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The map label for this gene is pglA [H]

Identifier: 84624202

GI number: 84624202

Start: 2836772

End: 2838442

Strand: Direct

Name: pglA [H]

Synonym: XOO_2545

Alternate gene names: 84624202

Gene position: 2836772-2838442 (Clockwise)

Preceding gene: 229258271

Following gene: 84624209

Centisome position: 57.42

GC content: 60.56

Gene sequence:

>1671_bases
ATGCAGTCCCATCGCTTTGTTCCAACCGTCACGCCACTGACCCTCGCCCTGCTCGGTCTGGCTACGTTCCACGCCAGCGC
TGCACGACCGCACGGGCAGTACGTACCTGATACCGCGCACAGCATTTCCACAAGCTGGGGGGTGGTCCAGCAGCCCACAT
TACCGACCCAGGTATGCGCCACGCTCAAGGCCGCGCTGATGCCGGTGGGCGGCTCGCTCGATACGCTTGACCAGAATCCG
GCACACTCCAAACGCGACACCGCGCGCTTGCAGGCGGCCATCGACCATTGCCCTGCCGGCAGCGCAGTGCACCTTGTCCC
CGGCGATGCCGGCGAATCCGGGTTGCTGACCGGGCCGCTGACCATTAAAAGCGGTGTCACGCTATGGATCGACGGCGGCG
TCACCTTGTTCGGCTCGCGCAATCCGCTGGATTACGACAACGGCCTGGGCACCTGCGGCACTGCGACCAGCGACAAGACG
AAGTCATGCAAGCCGCTCATCCACGTCGCCGATACCGCAAAAAGCGCGATCGTTGGTGCGGGCAAGATCGATGGACGCGG
CGGCAGCACGCTGACTGCCGGGCCAAACGCCGGCACCGCCAGTTGGTGGGATCTGGCCTATCTCAACGTCACTAAAGGCC
TGAGCCAACACGTCCCGCGGCTGCTGCAGATCGACGACAGCACTGACTTCACGTTGTACGACATCACTCTGGAAAACTCG
GCGAATTTCCATGTGACCACCGATAACGTGGTTGGCCTGACGGCGTGGGGCATCAAAATTCTTGCGCCCAGCCTGGTCTA
CTCGCGGCCCGGCTACCACTGCCCGGCCGGCAGCACGCCTGATGTCAATCCGCACGCCACCTGCTTCACCCCGAAAACCG
CCAAGAATACCGATGGTTTCGATCCGGGCCAGTCGAAGAATGTACTGCTGACGTATTCGTACATCGCTACCGGCGATGAC
GGTGTTGCGATCAAGGCACATGCCAGTAGCAAGCGCAGCATCGCATCGGAAAACATGCTGTTCACCTACAACCAGTTCTA
TTACACACATGGTTTTTCATTGGGCAGCGAAACCGACTCGGGCATGCGCCATATCGCCGTGCGCGGGCTGAGCATCGATG
GCTTCAATAGCAACGATGTACACGACGACCCGTATTCTTCCAACGGGCTGCGGATCAAATCCGATGGCACGCGCGGCGGC
CAGGTCTACGACATCAGTTTTGAAAACATCTGCATGCGCGGTGTGGCAAGGCCGCTGGTGTTCGATGCGAACTACGCCAA
TGCAGCAGTGCGCAGCAAACTGCCGAGTTTCAGCGGCATAACGCTGACCAATGTGCACTCGCTGGGTTCCAAGGCATTCG
GCGGCGGCGAGCTGAGCTTCTACGGCTACCGCGATGCGAAGACCACCTTGCCGATCAGCATTTCGCTGGACAACGTGGTG
CTGGAAGGTGGAAAGGTCTCGTTCGCAAAGCCGCATTTCGGCGGGCCTGCGAGCAACCCGGGCACGACGCATTTCACGTT
TAAAGGCGGTCCAGTGAGCTTTTTCGATCAGCTGACCGAATCAGCGTCCAACGATGTGCAGCTGCAAGGGAAGCCTGGCC
CTGGCGTGCCGTTGCAATGCAACGACGCCTTCATCGCCTACCACTCGGTGCTGCCGGATTCGCCGATCTGA

Upstream 100 bases:

>100_bases
GTGCGTGCGCGTCGCAGGATCATCGCATCACGCACACGCTGGTATCTCGCTGCCGCTCGCTGATCTGGCACGTCTGCCGC
ATTCCACCGGACCAACACCC

Downstream 100 bases:

>100_bases
GCACAGCTGATTGAGACACCGCGCTTGCCACACGGCAAGCGCGGATGCCGAGCGCGGTCAGCGTACTTGCTGCGCCTAAA
GCGCCTAAAAACACGACTGC

Product: polygalacturonase

Products: NA

Alternate protein names: PGA; Pectinase [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MQSHRFVPTVTPLTLALLGLATFHASAARPHGQYVPDTAHSISTSWGVVQQPTLPTQVCATLKAALMPVGGSLDTLDQNP
AHSKRDTARLQAAIDHCPAGSAVHLVPGDAGESGLLTGPLTIKSGVTLWIDGGVTLFGSRNPLDYDNGLGTCGTATSDKT
KSCKPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGTASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
ANFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHATCFTPKTAKNTDGFDPGQSKNVLLTYSYIATGDD
GVAIKAHASSKRSIASENMLFTYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNSNDVHDDPYSSNGLRIKSDGTRGG
QVYDISFENICMRGVARPLVFDANYANAAVRSKLPSFSGITLTNVHSLGSKAFGGGELSFYGYRDAKTTLPISISLDNVV
LEGGKVSFAKPHFGGPASNPGTTHFTFKGGPVSFFDQLTESASNDVQLQGKPGPGVPLQCNDAFIAYHSVLPDSPI

