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The map label for this gene is iscS [H]

Identifier: 85059747

GI number: 85059747

Start: 3023969

End: 3025168

Strand: Reverse

Name: iscS [H]

Synonym: SG1769

Alternate gene names: 85059747

Gene position: 3025168-3023969 (Counterclockwise)

Preceding gene: 85059748

Following gene: 85059746

Centisome position: 72.53

GC content: 58.25

Gene sequence:

>1200_bases
ATGAAATTACCGATTTATCTTGATTACTCTGCCACTACGCCGGTCGATCCGCGGGTGGCGGGTAAGATGATGCAGCATCT
TACGCTGGGCGGTATTTTCGGTAATCCGGCTTCCCGCTCCCACCGTTATGGCTGGCAGGCAGAGGAAGCCGTTGATATTG
CCCGTAATCACATCGCCGCCCTGATGGGCGCCGATCCCCGCGAAATCGTTTTCACTTCTGGCGCCACCGAGTCCGATAAT
CTGGCCCTCAAAGGGGCGGCTCATGCCAGCCGTGCCAAAGGCCGCCACATTATTACCGCCATAACTGAACATAAAGCGGT
ATTGGACACTTGCCTGCAATTGGAGCAGGAAGGTTTCACTGTCACCAGACTGGTGCCGGCGGCGAACGGTATCGTCACTC
CGCAGCAGTTGGGCGATGCGCTACGTGATGATACGATTCTGGTGTCGTTAATGCATGTCAACAATGAAACCGGCGTGATT
CAGGACATCGCGGCATTCGGCGATCTGTGTCGTGCCCGCGGGGTGCTTTTCCACGTGGACGCCACCCAAAGCATAGGGAA
ATTGCCTATCGATCTTACCACGTTGCCGGTGGATCTGATGTCGTTCAGCGGCCACAAAGTGTACGGGCCGAAGGGCATCG
GTGGCTTGTTCGTGCGCCGCACGCCGCGGGTGCGGATCGAAGCCCAAATCCACGGCGGCGGCCACGAGCGGGGCATGCGC
TCCGGGACGCTGCCGGTACATCAAATTGTCGGGATGGGGGAGGCCTATCACCTCGCGGCGCAGGAGCTGGCGAGCGAAAT
GCCGCGCCTGAGGGCTTTGCGTGACCGCTTGTGGCAGGGACTGCAACAGATCGGCGGGGTGGCGGTGAATGGCTGTCTAG
ATCAGAGCGTGGCGACGGTGCTGAATGTCAGCTTTCGCGATGTTGATGGCGAATCGCTTATTATAGCCCTGAAAGATCTG
GCGGTATCAACGGGATCTGCCTGCACCTCCGCCAGCCTTAAGCCGTCTTATGTGCTGCGCGCGATGGGGTTGGATGAAGA
GCTTTCCCACAGCTCCATCCGCTTCTCTATAGGGCGCTTTACCACTCCTGAAGAAATAGAGTATGCCATCAGTCATACCC
GAACGGCCATTGCGCGTCTGCTTGAGGTTGCCCCTCAATGTGCCGCCGGCGCGGTGAACTGGTGCAATGCTAAGCCATAA

Upstream 100 bases:

>100_bases
CGCTTACCAACGGCCGGCCGCAGGAAACGATTAACGTCAACCTGCACGCTTGAGTACAATAAGGGCATCCGCTGTTATTG
ATGCCTGGAGTTAATGCTTC

Downstream 100 bases:

>100_bases
TGCCGACTTACGTAGGTAGCGTTACCGCTCTCCGACTGCGCCAGCGCGTGCGGATAATGACGCCAAGAGACGGCAGCGCT
TTTATTCCGCTTTGCCTCAA

Product: putative aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKLPIYLDYSATTPVDPRVAGKMMQHLTLGGIFGNPASRSHRYGWQAEEAVDIARNHIAALMGADPREIVFTSGATESDN
LALKGAAHASRAKGRHIITAITEHKAVLDTCLQLEQEGFTVTRLVPAANGIVTPQQLGDALRDDTILVSLMHVNNETGVI
QDIAAFGDLCRARGVLFHVDATQSIGKLPIDLTTLPVDLMSFSGHKVYGPKGIGGLFVRRTPRVRIEAQIHGGGHERGMR
SGTLPVHQIVGMGEAYHLAAQELASEMPRLRALRDRLWQGLQQIGGVAVNGCLDQSVATVLNVSFRDVDGESLIIALKDL
AVSTGSACTSASLKPSYVLRAMGLDEELSHSSIRFSIGRFTTPEEIEYAISHTRTAIARLLEVAPQCAAGAVNWCNAKP

