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The map label for this gene is thiP

Identifier: 85058410

GI number: 85058410

Start: 758686

End: 760290

Strand: Reverse

Name: thiP

Synonym: SG0432

Alternate gene names: 85058410

Gene position: 760290-758686 (Counterclockwise)

Preceding gene: 85058411

Following gene: 85058409

Centisome position: 18.23

GC content: 62.18

Gene sequence:

>1605_bases
GTGGCGGGCCGCGTCAAAGCAATGCCCGGTTGGCTGTGGCCCGGCGCTACGGCGGCGGCGGTGATTGCCGTTATCGCACT
CGCCGCCTTCAGCGCCCTGTGGCGTCACGTCCCCTCCCATCCCTGGCGTCCACTGCTGGCGGATCGTTATCTTTGGCATG
TGGTGTCTTTTACCTTTACTCAGGCCCTGCTATCGGCGCTGTGCGCCACCCTGCCGGCCGTAGCGCTGGCACGGGCGCTT
TACCGCCGACGGTTTCCCGGCCGCGCCTTGCTGCTGCGGTTCTGCGGTATGACGCTCGTGCTGCCGGTGTTGGTGGGCGT
TTTCGGCATTATGAGCGTTTTTGGCCAGCAAGGCTGGCTGGCCCAGCTGTGCCGGGTGCTACAGGTGGAATACCGTTTTT
CCCCCTACGGCCTGGGTGGCATCCTGCTGGCGCATGTTTTCTTCAACCTGCCGCTGGCGACGCGATTACTGCTGCAGGCG
CTGGAAAGTATTCCGGTGGAGCAACGGCAACTGGCGGCTCAATTAGGTATGACCGCCGGACCGTTTTTCCGCCTGGTCGA
ATGGCCCTACTTACGCCGTCAACTGCTTCCTGCGGCCGCGTTGATTTTTATGCTCTGTTTCGCCAGTTTCGCCACCGTAC
TGGCGCTGGGCGGCGGTCCGCGCGCGACGACGATCGAACTGGCGATTTATCAGGCCCTCAGCTACGACTTCGACGTCGGC
CGCGCCGCTTTGCTAGCGCTGGTGCAAATGGTCTGCTGCCTCGGGCTGGTAATGCTGAGTCAACGGCTGGCCAGCGCGCT
GCCGGTAGGGCATTCCCATCATGCCCGCTGGCGCGATCGCCAGACGGCGACAGGGGGAAGAATAGGCGACGCGTTGATTA
TCCTGGCGGCGCTACTCTTGTTGCTGCCGCCGCTGCTGGCGGTCGTCATCGACGGGATCAACGGCGATCTGCCGACGGTG
TTGCGGCAAACGGCGCTATGGCGGGCGGTATTCACCTCTCTGGGGGTTGCGCTGTGCGCCGGTCTGTTGTGCCTGCTGCT
TACCGGTATGTTGCTCTGGAGCAGCCGGGAACTGCGCCTGCGTCAGCGATGGCTGGGCGCCCAACTGTTGGATCTGAGCG
GCATGGTGATCCTGGCGATGCCCGGTATCGTGCTGGCGACAGGTTTTTTCCTGCTGGCAACCGATAACGGCGGATTACCC
GCCTCGCCGGCGCCGGTAATTATCCTCGCCAATGCGCTAATGGCTATTCCCTATGCGCTAAAGGTATTGGAAAGCCCGAT
GCAGGACGTAGCCGAATGCTACCGGCAACTGTGCCTCACGCTCAACATCGGAGGCCTCAACCGGTTACGTTTGGTCGAAT
GGCGCGCACTGCGCCAGCCGGCGGGACGTGCGCTAGCCTTCGCCTGTATCTTGTCCTTGGGGGATTTTGGCGTGATAGCG
CTCTTCGGCAGCGAGGATTTCCGTACCTTGCCGCTTTATCTTTACCAGCAGATCGGCGCCTATCGCAGCCAGGATGGCGC
GGTGACCGCCCTGGTGTTGCTGCTGCTGTGTCTGCTGCTGTTTACGTTGTTTGAAAAAGGGTCGGGGACCAATGATAACG
CTTGA

Upstream 100 bases:

>100_bases
TCACGGCGGCGCGTCCCCGCGGTCCCCGACGCCGACGCAACCGCCCCGGCACCGGGGCGGGCGCAGCGGATCTGACCTGT
TCCTCGCCCGGTCTGCGGCA

Downstream 100 bases:

>100_bases
TAAAGTGACCTGGCTTTATCGCCATTTACCCTTGCGGTTTGACCTGCACGTCGACGCCGGCGAAAAGGTGGCGATACTTG
GCCCGAGCGGCGCCGGTAAA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MAGRVKAMPGWLWPGATAAAVIAVIALAAFSALWRHVPSHPWRPLLADRYLWHVVSFTFTQALLSALCATLPAVALARAL
YRRRFPGRALLLRFCGMTLVLPVLVGVFGIMSVFGQQGWLAQLCRVLQVEYRFSPYGLGGILLAHVFFNLPLATRLLLQA
LESIPVEQRQLAAQLGMTAGPFFRLVEWPYLRRQLLPAAALIFMLCFASFATVLALGGGPRATTIELAIYQALSYDFDVG
RAALLALVQMVCCLGLVMLSQRLASALPVGHSHHARWRDRQTATGGRIGDALIILAALLLLLPPLLAVVIDGINGDLPTV
LRQTALWRAVFTSLGVALCAGLLCLLLTGMLLWSSRELRLRQRWLGAQLLDLSGMVILAMPGIVLATGFFLLATDNGGLP
ASPAPVIILANALMAIPYALKVLESPMQDVAECYRQLCLTLNIGGLNRLRLVEWRALRQPAGRALAFACILSLGDFGVIA
LFGSEDFRTLPLYLYQQIGAYRSQDGAVTALVLLLLCLLLFTLFEKGSGTNDNA