Sequences:

>Translated_556_residues
MQSHRFVPTVTPLTLALLGLATFHASAARPHGQYVPDTAHSISTSWGVVQQPTLPTQVCATLKAALMPVGGSLDTLDQNP
AHSKRDTARLQAAIDHCPAGSAVHLVPGDAGESGLLTGPLTIKSGVTLWIDGGVTLFGSRNPLDYDNGLGTCGTATSDKT
KSCKPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGTASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
ANFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHATCFTPKTAKNTDGFDPGQSKNVLLTYSYIATGDD
GVAIKAHASSKRSIASENMLFTYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNSNDVHDDPYSSNGLRIKSDGTRGG
QVYDISFENICMRGVARPLVFDANYANAAVRSKLPSFSGITLTNVHSLGSKAFGGGELSFYGYRDAKTTLPISISLDNVV
LEGGKVSFAKPHFGGPASNPGTTHFTFKGGPVSFFDQLTESASNDVQLQGKPGPGVPLQCNDAFIAYHSVLPDSPI
>Mature_556_residues
MQSHRFVPTVTPLTLALLGLATFHASAARPHGQYVPDTAHSISTSWGVVQQPTLPTQVCATLKAALMPVGGSLDTLDQNP
AHSKRDTARLQAAIDHCPAGSAVHLVPGDAGESGLLTGPLTIKSGVTLWIDGGVTLFGSRNPLDYDNGLGTCGTATSDKT
KSCKPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGTASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
ANFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHATCFTPKTAKNTDGFDPGQSKNVLLTYSYIATGDD
GVAIKAHASSKRSIASENMLFTYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNSNDVHDDPYSSNGLRIKSDGTRGG
QVYDISFENICMRGVARPLVFDANYANAAVRSKLPSFSGITLTNVHSLGSKAFGGGELSFYGYRDAKTTLPISISLDNVV
LEGGKVSFAKPHFGGPASNPGTTHFTFKGGPVSFFDQLTESASNDVQLQGKPGPGVPLQCNDAFIAYHSVLPDSPI

Specific function: Contributes to the wilt disease production on tomato [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 58575; Mature: 58575

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSHRFVPTVTPLTLALLGLATFHASAARPHGQYVPDTAHSISTSWGVVQQPTLPTQVCA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCHHHHHH
TLKAALMPVGGSLDTLDQNPAHSKRDTARLQAAIDHCPAGSAVHLVPGDAGESGLLTGPL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCE
TIKSGVTLWIDGGVTLFGSRNPLDYDNGLGTCGTATSDKTKSCKPLIHVADTAKSAIVGA
EECCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEC
GKIDGRGGSTLTAGPNAGTASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
CEECCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEECCCCCEEEEEEEECCC
ANFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHATCFTPKTAKNTDGF
CCEEEECCCEEEEEECCCEECCCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DPGQSKNVLLTYSYIATGDDGVAIKAHASSKRSIASENMLFTYNQFYYTHGFSLGSETDS
CCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC
GMRHIAVRGLSIDGFNSNDVHDDPYSSNGLRIKSDGTRGGQVYDISFENICMRGVARPLV
CCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECHHHHHHHHCCCCEE
FDANYANAAVRSKLPSFSGITLTNVHSLGSKAFGGGELSFYGYRDAKTTLPISISLDNVV
EECCCCCHHHHHCCCCCCCEEEEEHHHCCCCCCCCCCEEEEEECCCCEEEEEEEEECCEE
LEGGKVSFAKPHFGGPASNPGTTHFTFKGGPVSFFDQLTESASNDVQLQGKPGPGVPLQC
EECCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEE
NDAFIAYHSVLPDSPI
CCCEEEEEECCCCCCC
>Mature Secondary Structure
MQSHRFVPTVTPLTLALLGLATFHASAARPHGQYVPDTAHSISTSWGVVQQPTLPTQVCA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCHHHHHH
TLKAALMPVGGSLDTLDQNPAHSKRDTARLQAAIDHCPAGSAVHLVPGDAGESGLLTGPL
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCE
TIKSGVTLWIDGGVTLFGSRNPLDYDNGLGTCGTATSDKTKSCKPLIHVADTAKSAIVGA
EECCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEC
GKIDGRGGSTLTAGPNAGTASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
CEECCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEECCCCCEEEEEEEECCC
ANFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHATCFTPKTAKNTDGF
CCEEEECCCEEEEEECCCEECCCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DPGQSKNVLLTYSYIATGDDGVAIKAHASSKRSIASENMLFTYNQFYYTHGFSLGSETDS
CCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC
GMRHIAVRGLSIDGFNSNDVHDDPYSSNGLRIKSDGTRGGQVYDISFENICMRGVARPLV
CCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECHHHHHHHHCCCCEE
FDANYANAAVRSKLPSFSGITLTNVHSLGSKAFGGGELSFYGYRDAKTTLPISISLDNVV
EECCCCCHHHHHCCCCCCCEEEEEHHHCCCCCCCCCCEEEEEECCCCEEEEEEEEECCEE
LEGGKVSFAKPHFGGPASNPGTTHFTFKGGPVSFFDQLTESASNDVQLQGKPGPGVPLQC
EECCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEE
NDAFIAYHSVLPDSPI
CCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193922 [H]