Sequences:

>Translated_399_residues
MKLPIYLDYSATTPVDPRVAGKMMQHLTLGGIFGNPASRSHRYGWQAEEAVDIARNHIAALMGADPREIVFTSGATESDN
LALKGAAHASRAKGRHIITAITEHKAVLDTCLQLEQEGFTVTRLVPAANGIVTPQQLGDALRDDTILVSLMHVNNETGVI
QDIAAFGDLCRARGVLFHVDATQSIGKLPIDLTTLPVDLMSFSGHKVYGPKGIGGLFVRRTPRVRIEAQIHGGGHERGMR
SGTLPVHQIVGMGEAYHLAAQELASEMPRLRALRDRLWQGLQQIGGVAVNGCLDQSVATVLNVSFRDVDGESLIIALKDL
AVSTGSACTSASLKPSYVLRAMGLDEELSHSSIRFSIGRFTTPEEIEYAISHTRTAIARLLEVAPQCAAGAVNWCNAKP
>Mature_399_residues
MKLPIYLDYSATTPVDPRVAGKMMQHLTLGGIFGNPASRSHRYGWQAEEAVDIARNHIAALMGADPREIVFTSGATESDN
LALKGAAHASRAKGRHIITAITEHKAVLDTCLQLEQEGFTVTRLVPAANGIVTPQQLGDALRDDTILVSLMHVNNETGVI
QDIAAFGDLCRARGVLFHVDATQSIGKLPIDLTTLPVDLMSFSGHKVYGPKGIGGLFVRRTPRVRIEAQIHGGGHERGMR
SGTLPVHQIVGMGEAYHLAAQELASEMPRLRALRDRLWQGLQQIGGVAVNGCLDQSVATVLNVSFRDVDGESLIIALKDL
AVSTGSACTSASLKPSYVLRAMGLDEELSHSSIRFSIGRFTTPEEIEYAISHTRTAIARLLEVAPQCAAGAVNWCNAKP

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of ThiI in a transpersulfida

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=52.7415143603133, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI156713448, Length=421, Percent_Identity=31.8289786223278, Blast_Score=175, Evalue=8e-44,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=73.7662337662338, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789175, Length=218, Percent_Identity=30.7339449541284, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787970, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=52.34375, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=31.9875776397516, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=49.4764397905759, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=53.2637075718016, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42890; Mature: 42890

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATTPVDPRVAGKMMQHLTLGGIFGNPASRSHRYGWQAEEAVDIARNHIAA
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LMGADPREIVFTSGATESDNLALKGAAHASRAKGRHIITAITEHKAVLDTCLQLEQEGFT
HHCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCE
VTRLVPAANGIVTPQQLGDALRDDTILVSLMHVNNETGVIQDIAAFGDLCRARGVLFHVD
EEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEE
ATQSIGKLPIDLTTLPVDLMSFSGHKVYGPKGIGGLFVRRTPRVRIEAQIHGGGHERGMR
CCCCCCCCCEEEEECCHHHHHCCCCEEECCCCCCCEEEECCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYHLAAQELASEMPRLRALRDRLWQGLQQIGGVAVNGCLDQSVATV
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
LNVSFRDVDGESLIIALKDLAVSTGSACTSASLKPSYVLRAMGLDEELSHSSIRFSIGRF
HCCCEECCCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCEEEEECCC
TTPEEIEYAISHTRTAIARLLEVAPQCAAGAVNWCNAKP
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYSATTPVDPRVAGKMMQHLTLGGIFGNPASRSHRYGWQAEEAVDIARNHIAA
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LMGADPREIVFTSGATESDNLALKGAAHASRAKGRHIITAITEHKAVLDTCLQLEQEGFT
HHCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCE
VTRLVPAANGIVTPQQLGDALRDDTILVSLMHVNNETGVIQDIAAFGDLCRARGVLFHVD
EEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEE
ATQSIGKLPIDLTTLPVDLMSFSGHKVYGPKGIGGLFVRRTPRVRIEAQIHGGGHERGMR
CCCCCCCCCEEEEECCHHHHHCCCCEEECCCCCCCEEEECCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYHLAAQELASEMPRLRALRDRLWQGLQQIGGVAVNGCLDQSVATV
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
LNVSFRDVDGESLIIALKDLAVSTGSACTSASLKPSYVLRAMGLDEELSHSSIRFSIGRF
HCCCEECCCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHCCCCEEEEECCC
TTPEEIEYAISHTRTAIARLLEVAPQCAAGAVNWCNAKP
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA