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The map label for this gene is sbcB

Identifier: 85059110

GI number: 85059110

Start: 1877272

End: 1878693

Strand: Reverse

Name: sbcB

Synonym: SG1132

Alternate gene names: 85059110

Gene position: 1878693-1877272 (Counterclockwise)

Preceding gene: 85059113

Following gene: 85059108

Centisome position: 45.04

GC content: 58.65

Gene sequence:

>1422_bases
ATGGCTAACCCGTCAACGCGCCCCACTTTTCTGGTGCACGATTACGAAACGTTCGGCAAGTATCCCGCTCTTGACCGGCC
AGCGCAGTTCGCGGGGATACGCGCCGATAGTCAATTTGAGACCCTGGGCGAGCCGGAGGTCTTTTTTTGCCGTCCGGCCG
ACGATTATTTACCGGACCCGGAGGCGGTAATGATTACCGGCATTACGCCTCAGCAGGCCCTGCGTGACGGTGTCACAGAA
GCGGAATTCGCCCGCCGTATACATCAATTATTCAGCGTGCCCGACACTTGCATATTAGGTTACAACAACGTCCGCTTCGA
CGATGAGGTCAGCCGCAACCTGTTTTATCGCAATTTTTACGATCCCTACGGCTGGAGCTGGCAGCAGGGTAATTCCTGTT
GGGACCTGCTCGACGTCGTGCGCGCCTGCTATGCCCTGCGCCCCAACGGCATCGAATGGCCGCTGAATGACGACGGGCTG
CCCAGCTTCCGGCTTGAGCATCTGACCCGGGCCAACGGGATTGAACATGCCAACGCGCATGATGCCATGGCCGACGTCTA
TGCCACTCTGGCGATGGCCAAACTGATGCGTCAGGCGCAGCCGAAGCTGTTCGATTATCTCTACCGCCACCGCGGCAAGC
GGCAGTTGAAATCGCTTGTCGACATCGCGACGATGAAACCGCTGGTGCATGTTTCGGGTATGTTTGGCGCCGCCCGGGGC
AACACCGCCTGGATTGCGCCGCTCGCCTGGCATCCCGACAATCCAAATGCGCTTATCGTGTGCGATCTCAGTGGCGATAT
GCAGGTGCTGGCGGATTTGGACAGCGATGCACTGCGTACCCGACTGTACACGCGACGCGACGCGCTGGGTGACAATACCG
CGGTTCCCTTGAAATTGGTGTACCTGAATAAGTGCCCGGTGCTGGCGCCCGCCAATACGCTACAGCCAATGGATGCCGAA
CGCCTCGGTATTCAGCGTCAACGCTGTCTGGATAATTTGACCTGGCTGCGCGCGCGGCCGGAACTGCGCGAGAAAGTGGT
GGCGCTATTTGCCGAAAGCGCGCCTTTTAAGTCTTCGGATGACGTGGATGCTCAGTTATATGATGGCTTTTTCAGCGATG
CCGACCGCGCCGCGATGGAGATCATTCTCGCCACCGCGCCGGAAAATCTGCCGGCGCTGGATATAAGCTTTGTCGACGCG
CGCCTGAAGCCGTTGCTGTTCCGCTATCGCGCGCGCAACTTCCCCCACACGCTGGACCACAATGAGCAGTTACGCTGGCT
TGAGCACCGCAAAACCCGCTTCACCCAGCCGCGGCTGGAGGCCTATGTGCAGCAGTTGGAGACGCTATATCTGGCCCATG
AAGGGGATGAGCACAAAACGCGGCTACTACGCGCGCTGTTCGAGTATTTGCAGACATTGTAA

Upstream 100 bases:

>100_bases
TCAACGTCAGCACCAGGCAATCGGCACCCCCTGAACATAATGGACGGCATAAAAATGACATATCAAGGAATTCAGTACCC
TACCAGGAAGGGGGCACAAC

Downstream 100 bases:

>100_bases
CGGGAAGGCTGCGGCGCCGCGCGGCGAAAACCACGTGTCATCAACAGCAGATACACCGCGGCGCCCCAACTGAGTCCGAG
CATCATGGAGCTGGCCTCCA

Product: exonuclease I

Products: NA

Alternate protein names: Exonuclease I; DNA deoxyribophosphodiesterase; dRPase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MANPSTRPTFLVHDYETFGKYPALDRPAQFAGIRADSQFETLGEPEVFFCRPADDYLPDPEAVMITGITPQQALRDGVTE
AEFARRIHQLFSVPDTCILGYNNVRFDDEVSRNLFYRNFYDPYGWSWQQGNSCWDLLDVVRACYALRPNGIEWPLNDDGL
PSFRLEHLTRANGIEHANAHDAMADVYATLAMAKLMRQAQPKLFDYLYRHRGKRQLKSLVDIATMKPLVHVSGMFGAARG
NTAWIAPLAWHPDNPNALIVCDLSGDMQVLADLDSDALRTRLYTRRDALGDNTAVPLKLVYLNKCPVLAPANTLQPMDAE
RLGIQRQRCLDNLTWLRARPELREKVVALFAESAPFKSSDDVDAQLYDGFFSDADRAAMEIILATAPENLPALDISFVDA
RLKPLLFRYRARNFPHTLDHNEQLRWLEHRKTRFTQPRLEAYVQQLETLYLAHEGDEHKTRLLRALFEYLQTL

Sequences:

>Translated_473_residues
MANPSTRPTFLVHDYETFGKYPALDRPAQFAGIRADSQFETLGEPEVFFCRPADDYLPDPEAVMITGITPQQALRDGVTE
AEFARRIHQLFSVPDTCILGYNNVRFDDEVSRNLFYRNFYDPYGWSWQQGNSCWDLLDVVRACYALRPNGIEWPLNDDGL
PSFRLEHLTRANGIEHANAHDAMADVYATLAMAKLMRQAQPKLFDYLYRHRGKRQLKSLVDIATMKPLVHVSGMFGAARG
NTAWIAPLAWHPDNPNALIVCDLSGDMQVLADLDSDALRTRLYTRRDALGDNTAVPLKLVYLNKCPVLAPANTLQPMDAE
RLGIQRQRCLDNLTWLRARPELREKVVALFAESAPFKSSDDVDAQLYDGFFSDADRAAMEIILATAPENLPALDISFVDA
RLKPLLFRYRARNFPHTLDHNEQLRWLEHRKTRFTQPRLEAYVQQLETLYLAHEGDEHKTRLLRALFEYLQTL
>Mature_472_residues
ANPSTRPTFLVHDYETFGKYPALDRPAQFAGIRADSQFETLGEPEVFFCRPADDYLPDPEAVMITGITPQQALRDGVTEA
EFARRIHQLFSVPDTCILGYNNVRFDDEVSRNLFYRNFYDPYGWSWQQGNSCWDLLDVVRACYALRPNGIEWPLNDDGLP
SFRLEHLTRANGIEHANAHDAMADVYATLAMAKLMRQAQPKLFDYLYRHRGKRQLKSLVDIATMKPLVHVSGMFGAARGN
TAWIAPLAWHPDNPNALIVCDLSGDMQVLADLDSDALRTRLYTRRDALGDNTAVPLKLVYLNKCPVLAPANTLQPMDAER
LGIQRQRCLDNLTWLRARPELREKVVALFAESAPFKSSDDVDAQLYDGFFSDADRAAMEIILATAPENLPALDISFVDAR
LKPLLFRYRARNFPHTLDHNEQLRWLEHRKTRFTQPRLEAYVQQLETLYLAHEGDEHKTRLLRALFEYLQTL

Specific function: Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase [H]

COG id: COG2925

COG function: function code L; Exonuclease I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788321, Length=473, Percent_Identity=68.4989429175476, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013620
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T; PF08411 Exonuc_X-T_C [H]

EC number: =3.1.11.1 [H]

Molecular weight: Translated: 54146; Mature: 54015

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPSTRPTFLVHDYETFGKYPALDRPAQFAGIRADSQFETLGEPEVFFCRPADDYLPDP
CCCCCCCCEEEEECHHHHCCCCCCCCCHHHCCCCCCCCHHHCCCCCEEEECCCCCCCCCC
EAVMITGITPQQALRDGVTEAEFARRIHQLFSVPDTCILGYNNVRFDDEVSRNLFYRNFY
CEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCC
DPYGWSWQQGNSCWDLLDVVRACYALRPNGIEWPLNDDGLPSFRLEHLTRANGIEHANAH
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DAMADVYATLAMAKLMRQAQPKLFDYLYRHRGKRQLKSLVDIATMKPLVHVSGMFGAARG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NTAWIAPLAWHPDNPNALIVCDLSGDMQVLADLDSDALRTRLYTRRDALGDNTAVPLKLV
CCEEEEEEEECCCCCCEEEEEECCCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
YLNKCPVLAPANTLQPMDAERLGIQRQRCLDNLTWLRARPELREKVVALFAESAPFKSSD
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
DVDAQLYDGFFSDADRAAMEIILATAPENLPALDISFVDARLKPLLFRYRARNFPHTLDH
CCCHHHHHHHHCCHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
NEQLRWLEHRKTRFTQPRLEAYVQQLETLYLAHEGDEHKTRLLRALFEYLQTL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANPSTRPTFLVHDYETFGKYPALDRPAQFAGIRADSQFETLGEPEVFFCRPADDYLPDP
CCCCCCCEEEEECHHHHCCCCCCCCCHHHCCCCCCCCHHHCCCCCEEEECCCCCCCCCC
EAVMITGITPQQALRDGVTEAEFARRIHQLFSVPDTCILGYNNVRFDDEVSRNLFYRNFY
CEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCC
DPYGWSWQQGNSCWDLLDVVRACYALRPNGIEWPLNDDGLPSFRLEHLTRANGIEHANAH
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DAMADVYATLAMAKLMRQAQPKLFDYLYRHRGKRQLKSLVDIATMKPLVHVSGMFGAARG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NTAWIAPLAWHPDNPNALIVCDLSGDMQVLADLDSDALRTRLYTRRDALGDNTAVPLKLV
CCEEEEEEEECCCCCCEEEEEECCCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
YLNKCPVLAPANTLQPMDAERLGIQRQRCLDNLTWLRARPELREKVVALFAESAPFKSSD
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
DVDAQLYDGFFSDADRAAMEIILATAPENLPALDISFVDARLKPLLFRYRARNFPHTLDH
CCCHHHHHHHHCCHHHHHHHEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCC
NEQLRWLEHRKTRFTQPRLEAYVQQLETLYLAHEGDEHKTRLLRALFEYLQTL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3539937; 9097040; 9278503; 1329027 [H]