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The map label for this gene is pme [H]

Identifier: 229258271

GI number: 229258271

Start: 2833960

End: 2835183

Strand: Direct

Name: pme [H]

Synonym: XOO_2543

Alternate gene names: 229258271

Gene position: 2833960-2835183 (Clockwise)

Preceding gene: 84624199

Following gene: 84624202

Centisome position: 57.37

GC content: 62.09

Gene sequence:

>1224_bases
TTGACCTGCCCCACACGTCCCTTCCTGAGAGAGCGCCACCACATGTCTGAATCGCACATCCGTACGACCTGCCTCGCCAC
CGCACTGTTGAGCCTTGTCAGCGCCAATGCGATGGCATTGACCGGCACCGCCACGCGACCGCAACTCACCACTAGCGAAG
CAAGCACCTACACCATCACAAAAGCCTTGGCCAAGGCCGGACCGATCACTGCGCTGGTGACCGACAATTGGAATCCCACG
GCAGGCGTTGCCTTGCTCAGCGCCGATTACGCAGTGGCTGCCGACGGCTCGACACCCTACCGCACCGTGCAGGCGGCTAT
TGATGCGGCGGTTGCTGCAGGCGGCACCACACGTCGCTACATCAGCATCAAGGCGGGCACCTACACCGGACTGGTGTGCG
TGCCCACCAATGCGCCACCACTGACGGTGTTCGGGCTGGGCACCGACAGCACAGATACGGTCATCCGCTTCAACAATGCC
AACCCCACTCCCAAACCGACTGGCATCGCAAGCCACCCTTGCGCAAGCAATGCAGCGGCAACCACGGTGGGCACGTCCAA
TAGCGCTACGTTGACGGTGCGCGCAGCAGGCTTCCAGGCGCGTCACCTGCGCGTGGACAACGACTATGTCGAAGGCACTT
ACACAGACAATAACCAGTCGGCTGTCGCGCTGGCAGTGCGTGGTGACAAAGCCAGTTTCGAAGACGTCGTGATCACCGGT
AACCAGGACACCTTGTTGATCAGCGCGACCAACGCGGCCAATGTGATCCGCTCTTACTTCAAGAACAGCACAATCGAGGG
CGACGTCGATTTCATCTTCGGCTCGGGTGTCGGCGTGTTCGACAATGCCCTCATCCGCTCCGCTGGCGCGCGCCTGGGCA
GCAGCAGCGGCGGCTACATCTTCGCGCCCAGTACGCGCCCGGGCAGTCCCTATGGTTTTCTCGCGATCAATAGCCGCTTT
GTGGCTGGCTCAGGCTCGCCCGACAACCAGACCTACCTTGGGCGTGCCTGGGACGAAGGCGTCCGCGGCCTGTCGGCCTA
CGTCAACGGCACCTCGCCCAATGGGCAAGTGACAGTGCGCGATTCCAACCTGGGCAGCCATATTCGCAAGACCGCGCCGT
GGGATGCGTCCACCGCCAGCCGCCCGTATTGCAGCAGCAATTGCACCAATTCGGCGAACCGCTTTTATGAGTATGGGAAC
AGCGGCGCCGGCGCTGGGAATTAG

Upstream 100 bases:

>100_bases
GCACAGCCATCGCAGCACTAAAAGCCGTGTTCGATGCCCGCTTCGGGCGCACGTGTCAAGCCTGCGGTAGAGGCACGCAT
CATCACATTGCACAAGCGTA

Downstream 100 bases:

>100_bases
ACCGTCGCTGTGCGCTGACTGCGCAACAAGCCAGGCACGACCTGTGCTTGCTGCGTGTGATGCAGTGTTAGAGATGCACC
TTCTTGATCTTGTGCGCTCC

Product: putative pectinesterase

Products: NA

Alternate protein names: PE; Pectin methylesterase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTCPTRPFLRERHHMSESHIRTTCLATALLSLVSANAMALTGTATRPQLTTSEASTYTITKALAKAGPITALVTDNWNPT
AGVALLSADYAVAADGSTPYRTVQAAIDAAVAAGGTTRRYISIKAGTYTGLVCVPTNAPPLTVFGLGTDSTDTVIRFNNA
NPTPKPTGIASHPCASNAAATTVGTSNSATLTVRAAGFQARHLRVDNDYVEGTYTDNNQSAVALAVRGDKASFEDVVITG
NQDTLLISATNAANVIRSYFKNSTIEGDVDFIFGSGVGVFDNALIRSAGARLGSSSGGYIFAPSTRPGSPYGFLAINSRF
VAGSGSPDNQTYLGRAWDEGVRGLSAYVNGTSPNGQVTVRDSNLGSHIRKTAPWDASTASRPYCSSNCTNSANRFYEYGN
SGAGAGN

