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The map label for this gene is pgk [H]

Identifier: 84624872

GI number: 84624872

Start: 3645522

End: 3646697

Strand: Reverse

Name: pgk [H]

Synonym: XOO_3215

Alternate gene names: 84624872

Gene position: 3646697-3645522 (Counterclockwise)

Preceding gene: 84624873

Following gene: 84624871

Centisome position: 73.82

GC content: 65.56

Gene sequence:

>1176_bases
ATGTCCATTGTCCGCATGACCGACCTCGATCTCTCCGGCAAGCGCGTGCTGATCCGCCAGGATCTCAACGTGCCGATCGA
CAACGGCCAGATCACCTCCGAGCAGCGCATCACCGCCTCGGTGCCCACCATCAAGCTGGCGTTGGAAAAGGGCGCGGCCG
TCATGGTCACCTCGCACCTGGGTCGCCCCAAGGAAGGCAGCTGGACCGAAGAAGATTCGCTGGCGCCGGTGGCCACGCGT
CTGGCTGCGTTGCTGGGCGTGGACGTGCCGCTGGTCCGCGACTGGGTCGACGGCGTGGAGGTCGCACCGGGCCAGGTGGT
GCTGCTGGAAAATTGCCGCATGAATGTCGGCGAGGGCAAGGACGACCAGACGCTGGCGCGCAAGTACGCCGCGTTGTGCG
ACGTGTTCGTGATGGATGCCTTCGGCACCGCGCACCGCGCGCAGGCCTCCACGCATGGCGTGATCCGCTTCGCGCCGGTC
GCAGCAGGCGGCCCGCTGTTGATGGCCGAACTCGACGCACTGGCCAAGGCGCTGGACAACCCGGCCAAGCCGCTGCTGGC
GATCGTGGCCGGCAGCAAGGTCTCCACCAAGCTGGAGCTGCTGTCCAACCTGGTCAACAAGGTCGACCAGCTGATCGTTG
GCGGCGGCATCGCCAATACCTTCATCGCTGCAGCCGGACACCACGTGGGCAAGTCGTTGAACGAGCCGGACTTGATCCCG
ACCGCCAACCAGATCGTGGCCGACGCCAAGACGCGTGGCGCAGAAATTCCGCTGCCCACCGATGTGGTCGTTGCCAAGCA
GTTCCTGCCCGACGCCGAGGCCAGCGTGAAGGCGTTGGATGCGGTCGATGCCGACGATCTGATCCTGGATATCGGCCCGC
AAACCGCGCAGCGCTATGCCGAGCTGATCGCCAGCGCCGGCACGGTGGTGTGGAACGGGCCGGTCGGCGTGTTCGAATTC
GAGTCCTTCAGCCACGGCACCGAAACCTTGGCGCGCGCGATCGCCAGCTCCAAGGCGTTTTCGATTGCCGGTGGTGGCGA
CACCTTGGCCGCAGTGGACAAGTACGACATCGCCAAGGACGTCACCTACATTTCCACTGGCGGCGGTGCATTCCTGGAGT
TTCTGGAAGGCAAGACCTTGCCGGCTGTGGCCGCACTGCAGGCCCGCGGTCAGTGA

Upstream 100 bases:

>100_bases
CGTTACGGCGCAGCGCTCAGACGCTTGCCACGGCACGCATGCTGCGTCTTCTGCATGACCAATTCCCCATCCCCCTCCCT
AGACCGAACGAGCTGAGCCC

Downstream 100 bases:

>100_bases
GTGCAGCCACGCTGTTTTTCGATCTGGACGGCACGCTGGTCGATTCGGAGCCCGGCATCGTCGCCAGCATCGTGTATGCC
TTCGACGAACTCGGGCAGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATR
LAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDQTLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPV
AAGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHHVGKSLNEPDLIP
TANQIVADAKTRGAEIPLPTDVVVAKQFLPDAEASVKALDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEF
ESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAKDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ

Sequences:

>Translated_391_residues
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATR
LAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDQTLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPV
AAGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHHVGKSLNEPDLIP
TANQIVADAKTRGAEIPLPTDVVVAKQFLPDAEASVKALDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEF
ESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAKDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ
>Mature_390_residues
SIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATRL
AALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDQTLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVA
AGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHHVGKSLNEPDLIPT
ANQIVADAKTRGAEIPLPTDVVVAKQFLPDAEASVKALDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEFE
SFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAKDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=42.0537897310513, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=42.5427872860636, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=62.6598465473146, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17508823, Length=406, Percent_Identity=45.320197044335, Blast_Score=306, Evalue=8e-84,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=42.7536231884058, Blast_Score=288, Evalue=7e-79,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=43.3414043583535, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24581203, Length=411, Percent_Identity=40.8759124087591, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40841; Mature: 40710

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHL
CCEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCEEEEEECCCCEEEEEECC
GRPKEGSWTEEDSLAPVATRLAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
DDQTLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVAAGGPLLMAELDALAKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
PAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHHVGKSLNEPDLIP
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
TANQIVADAKTRGAEIPLPTDVVVAKQFLPDAEASVKALDAVDADDLILDIGPQTAQRYA
CHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHH
ELIASAGTVVWNGPVGVFEFESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAKD
HHHHHCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHC
VTYISTGGGAFLEFLEGKTLPAVAALQARGQ
CEEEECCCHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHL
CEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCEEEEEECCCCEEEEEECC
GRPKEGSWTEEDSLAPVATRLAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
DDQTLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVAAGGPLLMAELDALAKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
PAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHHVGKSLNEPDLIP
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
TANQIVADAKTRGAEIPLPTDVVVAKQFLPDAEASVKALDAVDADDLILDIGPQTAQRYA
CHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHH
ELIASAGTVVWNGPVGVFEFESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAKD
HHHHHCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHC
VTYISTGGGAFLEFLEGKTLPAVAALQARGQ
CEEEECCCHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]