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The map label for this gene is hisF [H]

Identifier: 84623781

GI number: 84623781

Start: 2350214

End: 2350990

Strand: Direct

Name: hisF [H]

Synonym: XOO_2124

Alternate gene names: 84623781

Gene position: 2350214-2350990 (Clockwise)

Preceding gene: 84623780

Following gene: 84623782

Centisome position: 47.57

GC content: 62.93

Gene sequence:

>777_bases
ATGCTGAGCCGTCGCATTATTCCGTGCCTGGACGTGCGCAATGGCCGCGTGGTCAAGGGCGTCAAGTTCCACGACCACAT
CGACATGGGCGACATCGTCGAACTGGCCTTGCGCTACCGCGCGCAGGGCGCCGACGAATTGGTGTTCTACGACATCGGTG
CCAGCCCGGAAGGCCGTTCGGTCGACTACACCTGGGTCGAGCGGGTGGCGCGGTTGATCGATATTCCGTTCTGCGTGGCC
GGAGGTATTGGCGATGTGGAAACGGCGCGTGCGGTGCTACACGCCGGTGCCGACAAGATATCGATCAACTCACCCGCCTT
GGGCCGCCCGCAATTGATTTCCGAGCTGGCCGATGCCTTCGGCGTGCAATGCGTGGTGGTCGGCATCGATTCGATCCGTG
AGGACGATGGCCAGTGGCGCGTGCGCCGCTACACCGGCGATCCGAGCAAGACCCAGGCGCTGCCGATGCGCACGCTGGAC
TGGGTGGCCGAAGCGCAGCGGCTCGGCGCGGGCGAAATCGTGCTCAATTGCATGGACAACGACGGCGTACGCCGTGGCTA
CGACATTGCCCAGCTGCGGCAGGTGCGCGCGTTATGCCATGTGCCCTTGATCGCCTCGGGGGGTGCGGGTGAGATGCAGC
ATTTTGCCGATGTATTCGACCAGGCTGATGTGGATGGCGCACTGGCAGCGAGCGTGTTTCATAGCGGTGCGATTCCGATT
CCGGAACTCAAGCAATTTTTGCGCGCGCAACAGATCGAGGTACGCGATGGGCAGTAA

Upstream 100 bases:

>100_bases
ATCTGGCCGACGTCGCCGCTGCCAAGGCCGCAGGTTGCGCCGGCATCGTGCTTGGCAAGGCCTTGCTGGAAGGGCATCTG
GATCTCGACGAGGCACTGGC

Downstream 100 bases:

>100_bases
CGACGTGGCAACGGGCGACGCGCTCGTAACGTTGGATTGGAGCAAGGGCGACGGCCTGTTGCCGGTGATCGTGCAGGACG
CCGACAATTTGCGCGTGTTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLSRRIIPCLDVRNGRVVKGVKFHDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREDDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEIVLNCMDNDGVRRGYDIAQLRQVRALCHVPLIASGGAGEMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ

Sequences:

>Translated_258_residues
MLSRRIIPCLDVRNGRVVKGVKFHDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREDDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEIVLNCMDNDGVRRGYDIAQLRQVRALCHVPLIASGGAGEMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ
>Mature_258_residues
MLSRRIIPCLDVRNGRVVKGVKFHDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRSVDYTWVERVARLIDIPFCVA
GGIGDVETARAVLHAGADKISINSPALGRPQLISELADAFGVQCVVVGIDSIREDDGQWRVRRYTGDPSKTQALPMRTLD
WVAEAQRLGAGEIVLNCMDNDGVRRGYDIAQLRQVRALCHVPLIASGGAGEMQHFADVFDQADVDGALAASVFHSGAIPI
PELKQFLRAQQIEVRDGQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=63.671875, Blast_Score=328, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=30.3514376996805, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28201; Mature: 28201

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRIIPCLDVRNGRVVKGVKFHDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRS
CCCCCCCCEEECCCCEEEECEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYTWVERVARLIDIPFCVAGGIGDVETARAVLHAGADKISINSPALGRPQLISELADAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREDDGQWRVRRYTGDPSKTQALPMRTLDWVAEAQRLGAGEIVLNCMDN
CCEEEEEECHHHCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
DGVRRGYDIAQLRQVRALCHVPLIASGGAGEMQHFADVFDQADVDGALAASVFHSGAIPI
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
PELKQFLRAQQIEVRDGQ
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSRRIIPCLDVRNGRVVKGVKFHDHIDMGDIVELALRYRAQGADELVFYDIGASPEGRS
CCCCCCCCEEECCCCEEEECEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
VDYTWVERVARLIDIPFCVAGGIGDVETARAVLHAGADKISINSPALGRPQLISELADAF
CCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDSIREDDGQWRVRRYTGDPSKTQALPMRTLDWVAEAQRLGAGEIVLNCMDN
CCEEEEEECHHHCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
DGVRRGYDIAQLRQVRALCHVPLIASGGAGEMQHFADVFDQADVDGALAASVFHSGAIPI
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
PELKQFLRAQQIEVRDGQ
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]