Sequences:

>Translated_407_residues
MTCPTRPFLRERHHMSESHIRTTCLATALLSLVSANAMALTGTATRPQLTTSEASTYTITKALAKAGPITALVTDNWNPT
AGVALLSADYAVAADGSTPYRTVQAAIDAAVAAGGTTRRYISIKAGTYTGLVCVPTNAPPLTVFGLGTDSTDTVIRFNNA
NPTPKPTGIASHPCASNAAATTVGTSNSATLTVRAAGFQARHLRVDNDYVEGTYTDNNQSAVALAVRGDKASFEDVVITG
NQDTLLISATNAANVIRSYFKNSTIEGDVDFIFGSGVGVFDNALIRSAGARLGSSSGGYIFAPSTRPGSPYGFLAINSRF
VAGSGSPDNQTYLGRAWDEGVRGLSAYVNGTSPNGQVTVRDSNLGSHIRKTAPWDASTASRPYCSSNCTNSANRFYEYGN
SGAGAGN
>Mature_406_residues
TCPTRPFLRERHHMSESHIRTTCLATALLSLVSANAMALTGTATRPQLTTSEASTYTITKALAKAGPITALVTDNWNPTA
GVALLSADYAVAADGSTPYRTVQAAIDAAVAAGGTTRRYISIKAGTYTGLVCVPTNAPPLTVFGLGTDSTDTVIRFNNAN
PTPKPTGIASHPCASNAAATTVGTSNSATLTVRAAGFQARHLRVDNDYVEGTYTDNNQSAVALAVRGDKASFEDVVITGN
QDTLLISATNAANVIRSYFKNSTIEGDVDFIFGSGVGVFDNALIRSAGARLGSSSGGYIFAPSTRPGSPYGFLAINSRFV
AGSGSPDNQTYLGRAWDEGVRGLSAYVNGTSPNGQVTVRDSNLGSHIRKTAPWDASTASRPYCSSNCTNSANRFYEYGNS
GAGAGN

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

Organism=Escherichia coli, GI1786989, Length=407, Percent_Identity=31.2039312039312, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 42284; Mature: 42153

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCPTRPFLRERHHMSESHIRTTCLATALLSLVSANAMALTGTATRPQLTTSEASTYTIT
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCEEHHH
KALAKAGPITALVTDNWNPTAGVALLSADYAVAADGSTPYRTVQAAIDAAVAAGGTTRRY
HHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
ISIKAGTYTGLVCVPTNAPPLTVFGLGTDSTDTVIRFNNANPTPKPTGIASHPCASNAAA
EEEECCCEEEEEEECCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCE
TTVGTSNSATLTVRAAGFQARHLRVDNDYVEGTYTDNNQSAVALAVRGDKASFEDVVITG
EEECCCCCEEEEEEECCCEEEEEEECCCEECCEEECCCCCEEEEEEECCCCCCCCEEEEC
NQDTLLISATNAANVIRSYFKNSTIEGDVDFIFGSGVGVFDNALIRSAGARLGSSSGGYI
CCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEE
FAPSTRPGSPYGFLAINSRFVAGSGSPDNQTYLGRAWDEGVRGLSAYVNGTSPNGQVTVR
ECCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCHHHHHCEEEEEECCCCCCCEEEEE
DSNLGSHIRKTAPWDASTASRPYCSSNCTNSANRFYEYGNSGAGAGN
ECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TCPTRPFLRERHHMSESHIRTTCLATALLSLVSANAMALTGTATRPQLTTSEASTYTIT
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCEEHHH
KALAKAGPITALVTDNWNPTAGVALLSADYAVAADGSTPYRTVQAAIDAAVAAGGTTRRY
HHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
ISIKAGTYTGLVCVPTNAPPLTVFGLGTDSTDTVIRFNNANPTPKPTGIASHPCASNAAA
EEEECCCEEEEEEECCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCE
TTVGTSNSATLTVRAAGFQARHLRVDNDYVEGTYTDNNQSAVALAVRGDKASFEDVVITG
EEECCCCCEEEEEEECCCEEEEEEECCCEECCEEECCCCCEEEEEEECCCCCCCCEEEEC
NQDTLLISATNAANVIRSYFKNSTIEGDVDFIFGSGVGVFDNALIRSAGARLGSSSGGYI
CCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEE
FAPSTRPGSPYGFLAINSRFVAGSGSPDNQTYLGRAWDEGVRGLSAYVNGTSPNGQVTVR
ECCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCHHHHHCEEEEEECCCCCCCEEEEE
DSNLGSHIRKTAPWDASTASRPYCSSNCTNSANRFYEYGNSGAGAGN
ECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]