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The map label for this gene is gltX [H]

Identifier: 84623051

GI number: 84623051

Start: 1520729

End: 1522132

Strand: Direct

Name: gltX [H]

Synonym: XOO_1394

Alternate gene names: 84623051

Gene position: 1520729-1522132 (Clockwise)

Preceding gene: 84623050

Following gene: 84623052

Centisome position: 30.78

GC content: 64.67

Gene sequence:

>1404_bases
ATGGCCTGCCGCACCCGTTTTGCCCCCAGCCCCACCGGTTACCTGCACATCGGCGGCGCGCGCACCGCCCTGTATTGCTG
GCTGGAGGCACGTCGCCGCGGCGGGCAGTTCGTGCTGCGTATCGAAGACACCGACCGCCAGCGCAGCACCCAGGCGGCCA
TCGACGCCATCCTGGAAGCGATGCAGTGGCTGGGCTTGGGTTACGACGAAGGCCCCATCTACCAGACCCAGCGCGTTGCC
CGCTATCAAGAAGTCGCCGAGCAGTTGCTGGCGCAGGGCAAGGCGTATTACGCCTATGAAACCCGCGAAGAGCTCGACGC
CATGCGCGAGGCCGCCATGGCCAAGCAGGAAAAACCGCGTTACGACGGCGCCGCCCGCGAGCAGAACCTGCCGTATCGCG
ACGATCCCAATCGCGTCATCCGTTTCAAGAATCCGATCGGCGGCACGGTGGTGTTCGACGACCTGATCAAGGGCCGCATC
GAGATCGCCAACAGCGAGCTCGACGACATGGTGATCTTCCGTCCGGACGGCCTCCCCACCTACAACTTTGCGGTGGTGGT
GGACGACTGGGACATGGGCATCACCGAGGTGATCCGCGGCGACGACCACATCAACAACACCCCGCGCCAGATCAACATCT
ATGCGGCGTTGGGCGCACCGGTGCCCAAGTTCGCGCACATGCCGATGATTCTGGACGAGCAGGGTACCAAGCTGTCCAAG
CGCACCGGCGCGGCCGACGTCATGCAGTACAAGGACGCCGGCTATCTGCCGCACGCGTTGATCAATTATCTGGCGCGCCT
GGGCTGGTCGCATGGCGACCAGGAGCTGTTCACGCCGCAGGAATTGCTGGACCTGTTCGACGTCAAGGACGTCAATTCCA
AGGCCGCGCGCCTGGACATGGCCAAGCTCGGCTGGGTCAATCAGCATTACCTGAAGACCGACGACCCGGCCAGCATCGCC
CCGCAGCTGGAATACCAGCTTGCCAAGCTCGGCGTGGATCTGGCTGCCGGTCCGGCTGCCGCCGATGTGGTGGTGGCCTT
GCGCGAGCGCGTGCACACCTTGAAGGAAATGGCCGAAAAAGCCGTGGTCTGGTACCAGCCGCTGGAAACCTACGACGCAG
CGGCGGTGATGAAGCACCTCAAGCTGGGTGCCGAAGTGCCGCTTGGCAAAGCACGCGAACTGCTGGCCGCAGTGGACCAG
TGGAGCGTGGACAGCGTCTCGGCCGCGCTGCACGACGCTGCCGCTGCGCTGGAACTGGGCATGGGCAAGGTCGCCCAGCC
GCTGCGCGTGGCCATCACCGGCACCCAGGTCAGCCCGGATATTTCGCAAACGGTGTACCTGGCCGGACGCGAGGGTGCCT
TGAAACGCATCGATGCGGCACTTACCAAGATTGGCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGTGTACGGCGACCTGTTGCGCAACCGCTGAGCCGCGGGCCGGGCAGGGCCTCGCTGCGCAAGGTAAAATCGGCGCACT
CCCGTTTTGTCGAGCGCTCC

Downstream 100 bases:

>100_bases
CGCCGGAGCCCGTATGACCAAGCACACCCACGCACCAGACCTCGCCTGCACCGCACCGCATCACCACGTCGACGACGCCA
ATGGCTTCGTGCGCGCGGTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MACRTRFAPSPTGYLHIGGARTALYCWLEARRRGGQFVLRIEDTDRQRSTQAAIDAILEAMQWLGLGYDEGPIYQTQRVA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYDGAAREQNLPYRDDPNRVIRFKNPIGGTVVFDDLIKGRI
EIANSELDDMVIFRPDGLPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYAALGAPVPKFAHMPMILDEQGTKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTPQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDLAAGPAAADVVVALRERVHTLKEMAEKAVVWYQPLETYDAAAVMKHLKLGAEVPLGKARELLAAVDQ
WSVDSVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALTKIGAA

Sequences:

>Translated_467_residues
MACRTRFAPSPTGYLHIGGARTALYCWLEARRRGGQFVLRIEDTDRQRSTQAAIDAILEAMQWLGLGYDEGPIYQTQRVA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYDGAAREQNLPYRDDPNRVIRFKNPIGGTVVFDDLIKGRI
EIANSELDDMVIFRPDGLPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYAALGAPVPKFAHMPMILDEQGTKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTPQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDLAAGPAAADVVVALRERVHTLKEMAEKAVVWYQPLETYDAAAVMKHLKLGAEVPLGKARELLAAVDQ
WSVDSVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALTKIGAA
>Mature_466_residues
ACRTRFAPSPTGYLHIGGARTALYCWLEARRRGGQFVLRIEDTDRQRSTQAAIDAILEAMQWLGLGYDEGPIYQTQRVAR
YQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRYDGAAREQNLPYRDDPNRVIRFKNPIGGTVVFDDLIKGRIE
IANSELDDMVIFRPDGLPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYAALGAPVPKFAHMPMILDEQGTKLSKR
TGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTPQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIAP
QLEYQLAKLGVDLAAGPAAADVVVALRERVHTLKEMAEKAVVWYQPLETYDAAAVMKHLKLGAEVPLGKARELLAAVDQW
SVDSVSAALHDAAAALELGMGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALTKIGAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=31.1740890688259, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=54.0948275862069, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=39.2561983471074, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=473, Percent_Identity=29.5983086680761, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6324540, Length=503, Percent_Identity=31.2127236580517, Blast_Score=239, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=209, Percent_Identity=28.7081339712919, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=501, Percent_Identity=32.1357285429142, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51649; Mature: 51518

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACRTRFAPSPTGYLHIGGARTALYCWLEARRRGGQFVLRIEDTDRQRSTQAAIDAILEA
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
MQWLGLGYDEGPIYQTQRVARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
YDGAAREQNLPYRDDPNRVIRFKNPIGGTVVFDDLIKGRIEIANSELDDMVIFRPDGLPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHCHHEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYAALGAPVPKFAHMPMILDEQGTKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTPQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDLAAGPAAADVVVALRERVHTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDAAAVMKHLKLGAEVPLGKARELLAAVDQWSVDSVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALTKIGAA
CCHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACRTRFAPSPTGYLHIGGARTALYCWLEARRRGGQFVLRIEDTDRQRSTQAAIDAILEA
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
MQWLGLGYDEGPIYQTQRVARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
YDGAAREQNLPYRDDPNRVIRFKNPIGGTVVFDDLIKGRIEIANSELDDMVIFRPDGLPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHCHHEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYAALGAPVPKFAHMPMILDEQGTKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTPQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDLAAGPAAADVVVALRERVHTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDAAAVMKHLKLGAEVPLGKARELLAAVDQWSVDSVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALTKIGAA
CCHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]