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The map label for this gene is pykA [H]

Identifier: 84624870

GI number: 84624870

Start: 3643158

End: 3644624

Strand: Reverse

Name: pykA [H]

Synonym: XOO_3213

Alternate gene names: 84624870

Gene position: 3644624-3643158 (Counterclockwise)

Preceding gene: 84624871

Following gene: 84624869

Centisome position: 73.77

GC content: 64.83

Gene sequence:

>1467_bases
ATGAAAGAACGTCAACGCCGTACCAAAATTCTCGCCACCCTCGGACCGTCAACGGATGCGCCCGGTGTGCTGGATACGTT
GTTCAAGGCGGGCGTCAACGTGGTGCGCCTCAACTTCAGCCACGGCGACCCGTCCGGGCAGGCCAAGCGTGCTGCCGAAG
TGCGTGCCGCTGCGGCCCGCGTCGGCGCCGAAGTCGGCATCCTTGCCGACTTGCCGGGTCCGAAGATCCGCATCGAGCGT
TTTGCCGAAGGCAAGATCAAGCTGACCATGGGCGATCGCTTCGACCTGGTGGCCGATGCCAATGCGCCGGCCGGCGACCA
GCACCAGGTCGGCGTCAGCTACCTGGGCCTGCCGCAGGACGTGACTGCTGGCGACGTGCTGCTGCTCGACGATGGCCTGA
TGCAACTGCAGGTCACCGACGTGCAGGGCGCGCGCATCGTTACCACCGTGCTCAACGATGGCGTGTTGTCCGACCGCAAG
GGCCTCAACAAGCAGGGCGGCGGCTTGTCGCTCGGCGCGTTGACCGAGCGCGACAAAGAGCTGATCGGTATCGTCGCCAA
GATCGGCGTGGATTTCATCGCCGTGTCGTTCTGTCGCAATGCGCAGGACATGCAAGACGCCCGTCAGATCGCCAAGCAGC
ACGGCTGCGACGCGCAGCTGGTGTCGAAGATCGAGCGCACCGAAGCGATCGAAAACCTGGTCGAGATCGTCGAAGCCTCC
GATGTGGTGATGGTGGCGCGTGGCGACCTGGGCGTTGAAATCGGCGATGCCGAATTGCCCGGGCTGCAGAAGAAGATCAT
TCGCGAATCGTTGGCGCAGAACAAAGTGGTGATCACCGCCACGCAGATGCTGCAGTCGATGGTCGAGAGCCCGATCCCGA
CCCGCGCCGAAGTGCTGGACGTGGCCAATGCGGTCATCGACGGCACCGATGCGGTGATGCTCTCGGCCGAAACCGCCGCC
GGTGCCTATCCGGTGCGTGCGGTGGAAGCGATGGCGCGTATCTGCCTGGGTGCCGAGCACCAGTTCGAATTCGATACCGA
CTTCGAAGCGGCGCAGCGTAATCTGCAGCGCGCCGACCAGGCCATCGCGATGGCGACCATGTTCCTGTCCGAGCACATCG
GTCTGGGCGGCGTGGTGGCGTTGACCGAATCCGGCGGTACCCCGCGCTTTCTGTCGCGCTTCCGCTCCAACATGCCGATC
TATGCGTTCACCCGTCACGATGGCGCGCGTCGCCAGATGGCGATGATGCGTGGCGTGTTCCCGATCAACTTCGACAGCCG
CGGCCTGACCCCGCGCGAAGCCGCACGTGCGGCGATCCGTCTGTTGGTGGAGAACGAGCGCATGGGCCCGGGCGACCGCG
TGGTGTTTACCAGCGGCGAGCACATGGAAACGCACGGCGCCACCAACACGCTGCGTTTGCTGGAAGTGGGCGACGACGGC
CGCGCTACCGGTCTGGGCGAGCTGTAA

Upstream 100 bases:

>100_bases
TCTGTAAGACATGCCGGAATCGGAGGATGGCTATGCCGCAATGCCATCCTTGGTGCGTGACGTGTGTGTTAATTTTCGCG
GTTTTTATAGGGAGGCCATC

Downstream 100 bases:

>100_bases
CGTCCGTTGACCCCCTCCGGCGCACAGCCGAGCCGGAAGACGTGACACGCAGCGGCGACCCCAGGGTCGCCGTTGTGCTG
TGTGGCGATGCCGGGGGCGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKERQRRTKILATLGPSTDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQHQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGARIVTTVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMQDARQIAKQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPINFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGDDG
RATGLGEL

Sequences:

>Translated_488_residues
MKERQRRTKILATLGPSTDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQHQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGARIVTTVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMQDARQIAKQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPINFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGDDG
RATGLGEL
>Mature_488_residues
MKERQRRTKILATLGPSTDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQHQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGARIVTTVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMQDARQIAKQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPINFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGDDG
RATGLGEL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=430, Percent_Identity=36.046511627907, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI33286420, Length=430, Percent_Identity=36.046511627907, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI33286418, Length=430, Percent_Identity=35.8139534883721, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=35.0912778904665, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=35.0912778904665, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=33.4494773519164, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=33.4494773519164, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=30.6382978723404, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=30.6382978723404, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.3738317757009, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=51.0416666666667, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=37.9014989293362, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=34.5755693581781, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=35.5102040816327, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=35.5102040816327, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=35.5102040816327, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=35.5102040816327, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=434, Percent_Identity=35.9447004608295, Blast_Score=256, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=34.5528455284553, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=35.8606557377049, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=35.8606557377049, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=35.2216748768473, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24581235, Length=430, Percent_Identity=30.6976744186047, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24646914, Length=322, Percent_Identity=34.472049689441, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52298; Mature: 52298

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERQRRTKILATLGPSTDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAAR
CCCHHHHHHEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
VGAEVGILADLPGPKIRIERFAEGKIKLTMGDRFDLVADANAPAGDQHQVGVSYLGLPQD
HCCCEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEECCCCC
VTAGDVLLLDDGLMQLQVTDVQGARIVTTVLNDGVLSDRKGLNKQGGGLSLGALTERDKE
CCCCCEEEEECCEEEEEEECCCCCEEEEEHHCCCCCCCCCCCCCCCCCEEECCCCHHHHH
LIGIVAKIGVDFIAVSFCRNAQDMQDARQIAKQHGCDAQLVSKIERTEAIENLVEIVEAS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETAAGAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPIYAFTRHDGARRQMAMMRGVF
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PINFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGDDG
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCCEEEEEEECCCC
RATGLGEL
CCCCCCCC
>Mature Secondary Structure
MKERQRRTKILATLGPSTDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAAR
CCCHHHHHHEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
VGAEVGILADLPGPKIRIERFAEGKIKLTMGDRFDLVADANAPAGDQHQVGVSYLGLPQD
HCCCEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEECCCCC
VTAGDVLLLDDGLMQLQVTDVQGARIVTTVLNDGVLSDRKGLNKQGGGLSLGALTERDKE
CCCCCEEEEECCEEEEEEECCCCCEEEEEHHCCCCCCCCCCCCCCCCCEEECCCCHHHHH
LIGIVAKIGVDFIAVSFCRNAQDMQDARQIAKQHGCDAQLVSKIERTEAIENLVEIVEAS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETAAGAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPIYAFTRHDGARRQMAMMRGVF
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PINFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGDDG
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCCEEEEEEECCCC
RATGLGEL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]