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The map label for this gene is dam [C]

Identifier: 85059186

GI number: 85059186

Start: 2030297

End: 2030989

Strand: Reverse

Name: dam [C]

Synonym: SG1208

Alternate gene names: 85059186

Gene position: 2030989-2030297 (Counterclockwise)

Preceding gene: 85059187

Following gene: 85059185

Centisome position: 48.69

GC content: 55.7

Gene sequence:

>693_bases
ATGCAGATGAAACCTATCCTGAAATGGGCGGGCGGCAAGTCTCGCCTGATGCCCCGCTTGCGCCCCTTCCTGCCGGCGGC
TGACTGTCTGGTCGAACCTTTTGTCGGCGGTGCCTCGGTTTTCCTCGCTACTGACTACCCTCGCTATGTGCTGGCAGACA
TTAACCCGGACCTTATCAATTTCTACCAGGCGGTTGTTGATAGCCCGGAAAGCGTCATACAGGCAGCGCTGCCGCTGTTC
AGGCACGCTAACACTTCTGTTGACTATTTTCGTTACCGCACCAATTTTGCCCAATGGCGAGCGACCAGGGGCATTAAACG
TGCCGCCGTGTTCCTTTACCTCAATCGCCATGGCTACAACGGCCTGTGCCGCTACAACCTGCGTGGCGAATTTAACATCC
CGTATGAACATTACAAAAAGCCCTATTTCCCCGCCGCAGAAATCCGCCTGTTCGCCGAAAAGGCGCGCGATACCCATATC
CGGTTTGTCTGCCTGCCCTTTGAGGAAACACTGGCGCTCCATGTAGATGAGCACTACGCAGTGTATACCGACCCGTCTTA
TTTCCCGCCTGAAGACAGGGACGGCTTCACCGGTTATTACATCACGCCGTTGAGGAAATGCGCCTTACCGAGCTTCGCCG
CGAGGACACTCTCCTGGCGAAAAAGCAGGTTGACGACCAGCTGGAAACGGTGA

Upstream 100 bases:

>100_bases
CTGAAGCACCACTCCCTTGAGGATGCCTGCTATCAGGCGCAGGTGGTGGCAGAAATCTGGCAGCGTCATTCCGACGCTTT
TATCGAACATTGCTGAGGAC

Downstream 100 bases:

>100_bases
GTTCCAGTATTTACTGGCGCTTTGACCAGCGCGAAAAGCAAAAGGGCTTTGACAGCGCCCGCCGCTGGCTAACCGAGTCC
GTGGTTAAGGTGATGTTGCC

Product: putative DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MQMKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLF
RHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHI
RFVCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR

Sequences:

>Translated_230_residues
MQMKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLF
RHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHI
RFVCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
>Mature_230_residues
MQMKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLF
RHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHI
RFVCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=200, Percent_Identity=48, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 26793; Mature: 26793

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLIN
CCCCCCCEECCCHHHHCHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHH
FYQAVVDSPESVIQAALPLFRHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYN
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHCCHHEEEEEEEEECCCCC
GLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRFVCLPFEETLALHVDEHYA
CEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEE
VYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
EEECCCCCCCCCCCCCCEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQMKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLIN
CCCCCCCEECCCHHHHCHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHH
FYQAVVDSPESVIQAALPLFRHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYN
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHCCHHEEEEEEEEECCCCC
GLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRFVCLPFEETLALHVDEHYA
CEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEE
VYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
EEECCCCCCCCCCCCCCEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]