Sequences:

>Translated_534_residues
MAGRVKAMPGWLWPGATAAAVIAVIALAAFSALWRHVPSHPWRPLLADRYLWHVVSFTFTQALLSALCATLPAVALARAL
YRRRFPGRALLLRFCGMTLVLPVLVGVFGIMSVFGQQGWLAQLCRVLQVEYRFSPYGLGGILLAHVFFNLPLATRLLLQA
LESIPVEQRQLAAQLGMTAGPFFRLVEWPYLRRQLLPAAALIFMLCFASFATVLALGGGPRATTIELAIYQALSYDFDVG
RAALLALVQMVCCLGLVMLSQRLASALPVGHSHHARWRDRQTATGGRIGDALIILAALLLLLPPLLAVVIDGINGDLPTV
LRQTALWRAVFTSLGVALCAGLLCLLLTGMLLWSSRELRLRQRWLGAQLLDLSGMVILAMPGIVLATGFFLLATDNGGLP
ASPAPVIILANALMAIPYALKVLESPMQDVAECYRQLCLTLNIGGLNRLRLVEWRALRQPAGRALAFACILSLGDFGVIA
LFGSEDFRTLPLYLYQQIGAYRSQDGAVTALVLLLLCLLLFTLFEKGSGTNDNA
>Mature_533_residues
AGRVKAMPGWLWPGATAAAVIAVIALAAFSALWRHVPSHPWRPLLADRYLWHVVSFTFTQALLSALCATLPAVALARALY
RRRFPGRALLLRFCGMTLVLPVLVGVFGIMSVFGQQGWLAQLCRVLQVEYRFSPYGLGGILLAHVFFNLPLATRLLLQAL
ESIPVEQRQLAAQLGMTAGPFFRLVEWPYLRRQLLPAAALIFMLCFASFATVLALGGGPRATTIELAIYQALSYDFDVGR
AALLALVQMVCCLGLVMLSQRLASALPVGHSHHARWRDRQTATGGRIGDALIILAALLLLLPPLLAVVIDGINGDLPTVL
RQTALWRAVFTSLGVALCAGLLCLLLTGMLLWSSRELRLRQRWLGAQLLDLSGMVILAMPGIVLATGFFLLATDNGGLPA
SPAPVIILANALMAIPYALKVLESPMQDVAECYRQLCLTLNIGGLNRLRLVEWRALRQPAGRALAFACILSLGDFGVIAL
FGSEDFRTLPLYLYQQIGAYRSQDGAVTALVLLLLCLLLFTLFEKGSGTNDNA

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=528, Percent_Identity=66.6666666666667, Blast_Score=626, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57988; Mature: 57857

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRVKAMPGWLWPGATAAAVIAVIALAAFSALWRHVPSHPWRPLLADRYLWHVVSFTFT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QALLSALCATLPAVALARALYRRRFPGRALLLRFCGMTLVLPVLVGVFGIMSVFGQQGWL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AQLCRVLQVEYRFSPYGLGGILLAHVFFNLPLATRLLLQALESIPVEQRQLAAQLGMTAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PFFRLVEWPYLRRQLLPAAALIFMLCFASFATVLALGGGPRATTIELAIYQALSYDFDVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHH
RAALLALVQMVCCLGLVMLSQRLASALPVGHSHHARWRDRQTATGGRIGDALIILAALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHH
LLPPLLAVVIDGINGDLPTVLRQTALWRAVFTSLGVALCAGLLCLLLTGMLLWSSRELRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RQRWLGAQLLDLSGMVILAMPGIVLATGFFLLATDNGGLPASPAPVIILANALMAIPYAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KVLESPMQDVAECYRQLCLTLNIGGLNRLRLVEWRALRQPAGRALAFACILSLGDFGVIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEE
LFGSEDFRTLPLYLYQQIGAYRSQDGAVTALVLLLLCLLLFTLFEKGSGTNDNA
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AGRVKAMPGWLWPGATAAAVIAVIALAAFSALWRHVPSHPWRPLLADRYLWHVVSFTFT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QALLSALCATLPAVALARALYRRRFPGRALLLRFCGMTLVLPVLVGVFGIMSVFGQQGWL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AQLCRVLQVEYRFSPYGLGGILLAHVFFNLPLATRLLLQALESIPVEQRQLAAQLGMTAG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PFFRLVEWPYLRRQLLPAAALIFMLCFASFATVLALGGGPRATTIELAIYQALSYDFDVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHH
RAALLALVQMVCCLGLVMLSQRLASALPVGHSHHARWRDRQTATGGRIGDALIILAALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHH
LLPPLLAVVIDGINGDLPTVLRQTALWRAVFTSLGVALCAGLLCLLLTGMLLWSSRELRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RQRWLGAQLLDLSGMVILAMPGIVLATGFFLLATDNGGLPASPAPVIILANALMAIPYAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
KVLESPMQDVAECYRQLCLTLNIGGLNRLRLVEWRALRQPAGRALAFACILSLGDFGVIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEE
LFGSEDFRTLPLYLYQQIGAYRSQDGAVTALVLLLLCLLLFTLFEKGSGTNDNA
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]