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The map label for this gene is ttcA [H]

Identifier: 84625816

GI number: 84625816

Start: 4696673

End: 4697590

Strand: Reverse

Name: ttcA [H]

Synonym: XOO_4159

Alternate gene names: 84625816

Gene position: 4697590-4696673 (Counterclockwise)

Preceding gene: 84625818

Following gene: 84625815

Centisome position: 95.09

GC content: 66.78

Gene sequence:

>918_bases
ATGACCGCTGTCCTGCCGCTGCCGCACCCCCTGGCCGACCCCGCGCCGCGCGACCCGCGCCAGCGGCTGCAGCGTGAACA
GCTGCGCCTGGGCAAACGCCTGCAGCGCCAGGTGGGTCAGGCGATTGCCGACTTCGGCATGATCTCACCCGGCGACAAGA
TCATGGTCTGCCTGTCTGGTGGCAAGGACAGCTACACCATGCTGGACATGCTGCTGCAGTTGCAGCGCAAGGCGCCGGTG
CCGTTCACGCTGGTGGCGGTGAACCTGGACCAGAAGCAGCCCGACTTCCCTGCGCATGTGCTGCCGGCCTACCTGGATGC
GCTGGGTGTGCCGTTCGACATCGTCGAACAGGACACCTATTCGGTGGTCAGCCGGGTGGTTCCGGCCGGCAAGACGATGT
GTTCGCTGTGCTCGCGGCTGCGGCGCGGTGCGTTATATGCCTACGCGCAGACGCACGGCGTGACCAAGATCGCACTCGGC
CATCACCGCGACGACATCGTGGCCACGTTCTTCATGAATCTGTTTCACCACGCGCGGCTGGCGGCGATGGCGCCCAAATT
GCGCAGCGACGACGGTGCGCATGTGGTGATTCGCCCGCTGGCCTACGTGCGCGAAGCTCACATCGCCGCGTACGCGCAGG
CGCGGCAGTTCCCGATCATTCCGTGCAACCTGTGCGGCAGCCAGGAAAACCTGCAGCGCCAGCAGGTCGGCAAGATGTTG
CAACACTGGGACCACGAGCAGCCCGGGCGCGTCGAGCAGATTGCCCGCGCCTTGGGCGATGTCCGCCCCGAACAACTGGC
CGACCGCACGTTGTTCGATTTCCTGGCGCTGGGCCGCAGCGGCGATGCGCCGCCCGATCTTGCGCCTGATCCCGGCGCGT
GGCTAACGGCCAGCGACGCCACCCACGATAGCGACTGA

Upstream 100 bases:

>100_bases
GTCAGCGTAAACGAACTCAGAAGGACAGCTGCCAGCCTGCCGCTCCGCACGCAGTCAACCCCGCCGCCTGCCAGCCCCCG
GCAGCTACAATGGGCGGCTT

Downstream 100 bases:

>100_bases
CGCGGCCGATCCGGCTGCCGGCAGTCTTCCACCCTCCACGTTTTTCTTCATTCGGTCCAACGCATGTTCTTTCGCAATCT
CACCCTGTTCCGCTTTCCCA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTAVLPLPHPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMISPGDKIMVCLSGGKDSYTMLDMLLQLQRKAPV
PFTLVAVNLDQKQPDFPAHVLPAYLDALGVPFDIVEQDTYSVVSRVVPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALG
HHRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREAHIAAYAQARQFPIIPCNLCGSQENLQRQQVGKML
QHWDHEQPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPPDLAPDPGAWLTASDATHDSD

Sequences:

>Translated_305_residues
MTAVLPLPHPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMISPGDKIMVCLSGGKDSYTMLDMLLQLQRKAPV
PFTLVAVNLDQKQPDFPAHVLPAYLDALGVPFDIVEQDTYSVVSRVVPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALG
HHRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREAHIAAYAQARQFPIIPCNLCGSQENLQRQQVGKML
QHWDHEQPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPPDLAPDPGAWLTASDATHDSD
>Mature_304_residues
TAVLPLPHPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMISPGDKIMVCLSGGKDSYTMLDMLLQLQRKAPVP
FTLVAVNLDQKQPDFPAHVLPAYLDALGVPFDIVEQDTYSVVSRVVPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGH
HRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREAHIAAYAQARQFPIIPCNLCGSQENLQRQQVGKMLQ
HWDHEQPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPPDLAPDPGAWLTASDATHDSD

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=298, Percent_Identity=55.0335570469799, Blast_Score=337, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI193211090, Length=260, Percent_Identity=31.5384615384615, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 33723; Mature: 33592

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVLPLPHPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMISPGDKIMVCLSG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GKDSYTMLDMLLQLQRKAPVPFTLVAVNLDQKQPDFPAHVLPAYLDALGVPFDIVEQDTY
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
SVVSRVVPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGHHRDDIVATFFMNLFHHARL
HHHHHHCCCCHHHHHHHHHHHHCCEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AAMAPKLRSDDGAHVVIRPLAYVREAHIAAYAQARQFPIIPCNLCGSQENLQRQQVGKML
HHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHH
QHWDHEQPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPPDLAPDPGAWLTASDA
HHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
THDSD
CCCCC
>Mature Secondary Structure 
TAVLPLPHPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMISPGDKIMVCLSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GKDSYTMLDMLLQLQRKAPVPFTLVAVNLDQKQPDFPAHVLPAYLDALGVPFDIVEQDTY
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
SVVSRVVPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGHHRDDIVATFFMNLFHHARL
HHHHHHCCCCHHHHHHHHHHHHCCEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AAMAPKLRSDDGAHVVIRPLAYVREAHIAAYAQARQFPIIPCNLCGSQENLQRQQVGKML
HHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHH
QHWDHEQPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPPDLAPDPGAWLTASDA
HHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
THDSD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]