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The map label for this gene is leuD

Identifier: 85058412

GI number: 85058412

Start: 763922

End: 764524

Strand: Reverse

Name: leuD

Synonym: SG0434

Alternate gene names: 85058412

Gene position: 764524-763922 (Counterclockwise)

Preceding gene: 85058413

Following gene: 85058411

Centisome position: 18.33

GC content: 55.22

Gene sequence:

>603_bases
ATGGCTAAATTTACCCAACATACCGGCATCGTGGTGCCCCTTGACGCCGCCAACGTCGACACCGATGCCATCATCCCCAA
GCAGTTTCTGCAGAAGGTGACCCGCACCGGATTTGGCCAGCATTTATTCAATGACTGGCGCTTTCTGGACAACGCCGGGC
AACAACCTAACCCGGATTTCGTGCTGAATCAGCCGCGCTATCGCGGCGCCAGTATCCTGCTGGCGCGGGAAAACTTCGGC
TGTGGCTCCTCGCGCGAACATGCGCCCTGGGCATTGACCGATTACGGCTTTCATGCCGTGATAGCGCCGAGCTTTGCCGA
TATTTTCTACGGCAACAGCTTCAATAATCAATTGCTGCCGATTACGCTGTCGGAAGAGCAGATCAACGAACTGTTTGCTA
TGGTCGCGGCGCAGGAGGGGATGACATTTACCGTCGATCTGGAACGACAGCAGGTCGTGGCCGGCGATAAGGTTTATCCT
TTCGAGATAGACAGCTTCCGCCGCCATTACATGATAAACGGCCTCGACAGTATCGGTCTGACGCTGATGCACGATGAAGC
GATAAGCCAATACGAGAGCCGGCAGCCGGCATTTCTGAACTGA

Upstream 100 bases:

>100_bases
CGCGGCGGACGCACCCATCTGGTCAGCCCCGCGATGGCGGCGGCGGCGGCGGTAGCCGGCCGTTTCGCCGATATCCGCGA
ATTAAATTAAGGAAAAAGAG

Downstream 100 bases:

>100_bases
TCGTCGCCGTCGCGCCCGGCGGGTGCGACGGTCGGGCCTCGCCGTTCCTCTTTCACCACGGCGGTATCGAATACTCACTG
TGCAGACGGAACCCACCGCT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAKFTQHTGIVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDNAGQQPNPDFVLNQPRYRGASILLARENFG
CGSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLPITLSEEQINELFAMVAAQEGMTFTVDLERQQVVAGDKVYP
FEIDSFRRHYMINGLDSIGLTLMHDEAISQYESRQPAFLN

Sequences:

>Translated_200_residues
MAKFTQHTGIVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDNAGQQPNPDFVLNQPRYRGASILLARENFG
CGSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLPITLSEEQINELFAMVAAQEGMTFTVDLERQQVVAGDKVYP
FEIDSFRRHYMINGLDSIGLTLMHDEAISQYESRQPAFLN
>Mature_199_residues
AKFTQHTGIVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDNAGQQPNPDFVLNQPRYRGASILLARENFGC
GSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLPITLSEEQINELFAMVAAQEGMTFTVDLERQQVVAGDKVYPF
EIDSFRRHYMINGLDSIGLTLMHDEAISQYESRQPAFLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=78.7878787878788, Blast_Score=336, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=51.5151515151515, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SODGM (Q2NVW6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454114.1
- STRING:   Q2NVW6
- GeneID:   3867409
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0434
- NMPDR:   fig|343509.6.peg.1004
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q2NVW6
- ProtClustDB:   PRK01641
- BioCyc:   SGLO343509:SG0434-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22578; Mature: 22446

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFTQHTGIVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDNAGQQPNPDF
CCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCE
VLNQPRYRGASILLARENFGCGSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLP
EECCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCEEEE
ITLSEEQINELFAMVAAQEGMTFTVDLERQQVVAGDKVYPFEIDSFRRHYMINGLDSIGL
EEECHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCEECEEEHHHHHHHHHHCCCCCCCE
TLMHDEAISQYESRQPAFLN
EEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKFTQHTGIVVPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDNAGQQPNPDF
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCE
VLNQPRYRGASILLARENFGCGSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLP
EECCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCEEEE
ITLSEEQINELFAMVAAQEGMTFTVDLERQQVVAGDKVYPFEIDSFRRHYMINGLDSIGL
EEECHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCEECEEEHHHHHHHHHHCCCCCCCE
TLMHDEAISQYESRQPAFLN
EEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA