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The map label for this gene is gph [C]

Identifier: 84624871

GI number: 84624871

Start: 3644881

End: 3645525

Strand: Reverse

Name: gph [C]

Synonym: XOO_3214

Alternate gene names: 84624871

Gene position: 3645525-3644881 (Counterclockwise)

Preceding gene: 84624872

Following gene: 84624870

Centisome position: 73.79

GC content: 65.12

Gene sequence:

>645_bases
GTGAGTGCAGCCACGCTGTTTTTCGATCTGGACGGCACGCTGGTCGATTCGGAGCCCGGCATCGTCGCCAGCATCGTGTA
TGCCTTCGACGAACTCGGGCAGCCGCGTCCATCTGCGCAGACCTTGCGCGCGTGGATCGGCCCGCCGTTGCGCGACAGCT
TCGCCGAGTGTTTTGCAAACGATCCAGGGCGGGGGCAGCGTGCGCTCGGGCTCTACCGCGCGCGCTACGACGCGGTGGGC
TGGACCGAGCTCAGCGTGTTCGACGGCATTGGCGAGGTGGTCGCCAGCCTGCAGCGCGCCGGTCATCGTCTGGCGGTGGT
GACCTCGAAGAATGAGCGCTATGCGCGGCGCATCGTCGAGCATCTGCCATTTGGCGCTTGCTTCGAGGAGGTGATCGGTG
CCAGCGAAGACGGCGAACGTCGCTTCAAGCCGGACCTCATTGCCGAAGCGCTGCGCCGTTTGCAGATCGAGAAAACTGGC
TGCGTGATGATCGGCGACCGGCGCATGGATATTGATGGCGCCAATCACCACGGCATCCATAGCATCGGCGTGTTATGGGG
CTTCGGCGACGAGGCTGAGTTGCGTGCCGCCGGTGCCGGTGCACTGGCACGCACCCCGGCTGACCTGGTGGCGTTGCTTC
AGTGA

Upstream 100 bases:

>100_bases
CCAAGGACGTCACCTACATTTCCACTGGCGGCGGTGCATTCCTGGAGTTTCTGGAAGGCAAGACCTTGCCGGCTGTGGCC
GCACTGCAGGCCCGCGGTCA

Downstream 100 bases:

>100_bases
TGAACAGTGCGCGTCGGCCGTTGCGTTGGCTTGGTCGACGCCTGCAGCCGCGGTTCGCATCGCGCTGAACACTCTTCATC
GCTGCCGGGTTAGGGTGGCG

Product: indigoidine synthesis-like protein

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSAATLFFDLDGTLVDSEPGIVASIVYAFDELGQPRPSAQTLRAWIGPPLRDSFAECFANDPGRGQRALGLYRARYDAVG
WTELSVFDGIGEVVASLQRAGHRLAVVTSKNERYARRIVEHLPFGACFEEVIGASEDGERRFKPDLIAEALRRLQIEKTG
CVMIGDRRMDIDGANHHGIHSIGVLWGFGDEAELRAAGAGALARTPADLVALLQ

Sequences:

>Translated_214_residues
MSAATLFFDLDGTLVDSEPGIVASIVYAFDELGQPRPSAQTLRAWIGPPLRDSFAECFANDPGRGQRALGLYRARYDAVG
WTELSVFDGIGEVVASLQRAGHRLAVVTSKNERYARRIVEHLPFGACFEEVIGASEDGERRFKPDLIAEALRRLQIEKTG
CVMIGDRRMDIDGANHHGIHSIGVLWGFGDEAELRAAGAGALARTPADLVALLQ
>Mature_213_residues
SAATLFFDLDGTLVDSEPGIVASIVYAFDELGQPRPSAQTLRAWIGPPLRDSFAECFANDPGRGQRALGLYRARYDAVGW
TELSVFDGIGEVVASLQRAGHRLAVVTSKNERYARRIVEHLPFGACFEEVIGASEDGERRFKPDLIAEALRRLQIEKTGC
VMIGDRRMDIDGANHHGIHSIGVLWGFGDEAELRAAGAGALARTPADLVALLQ

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=209, Percent_Identity=26.7942583732057, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 23250; Mature: 23118

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATLFFDLDGTLVDSEPGIVASIVYAFDELGQPRPSAQTLRAWIGPPLRDSFAECFAN
CCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCC
DPGRGQRALGLYRARYDAVGWTELSVFDGIGEVVASLQRAGHRLAVVTSKNERYARRIVE
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
HLPFGACFEEVIGASEDGERRFKPDLIAEALRRLQIEKTGCVMIGDRRMDIDGANHHGIH
HCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCE
SIGVLWGFGDEAELRAAGAGALARTPADLVALLQ
EEEEEECCCCCHHHHHCCCCCHHCCHHHHHHHCC
>Mature Secondary Structure 
SAATLFFDLDGTLVDSEPGIVASIVYAFDELGQPRPSAQTLRAWIGPPLRDSFAECFAN
CCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCC
DPGRGQRALGLYRARYDAVGWTELSVFDGIGEVVASLQRAGHRLAVVTSKNERYARRIVE
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
HLPFGACFEEVIGASEDGERRFKPDLIAEALRRLQIEKTGCVMIGDRRMDIDGANHHGIH
HCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCE
SIGVLWGFGDEAELRAAGAGALARTPADLVALLQ
EEEEEECCCCCHHHHHCCCCCHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]