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The map label for this gene is spoT [H]

Identifier: 84622703

GI number: 84622703

Start: 1137508

End: 1139679

Strand: Direct

Name: spoT [H]

Synonym: XOO_1046

Alternate gene names: 84622703

Gene position: 1137508-1139679 (Clockwise)

Preceding gene: 84622702

Following gene: 84622704

Centisome position: 23.03

GC content: 63.58

Gene sequence:

>2172_bases
ATGAACCCAGGCCCTACTGCCCAGGCGACCGTCGTGACGTCTCCGTCTTCCGACCAGGCCATTCCCGACTACGTCCTGCA
CCTCGAACGCGCGGCCAGCTACCTGCCCAAGGAGCAGCTGCCGATCCTGCGGCGTGCCTGGGAAGTCGGTGCCACTGCGC
ATGCCGGGCAAACCCGCAAATCAGGCGAGCCATACATCACCCACCCGGTGGCGGTGGCCGGCGTACTGGCCGAGCTGGGC
CTGGACATGGAATCGCTGATCGCGGCGATCCTGCACGACACCATCGAAGACACTCCGCTGACGCGGGAAGAACTGGCGTT
CGAGTTCGGCGAAGCAGTGGCCGAGCTGGTAGATGGCGTCACCAAGCTGGACAAGCTCAAGTTCCGTGATCGTCAGGAAG
CGGCGGCGGAAAGCTTCCGCAAGATGCTGCTGGCGATGTCGCGCGATCTGCGCGTGATCATGATCAAGCTGGCAGACCGC
CTGCACAACATGCGCACGCTCGGCGCACAGAGCACCGAAGCGCGCGGCCGCATCGCCCGCGAGACGCTGGAAATCTACGC
ACCCATCGCGCAGCGCTTGGGCATGAGCCTGATCAAGTCTGAGCTGCAGAACCTGGGCTTCCGCGCCTTGTATCCGTGGC
GCCACGCCATCATCGAAAAACACATTCGCAGCCAGCCAGTGGTACGGCGCGAATCGATGGCGCAGGTGGAAGTGCAGCTG
TCGCAGCGGCTGGCCAAGGAAGGGTTGGAGCATCGGCTGGTCAGCCGCATCAAGACGCCCTGGAGCATCTATTCCAAGAT
GCACGAAGAGAACAAATCCTTCGACCAGGTAATGGACGTGTTCGGCTTTCGCCTGGTGGTGCGCACGGTGGCCGATTGCT
ATCACGCGCTTGGTGCGGTGCATGCCAGCTTCAAGCCGCTGGACGGGCGTTTCCGCGATTTCATCGCGATCCCCAAGGCC
AACGGCTATCAGTCGTTGCACACCGTGTTGTTCGGACCGTACGGCTCGCCGATCGAAGTGCAGATCCGCACCGAAGAGAT
GGATCTGATCGCCGAGCGTGGCGTGGCTGCGCACTGGACCTACAAGGTCGGCTCGGCCTCGCCCAATAGCGCGCAGAGTC
GCGCGCACGACTGGATCGTGGAGCTGATCGATTCGCAGCGCGCCGCTGGTTCGTCGCTGGAATTTCTCGACAACGTCAAG
GTCGACCTGTTCCCGGACGAGGTCTATCTGTTCACGCCCAAGGGCAAGATCCTGGCATTGCCGCGCAATTCCACCGCGCT
GGATTTCGCTTACGCGGTGCATACCGATGTGGGCAACCGCGCTGTGGCCTCGCGCGTGGATAAAAAGCTGGTGCCGTTGC
GCACCAAGCTGGTCAGCGGCCAGGCGGTGGAGATCATCACGGCGCGTTCGGCCACGCCCAAGCCCCAGTGGCTGGAGTTC
GTGGTCAGCAGCAAGGCGCGCACGGCGATCCGTTACCAGCTCAAGCAGCTCGAACACGAAGACGCCGTGCAGCTGGGTCA
CCGCATGCTGGACCGCGCGCTGGAAGCGATGGACTCCTCGCTGGAGCGGCTGCCCAAAGGTCGCCTGGACGCCTTTCTCA
ACGAGCACCGCTATCCGCGTCTGGAAGCGTTGCTGGCCGATGTGGCGCTCGGCAACTTGATGCCGAATCAGGCCGCGCAG
GCGTTGATGGCCTATGCGGAGATGCGTGGCGGCGGCCATTCCAAGCATTCGCACGAAAAGATTCTGATCGACGGCAGCGA
ACGCGGCGTGATCAGCTTCGCCAACTGTTGTCAGCCAATTCCCGGCGACGAAATCATGGGGTATCACACCGCCGGCAAGG
GAATCGTGGTGCACCGCCTGGATTGCCCGAACCTGGCCGAGCTGCGCAAATCGCCCGAGCGCTGGGTGCCGATCGACTGG
GATTCCAGCGTGACCGGCGACTACGACACCGCGCTGGTGGTCGAAGTGGAAAATCGCACCGGCGTGCTGGCGCAGTTGGC
CGCAGCGATCGCGCAGAGCCAGTCCAACATCGAGCGCGTGGACTATCTGGACCGCGACTTCAATGCCGCGGTGCTGCGTT
TCAACATCCAGGTGCGCGACCGCCGCCATCTGGCCGAAGTGATGCGCAGGCTGCGGCGTCTGCACGTGGTGCAGAGCGTG
GGGCGGCAGTAG

Upstream 100 bases:

>100_bases
CAGCCCGCCTAGACGCGGGCTGTTTTCGTTTCGGGCCCCTGCAGTACGCTGGCGTGTGCAAACGTTTGCCGTCACTGCAC
GGCTGGCATAGTCTTCCGCT

Downstream 100 bases:

>100_bases
CGCCTGGCACGCTCGCGCAACAGTCGAGCAAATCCGCACAAAGGCCTAAACTGCTGCAACCCTTTGCAGTGGAGTCTCCA
TGCCCCAGATCATCCATACC

Product: pentaphosphate guanosine-3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELAFEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRYQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLNEHRYPRLEALLADVALGNLMPNQAAQ
ALMAYAEMRGGGHSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSSVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Sequences:

>Translated_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELAFEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRYQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLNEHRYPRLEALLADVALGNLMPNQAAQ
ALMAYAEMRGGGHSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSSVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ
>Mature_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELAFEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRYQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLNEHRYPRLEALLADVALGNLMPNQAAQ
ALMAYAEMRGGGHSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSSVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=137, Percent_Identity=37.2262773722628, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790082, Length=695, Percent_Identity=47.9136690647482, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=687, Percent_Identity=33.1877729257642, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80918; Mature: 80918

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELAFEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRYQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLNEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNLMPNQAAQALMAYAEMRGGGHSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSSVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC
>Mature Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELAFEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRYQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLNEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNLMPNQAAQALMAYAEMRGGGHSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSSVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]