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The map label for this gene is tbpA

Identifier: 85058411

GI number: 85058411

Start: 760407

End: 761381

Strand: Reverse

Name: tbpA

Synonym: SG0433

Alternate gene names: 85058411

Gene position: 761381-760407 (Counterclockwise)

Preceding gene: 85058412

Following gene: 85058410

Centisome position: 18.25

GC content: 58.46

Gene sequence:

>975_bases
GTGTTGAAAAAATGGTTATTCTGCCTGTTACTGGCCGCGCCGGCACTGGCCAAACCCATCCTGACGGTATACACCTATGA
CTCGTTCTCTTCGGAATGGGGTCCGGGGCCGGCGATAAAAACGGCGTTTGAAAAGGAGTGTGGTTGCGAACTGAAGTTTG
TCGCGCTGGAAGATGGAGTGGCGCTTTTGAACCGCTTACGTCTGGAAGGGAAAAACAGCCGGGCAGATATCGTGCTGGGT
CTGGACAACAACCTGATCCAGGCGGCGCGGGACACCGGACTGTTCGCCCCCCATGCCGTTGCGACCAAAGCGCTAACCCT
GCCCGGCAGCTGGCATAACGACCTCTTCCTGCCCTACAATTATGGCTATTTCGCGTTTGTCTATGACCAGACCAAACTCA
AGAACCCGCCCCGCAGCCTGCATGAGCTGGTGGACGACCCCGCGTCACTGAAGATCATTTACGAAGATCCGCGCACCAGC
ACCCCGGGGCTGGGACTGCTGCTCTGGGTGCAAAAGGTCTATGGCGACGCTGCGCCTGCGGCCTGGGAAAAGCTGGCCGC
GAAAACCGTGACCGTGACCAAAGGCTGGAGCGAAGCCTATGGTCTTTTCCTGAAAGGGGAATCGGACATGGTGCTAAGCT
ATACCACCTCGCCGGCCTACCATATTATCGAAGAAAAGAAAAATCAGTATGCGGCGGCGGCCTTTAGCGAAGGTCACTAT
CAGCAGGTGGAGGTGGCCGGACAACTGGCGGCCAGCAAGCAACAGGCTCTGGCCCATCGCTTTATGCAATTTATCCTTAC
CCCGGCTTTCCAGCAGGCCATTCCGACCGGCAACTGGATGTATCCGGTGATTGATACCGCGCTGCCCGCCGGTTTCACCC
CGCCACCGCAGCAGACATTAAGCTTTACCCCGCAGCAGGTGGCAGCCGGGCGCGCCGGGTGGGTCAGCGGCTGGCAGCAG
GCCGTTAGCCGTTAA

Upstream 100 bases:

>100_bases
GAAGACCCGTCGAACCTGATCCGGTTAATACCGGCGAAGGGATTTGAGAGTGCGCACTGCTGCCTCAAGTCCTTTGCTAC
CTTGTCCTTAGGAGCGCAAA

Downstream 100 bases:

>100_bases
TGTCTCCTGCAGGTCGTCACGGCGGCGCGTCCCCGCGGTCCCCGACGCCGACGCAACCGCCCCGGCACCGGGGCGGGCGC
AGCGGATCTGACCTGTTCCT

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLKKWLFCLLLAAPALAKPILTVYTYDSFSSEWGPGPAIKTAFEKECGCELKFVALEDGVALLNRLRLEGKNSRADIVLG
LDNNLIQAARDTGLFAPHAVATKALTLPGSWHNDLFLPYNYGYFAFVYDQTKLKNPPRSLHELVDDPASLKIIYEDPRTS
TPGLGLLLWVQKVYGDAAPAAWEKLAAKTVTVTKGWSEAYGLFLKGESDMVLSYTTSPAYHIIEEKKNQYAAAAFSEGHY
QQVEVAGQLAASKQQALAHRFMQFILTPAFQQAIPTGNWMYPVIDTALPAGFTPPPQQTLSFTPQQVAAGRAGWVSGWQQ
AVSR

