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The map label for this gene is lepA [H]

Identifier: 85059767

GI number: 85059767

Start: 3053497

End: 3055296

Strand: Reverse

Name: lepA [H]

Synonym: SG1789

Alternate gene names: 85059767

Gene position: 3055296-3053497 (Counterclockwise)

Preceding gene: 85059768

Following gene: 85059766

Centisome position: 73.25

GC content: 55.44

Gene sequence:

>1800_bases
ATGAAGAATATACGCAACTTCTCCATTATTGCCCATATCGATCACGGCAAGTCGACGCTGTCCGACCGCCTGATACAGAC
CTGTGGTGGTCTGAGCGAACGTGAAATGGCGGCTCAGGTGCTGGACTCCATGGATCTGGAGCGTGAGCGCGGTATTACCA
TCAAAGCGCAAAGCGTGACGCTGGACTATAAAGCGCCCGATGGTCAGGTTTATCAGCTGAATTTCATTGATACCCCGGGG
CATGTGGACTTCTCCTATGAAGTTTCCCGATCGCTTGCGGCATGCGAAGGCGCGCTGCTGGTGGTGGACGCCGGACAAGG
GGTGGAGGCCCAGACGCTGGCCAACTGCTACACTGCGATGGAAATGGATTTGGAAGTGGTGCCGGTGCTGAATAAAATCG
ACCTGCCCGCCGCCGATCCCGATCGCGTAGCGCAGGAAATCGAAGATATCGTCGGCATTGACGCCACCGACGCGGTGCGT
TGTTCGGCCAAAACCGGTGTCGGCGTACCCGATGTGCTCGAACGGCTGGTGCGCGATATCCCGCCGCCGGAAGGCGATCC
AGCCGCACCTTTGCAGGCGCTGATTATTGACTCCTGGTTCGATAATTATTTGGGCGTGGTCTCGCTGGTGCGTATTAAGA
ACGGCACCTTGCGCAAAGGTGACAAAATCAAGGTAATGAGCACCGGTCAACTTTACAACGCGGACCGACTGGGTATTTTT
ACCCCGAAACGTATCGACCGTGAGGTGCTTAACTGCGGCGAAGTCGGCTGGCTGGTCTGTGCCATTAAAGATATTCACGG
CGCGCCGGTGGGGGATACTCTGACGCTGGCGCGTCAGCCGGCTGATAAAGTGCTGCCGGGCTTCAAGAAAGTGAAGCCGC
AGGTTTATGCCGGTCTGTTCCCGGTGAGCTCCGACGATTATGAGGCGTTTCGCGACGCCCTCGGCAAGCTCAGCCTCAAC
GATGCCTCGCTGTTTTACGAGCCTGAAAGTTCAACCGCGCTCGGCTTCGGCTTTCGCTGCGGCTTTCTTGGCCTGCTGCA
TATGGAAATCATCCAGGAGCGGCTAGAGCGTGAATACGATCTTGATCTGATCACCACCGCGCCGACGGTGATTTATGAGG
TGTTGACGACCGATAACACGACGGTATACGTAGACAGCCCCTCCAAGCTGCCGCCGCTGAATGGTATTAACGAATTGCGT
GAACCTGTCGCCGAATGTCATATGCTGCTGCCGCAGGCTTATCTGGGTAATGTCATCACGCTATGTATCGAGAAGCGTGG
CGTTCAGACCAACATGGTCTACCACGGCAGCCAGGTGGCGCTGACCTATGAGATCCCCATGGTCGAAGTGGTACTGGATT
TCTTTGACCGGCTGAAATCCACCTCGCGCGGTTATGCTTCGCTGGATTATGGGTTCAAGCGTTTTCAGAACTCGGACATG
GTGCGGGTCGATGTGCTGATTAACGGCGAACGGGTCGATGCGCTGGCGCTGATTACCCATCGGGATAATGCGCCGTACCG
TGGCCGCGAGTTTGTTGATAAGCTCCAGGAACTCATCCCGCGCCAGCAGTTTGATATCGCCATTCAGGCGGCCATCGGCA
ACCATATTATTGCCCGTTCCACTGTCAAGCAGCTGCGCAAAAACGTGCTGGCAAAATGCTACGGCGGCGACGTGAGCCGT
AAGAAAAAGCTGCTGCAAAAGCAGAAAGAAGGTAAAAAACGCATGAAGCAGGTAGGTAACGTTGAGCTTCCGCAGGAAGC
GTTCCTGGCAATTCTGCATGTGGGTAAAGACAGCAAATAA

Upstream 100 bases:

>100_bases
AAGATGCGTCCATTATTTTGTGGGCCGTAGGGGTCACGTTCCCCGCAGTGCGACCACCGGAACGACTTAGAGCGCCTGTT
TAAGAGAAAAACTTATTAAA

Downstream 100 bases:

>100_bases
GTTTGAGGAGATTGCATGGCCAATATGTTTGCCTTGATAATGGCAATTGCGACGCTTATCACCGGGATCCTCTGGTGTTT
GGAGCGTTTTAAACTTGCGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYKAPDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVPDVLERLVRDIPPPEGDPAAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQLYNADRLGIF
TPKRIDREVLNCGEVGWLVCAIKDIHGAPVGDTLTLARQPADKVLPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVIYEVLTTDNTTVYVDSPSKLPPLNGINELR
EPVAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGSQVALTYEIPMVEVVLDFFDRLKSTSRGYASLDYGFKRFQNSDM
VRVDVLINGERVDALALITHRDNAPYRGREFVDKLQELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDSK

Sequences:

>Translated_599_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYKAPDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVPDVLERLVRDIPPPEGDPAAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQLYNADRLGIF
TPKRIDREVLNCGEVGWLVCAIKDIHGAPVGDTLTLARQPADKVLPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVIYEVLTTDNTTVYVDSPSKLPPLNGINELR
EPVAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGSQVALTYEIPMVEVVLDFFDRLKSTSRGYASLDYGFKRFQNSDM
VRVDVLINGERVDALALITHRDNAPYRGREFVDKLQELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDSK
>Mature_599_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYKAPDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVPDVLERLVRDIPPPEGDPAAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQLYNADRLGIF
TPKRIDREVLNCGEVGWLVCAIKDIHGAPVGDTLTLARQPADKVLPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVIYEVLTTDNTTVYVDSPSKLPPLNGINELR
EPVAECHMLLPQAYLGNVITLCIEKRGVQTNMVYHGSQVALTYEIPMVEVVLDFFDRLKSTSRGYASLDYGFKRFQNSDM
VRVDVLINGERVDALALITHRDNAPYRGREFVDKLQELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDSK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=46.9217970049917, Blast_Score=585, Evalue=1e-167,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=46.2686567164179, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=42.2818791946309, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=41.4473684210526, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=44.5255474452555, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=35.625, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=44.1441441441441, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI53729339, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI53729337, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=33.5329341317365, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=33.5329341317365, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=91.1519198664441, Blast_Score=1096, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=28.3495145631068, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.8387096774194, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789559, Length=222, Percent_Identity=30.1801801801802, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790412, Length=330, Percent_Identity=24.8484848484848, Blast_Score=62, Evalue=9e-11,
Organism=Escherichia coli, GI1789737, Length=330, Percent_Identity=24.8484848484848, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.6199021207178, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=24.1900647948164, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=35.6164383561644, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.3057324840764, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=37.037037037037, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=37.037037037037, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=37.2413793103448, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=239, Percent_Identity=28.0334728033473, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=46.4226289517471, Blast_Score=528, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.75, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.75, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.2637362637363, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=36.5217391304348, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=40, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=243, Percent_Identity=27.9835390946502, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.5940099833611, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=45.3237410071942, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=36.241610738255, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=36.241610738255, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=36.241610738255, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=35.5555555555556, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28572034, Length=247, Percent_Identity=28.3400809716599, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921738, Length=343, Percent_Identity=25.9475218658892, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66353; Mature: 66353

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAPDGQVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVRCSAKTGVGVPDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPAAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQLYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCC
TPKRIDREVLNCGEVGWLVCAIKDIHGAPVGDTLTLARQPADKVLPGFKKVKPQVYAGLF
CCHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCHHCCCCCHHHHCCCHHHCCCHHHEEEC
PVSSDDYEAFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVIYEVLTTDNTTVYVDSPSKLPPLNGINELREPVAECHMLLPQAYLGNVIT
CEEEECCHHHHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGSQVALTYEIPMVEVVLDFFDRLKSTSRGYASLDYGFKRFQNSDM
HHHHHCCCEEEEEEECCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCE
VRVDVLINGERVDALALITHRDNAPYRGREFVDKLQELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAPDGQVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVRCSAKTGVGVPDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPAAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQLYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCC
TPKRIDREVLNCGEVGWLVCAIKDIHGAPVGDTLTLARQPADKVLPGFKKVKPQVYAGLF
CCHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCHHCCCCCHHHHCCCHHHCCCHHHEEEC
PVSSDDYEAFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVIYEVLTTDNTTVYVDSPSKLPPLNGINELREPVAECHMLLPQAYLGNVIT
CEEEECCHHHHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGSQVALTYEIPMVEVVLDFFDRLKSTSRGYASLDYGFKRFQNSDM
HHHHHCCCEEEEEEECCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCE
VRVDVLINGERVDALALITHRDNAPYRGREFVDKLQELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA