGCCAGCAACGACCAGAAGCTGTGGGGGGAACGCCCGGTAATGATG
TCGCGGTAAAGGTCAGCCATAAGCCGGGCTGGATTGAAACCTGCGCGTCGATTTTTCCGGTCTTGTTACTGGTCTTTGTC
GTGCGCTCATTTATTTTCGAGCCGTTTCAGATTCCCTCAGGATCGATGATGCCGACGCTTCTGGTCGGTGATTTCATTCT
GGTGGAAAAATTTGCCTACGGCATTAAAGATCCCATTACCCATACAACCCTGATTGAAACCGGCCATCCCAAACGCGGCG
ACGTGGTGGTGTTTAAATTCCCGCTTGACCCCAAGCTGGACTATATTAAACGTGTTGTAGGGTTGCCGGGCGACCGGGTC
AGTTATGATCCTGTCAACAAGCGCTTAACGGTGCAGCCTGGCTGCGCCAGCGGTCAGGATTGCGCCACGGCGGTGCCGAT
TACTTACAGCGAACGCGCGCCGAGCGATTTTGAGCAAACCTTTAATTCAACCGGTGACGGCGAAGCCAGCAGTGGCTTCC
TGCAGGTGCCGCCCGATCGGGAGGTGGACGGCGCCATACGCCTGGCGCAGCGTAAAGAATCGTTGGGCGGCGTAGTGCAC
AACATTCTGATGGTGCCGGGGAAACAGGATCAACTGGGGATTTATTACCAACAGCAGGGCAGTTTGCTGGCGGAGTGGGT
GGTGCCCCAGGGGGAATATTTCATGATGGGCGACAATCGGGATAATAGCGCCGATAGCCGTTTTTGGGGTTTCGTACCGG
AAAGAAATCTGGTGGGTAAAGCGACGGCTATCTGGATGAGTTTCGAGAAGCAGGAAGGCCAATGGCCGACAGGCGTGCGC
TTAAGCCGTATTGGCGGCATTCATTAA

Upstream 100 bases:

>100_bases
AAAACGCATGAAGCAGGTAGGTAACGTTGAGCTTCCGCAGGAAGCGTTCCTGGCAATTCTGCATGTGGGTAAAGACAGCA
AATAAGTTTGAGGAGATTGC

Downstream 100 bases:

>100_bases
TTCCTTTTTTAGCGAAGCATTATTTCGCTTACCGATGTAGAATACCTCTCAGGTAATAAAAGGCTGGCTCCCTTCGAGGG
AGCCACTGCAAACGAAACAG

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MANMFALIMAIATLITGILWCLERFKLAPARRRLGQQPGQQRPEAVGGTPGNDVAVKVSHKPGWIETCASIFPVLLLVFV
VRSFIFEPFQIPSGSMMPTLLVGDFILVEKFAYGIKDPITHTTLIETGHPKRGDVVVFKFPLDPKLDYIKRVVGLPGDRV
SYDPVNKRLTVQPGCASGQDCATAVPITYSERAPSDFEQTFNSTGDGEASSGFLQVPPDREVDGAIRLAQRKESLGGVVH
NILMVPGKQDQLGIYYQQQGSLLAEWVVPQGEYFMMGDNRDNSADSRFWGFVPERNLVGKATAIWMSFEKQEGQWPTGVR
LSRIGGIH

Sequences:

>Translated_328_residues
MANMFALIMAIATLITGILWCLERFKLAPARRRLGQQPGQQRPEAVGGTPGNDVAVKVSHKPGWIETCASIFPVLLLVFV
VRSFIFEPFQIPSGSMMPTLLVGDFILVEKFAYGIKDPITHTTLIETGHPKRGDVVVFKFPLDPKLDYIKRVVGLPGDRV
SYDPVNKRLTVQPGCASGQDCATAVPITYSERAPSDFEQTFNSTGDGEASSGFLQVPPDREVDGAIRLAQRKESLGGVVH
NILMVPGKQDQLGIYYQQQGSLLAEWVVPQGEYFMMGDNRDNSADSRFWGFVPERNLVGKATAIWMSFEKQEGQWPTGVR
LSRIGGIH
>Mature_327_residues
ANMFALIMAIATLITGILWCLERFKLAPARRRLGQQPGQQRPEAVGGTPGNDVAVKVSHKPGWIETCASIFPVLLLVFVV
RSFIFEPFQIPSGSMMPTLLVGDFILVEKFAYGIKDPITHTTLIETGHPKRGDVVVFKFPLDPKLDYIKRVVGLPGDRVS
YDPVNKRLTVQPGCASGQDCATAVPITYSERAPSDFEQTFNSTGDGEASSGFLQVPPDREVDGAIRLAQRKESLGGVVHN
ILMVPGKQDQLGIYYQQQGSLLAEWVVPQGEYFMMGDNRDNSADSRFWGFVPERNLVGKATAIWMSFEKQEGQWPTGVRL
SRIGGIH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=328, Percent_Identity=68.2926829268293, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 36181; Mature: 36050

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMFALIMAIATLITGILWCLERFKLAPARRRLGQQPGQQRPEAVGGTPGNDVAVKVSH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
KPGWIETCASIFPVLLLVFVVRSFIFEPFQIPSGSMMPTLLVGDFILVEKFAYGIKDPIT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
HTTLIETGHPKRGDVVVFKFPLDPKLDYIKRVVGLPGDRVSYDPVNKRLTVQPGCASGQD
CEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCC
CATAVPITYSERAPSDFEQTFNSTGDGEASSGFLQVPPDREVDGAIRLAQRKESLGGVVH
CCEECCEEECCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
NILMVPGKQDQLGIYYQQQGSLLAEWVVPQGEYFMMGDNRDNSADSRFWGFVPERNLVGK
HHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCCEECCCCCCCCCCC
ATAIWMSFEKQEGQWPTGVRLSRIGGIH
CEEEEEEEHHCCCCCCCCCCHHCCCCCC
>Mature Secondary Structure 
ANMFALIMAIATLITGILWCLERFKLAPARRRLGQQPGQQRPEAVGGTPGNDVAVKVSH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
KPGWIETCASIFPVLLLVFVVRSFIFEPFQIPSGSMMPTLLVGDFILVEKFAYGIKDPIT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
HTTLIETGHPKRGDVVVFKFPLDPKLDYIKRVVGLPGDRVSYDPVNKRLTVQPGCASGQD
CEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCC
CATAVPITYSERAPSDFEQTFNSTGDGEASSGFLQVPPDREVDGAIRLAQRKESLGGVVH
CCEECCEEECCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
NILMVPGKQDQLGIYYQQQGSLLAEWVVPQGEYFMMGDNRDNSADSRFWGFVPERNLVGK
HHHCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCCEECCCCCCCCCCC
ATAIWMSFEKQEGQWPTGVRLSRIGGIH
CEEEEEEEHHCCCCCCCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6311837; 9278503; 2202591; 1618816; 1546969; 8394311; 2551889; 9823901 [H]