Sequences:

>Translated_324_residues
MLKKWLFCLLLAAPALAKPILTVYTYDSFSSEWGPGPAIKTAFEKECGCELKFVALEDGVALLNRLRLEGKNSRADIVLG
LDNNLIQAARDTGLFAPHAVATKALTLPGSWHNDLFLPYNYGYFAFVYDQTKLKNPPRSLHELVDDPASLKIIYEDPRTS
TPGLGLLLWVQKVYGDAAPAAWEKLAAKTVTVTKGWSEAYGLFLKGESDMVLSYTTSPAYHIIEEKKNQYAAAAFSEGHY
QQVEVAGQLAASKQQALAHRFMQFILTPAFQQAIPTGNWMYPVIDTALPAGFTPPPQQTLSFTPQQVAAGRAGWVSGWQQ
AVSR
>Mature_324_residues
MLKKWLFCLLLAAPALAKPILTVYTYDSFSSEWGPGPAIKTAFEKECGCELKFVALEDGVALLNRLRLEGKNSRADIVLG
LDNNLIQAARDTGLFAPHAVATKALTLPGSWHNDLFLPYNYGYFAFVYDQTKLKNPPRSLHELVDDPASLKIIYEDPRTS
TPGLGLLLWVQKVYGDAAPAAWEKLAAKTVTVTKGWSEAYGLFLKGESDMVLSYTTSPAYHIIEEKKNQYAAAAFSEGHY
QQVEVAGQLAASKQQALAHRFMQFILTPAFQQAIPTGNWMYPVIDTALPAGFTPPPQQTLSFTPQQVAAGRAGWVSGWQQ
AVSR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=314, Percent_Identity=71.0191082802548, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35659; Mature: 35659

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKWLFCLLLAAPALAKPILTVYTYDSFSSEWGPGPAIKTAFEKECGCELKFVALEDGV
CHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCH
ALLNRLRLEGKNSRADIVLGLDNNLIQAARDTGLFAPHAVATKALTLPGSWHNDLFLPYN
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHEEEECCCCCCCCEEEEEC
YGYFAFVYDQTKLKNPPRSLHELVDDPASLKIIYEDPRTSTPGLGLLLWVQKVYGDAAPA
CCEEEEEEECHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHH
AWEKLAAKTVTVTKGWSEAYGLFLKGESDMVLSYTTSPAYHIIEEKKNQYAAAAFSEGHY
HHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHHCCCCEEEHHCCCCH
QQVEVAGQLAASKQQALAHRFMQFILTPAFQQAIPTGNWMYPVIDTALPAGFTPPPQQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEHHHHHHHCCCCCCCCCHHHH
SFTPQQVAAGRAGWVSGWQQAVSR
CCCHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKKWLFCLLLAAPALAKPILTVYTYDSFSSEWGPGPAIKTAFEKECGCELKFVALEDGV
CHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCH
ALLNRLRLEGKNSRADIVLGLDNNLIQAARDTGLFAPHAVATKALTLPGSWHNDLFLPYN
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHEEEECCCCCCCCEEEEEC
YGYFAFVYDQTKLKNPPRSLHELVDDPASLKIIYEDPRTSTPGLGLLLWVQKVYGDAAPA
CCEEEEEEECHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHH
AWEKLAAKTVTVTKGWSEAYGLFLKGESDMVLSYTTSPAYHIIEEKKNQYAAAAFSEGHY
HHHHHHHHEEEEECCCHHHCEEEEECCCCEEEEEECCCHHHHHHHHCCCCEEEHHCCCCH
QQVEVAGQLAASKQQALAHRFMQFILTPAFQQAIPTGNWMYPVIDTALPAGFTPPPQQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEHHHHHHHCCCCCCCCCHHHH
SFTPQQVAAGRAGWVSGWQQAVSR
CCCHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]