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The map label for this gene is dapF

Identifier: 84490356

GI number: 84490356

Start: 1758679

End: 1759557

Strand: Direct

Name: dapF

Synonym: Msp_1579

Alternate gene names: 84490356

Gene position: 1758679-1759557 (Clockwise)

Preceding gene: 84490355

Following gene: 84490357

Centisome position: 99.51

GC content: 30.83

Gene sequence:

>879_bases
ATGATTAAAGAAATAGAATTTACTAAAATGCATGCACTTGGTAATGATTATATTGTAATTAATGAAACAGAAACTGAAGT
TATTCCTGAAGAAGCAAAAAACAAATTAAGTAACGATATATGCACACGTAGATTTAGTGTTGGTTCTGACGGTGTAATAT
TTGCATGTAAATCAGATAAATCTGATGTTAGATTCCGTATATTTAACAGTGATGGTAGTGAAGCTGAAATGTGTGGAAAT
GGTATTCGTTGTTTAGCAAAGTACGTTTATGATAATGATATTGTTAAAAAACAAACCATGCAAATAGAAACTATGGAAGA
TATTAAAGAAGCAAGACTTACAGTTGAAGATGATGTTGTTAAAAGTATTGCTATTGATATGGGAAAGGGATTTTTCAAAC
CAGAAGAAATTCCAGCAATAGCTCCTAGTGGAAATACTGATGAATTTATTGATGAAGAAATTGATGTTGATGGTGAAAAA
ATCATAATGAGTTCTGTTAGTGTAGGAAATCCACATGCTGTTTCATTTACTGATATAAACATTGATGATATTGATTTAGA
TTATTATGGTCCACGTATTGAAAATCATAAAGCATTTCCTGAAAAAGTAAATGTTCATTTTGTAAATATTGTTTCTCCTG
GAGAAATTAACATTTTAACATGGGAACGTGGTGCAGGTTTTACATTTGCTTGTGGTACTGGTACAACAAGTTGTGTACTC
CTTGGATATAAAATGGGACTTTTAAATGAAAAAGTTCATGCACATCTCTCTGGAGGAGATTTAGATATTACAGTAAGTGA
TCATAATGAATACTTAACTGCTACAATGGAAGGAAAAGCAGTTACTGTGTATAAAGCTCAAATGACAGTGGATATTTAA

Upstream 100 bases:

>100_bases
CAGAAGTTCTTGTAAACAAAGACGAAGTAGATGTAATTAGAAGAAGAGAAGAATTCAGTGACTTATTTAATGGTCAAAAA
ATTCCTACAAGGTTATTAAA

Downstream 100 bases:

>100_bases
TTCCACCTTTTTTTTAATTTTTAGGATTGATACTATGAAGCGTATTGTTTTTGAAGATATAAGTACATTTATTGTGAAGT
ACATCTTCCATTTACGTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIKEIEFTKMHALGNDYIVINETETEVIPEEAKNKLSNDICTRRFSVGSDGVIFACKSDKSDVRFRIFNSDGSEAEMCGN
GIRCLAKYVYDNDIVKKQTMQIETMEDIKEARLTVEDDVVKSIAIDMGKGFFKPEEIPAIAPSGNTDEFIDEEIDVDGEK
IIMSSVSVGNPHAVSFTDINIDDIDLDYYGPRIENHKAFPEKVNVHFVNIVSPGEINILTWERGAGFTFACGTGTTSCVL
LGYKMGLLNEKVHAHLSGGDLDITVSDHNEYLTATMEGKAVTVYKAQMTVDI

Sequences:

>Translated_292_residues
MIKEIEFTKMHALGNDYIVINETETEVIPEEAKNKLSNDICTRRFSVGSDGVIFACKSDKSDVRFRIFNSDGSEAEMCGN
GIRCLAKYVYDNDIVKKQTMQIETMEDIKEARLTVEDDVVKSIAIDMGKGFFKPEEIPAIAPSGNTDEFIDEEIDVDGEK
IIMSSVSVGNPHAVSFTDINIDDIDLDYYGPRIENHKAFPEKVNVHFVNIVSPGEINILTWERGAGFTFACGTGTTSCVL
LGYKMGLLNEKVHAHLSGGDLDITVSDHNEYLTATMEGKAVTVYKAQMTVDI
>Mature_292_residues
MIKEIEFTKMHALGNDYIVINETETEVIPEEAKNKLSNDICTRRFSVGSDGVIFACKSDKSDVRFRIFNSDGSEAEMCGN
GIRCLAKYVYDNDIVKKQTMQIETMEDIKEARLTVEDDVVKSIAIDMGKGFFKPEEIPAIAPSGNTDEFIDEEIDVDGEK
IIMSSVSVGNPHAVSFTDINIDDIDLDYYGPRIENHKAFPEKVNVHFVNIVSPGEINILTWERGAGFTFACGTGTTSCVL
LGYKMGLLNEKVHAHLSGGDLDITVSDHNEYLTATMEGKAVTVYKAQMTVDI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=289, Percent_Identity=35.6401384083045, Blast_Score=158, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32345; Mature: 32345

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEIEFTKMHALGNDYIVINETETEVIPEEAKNKLSNDICTRRFSVGSDGVIFACKSDK
CCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCC
SDVRFRIFNSDGSEAEMCGNGIRCLAKYVYDNDIVKKQTMQIETMEDIKEARLTVEDDVV
CCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHEEEHHHHHHHHHCCEEHHHHH
KSIAIDMGKGFFKPEEIPAIAPSGNTDEFIDEEIDVDGEKIIMSSVSVGNPHAVSFTDIN
HHHHHHHCCCCCCCCCCCEECCCCCCHHHCCHHCCCCCCEEEEEEECCCCCCEEEEEECC
IDDIDLDYYGPRIENHKAFPEKVNVHFVNIVSPGEINILTWERGAGFTFACGTGTTSCVL
CCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCCEEEEECCCCCEEEE
LGYKMGLLNEKVHAHLSGGDLDITVSDHNEYLTATMEGKAVTVYKAQMTVDI
EEEHHCCCCCEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEEEEEEEEEC
>Mature Secondary Structure
MIKEIEFTKMHALGNDYIVINETETEVIPEEAKNKLSNDICTRRFSVGSDGVIFACKSDK
CCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCC
SDVRFRIFNSDGSEAEMCGNGIRCLAKYVYDNDIVKKQTMQIETMEDIKEARLTVEDDVV
CCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHEEEHHHHHHHHHCCEEHHHHH
KSIAIDMGKGFFKPEEIPAIAPSGNTDEFIDEEIDVDGEKIIMSSVSVGNPHAVSFTDIN
HHHHHHHCCCCCCCCCCCEECCCCCCHHHCCHHCCCCCCEEEEEEECCCCCCEEEEEECC
IDDIDLDYYGPRIENHKAFPEKVNVHFVNIVSPGEINILTWERGAGFTFACGTGTTSCVL
CCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCCEEEEECCCCCEEEE
LGYKMGLLNEKVHAHLSGGDLDITVSDHNEYLTATMEGKAVTVYKAQMTVDI
EEEHHCCCCCEEEEEECCCEEEEEECCCCCEEEEEECCCEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]