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The map label for this gene is gacA [H]

Identifier: 83721309

GI number: 83721309

Start: 243053

End: 243709

Strand: Reverse

Name: gacA [H]

Synonym: BTH_I0209

Alternate gene names: 83721309

Gene position: 243709-243053 (Counterclockwise)

Preceding gene: 83721311

Following gene: 83719187

Centisome position: 6.4

GC content: 64.38

Gene sequence:

>657_bases
ATGAGCCTACAGATCCTGCTCGTCGACGATCATGCGATCGTCCGCCAAGGCATCCGCCAGTTGCTGATCGACCGCGGCAT
CGCACGCGAAGTCGTGGAGGCCGAGTGCGGCGGCGAGGCGCTCGCGATCGTCGAGAAAGGCGAGTTCGACGTGGTTCTGC
TCGACATCTCGCTGCCCGACATGAACGGCATCGAAGTGCTCAAGCGCCTGAAGCGCCGGCTGCCGCGCACGCCGGTGCTG
ATGTTCTCGATGTATCGCGAGGATCAGTTCGCGGTGCGCGCGCTGAAGGCCGGCGCGGCAGGCTACCTGTCGAAGACCGT
GAACGCCGCGCAGATGGTGTCGGCGATCAGCCAGGTCGCCGCGGGCCGCAAGTACGTGAGCCCGGCGATGGCCGAGGCGC
TCGCCGAGTACGTGTCGTTCGAGAACGAGCCGCTGCCGCACGAGAAGCTGTCGGACCGCGAATACCAGACGCTCTGCATG
ATCGCGTCGGGCAAGCGGCTCACGGACATCGCGCACGCGCTGTCGCTATCGGTGAAGACGGTCAGCGTCTATCGGACGCG
CCTGCTCGAGAAAATGAAGCTGTCGAACAACGCGGAACTCACGTTCTATGTGATGAGCAACCGCCTCGTCGACATGAATC
CGGCGATCGGCGCGTAA

Upstream 100 bases:

>100_bases
CGGGCGGCGCGGGCACCGCGCTGCGCGCGCGCTTCGCCTGGGGCGCGCTCGTCGCCGCACGCACGCCGGCCGCCGCGCGC
AGGGTGCGCAAGGAGGCGCG

Downstream 100 bases:

>100_bases
TGCGGCGCGGCGGCAGCCCGGCCTCGGCCGCCTGAATACGAAGCGTGCGCGCCCTGCGGCGCGGCGCCGCAGGCGGACAA
TTGATGCAAGCAATTGTTTT

Product: LuxR family DNA-binding response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVVEAECGGEALAIVEKGEFDVVLLDISLPDMNGIEVLKRLKRRLPRTPVL
MFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
IASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA

Sequences:

>Translated_218_residues
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVVEAECGGEALAIVEKGEFDVVLLDISLPDMNGIEVLKRLKRRLPRTPVL
MFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
IASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
>Mature_217_residues
SLQILLVDDHAIVRQGIRQLLIDRGIAREVVEAECGGEALAIVEKGEFDVVLLDISLPDMNGIEVLKRLKRRLPRTPVLM
FSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMI
ASGKRLTDIAHALSLSVKTVSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=36.0189573459716, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=36.8686868686869, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=33.0143540669856, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=29.1866028708134, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=32.8502415458937, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786747, Length=212, Percent_Identity=27.8301886792453, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=33.6134453781513, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788546, Length=217, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=107, Percent_Identity=40.1869158878505, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24142; Mature: 24010

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQILLVDDHAIVRQGIRQLLIDRGIAREVVEAECGGEALAIVEKGEFDVVLLDISLPD
CCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCC
MNGIEVLKRLKRRLPRTPVLMFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVA
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMIASGKRLTDIAHALSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHH
VSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
HHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCC
>Mature Secondary Structure 
SLQILLVDDHAIVRQGIRQLLIDRGIAREVVEAECGGEALAIVEKGEFDVVLLDISLPD
CEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCC
MNGIEVLKRLKRRLPRTPVLMFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQVA
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMIASGKRLTDIAHALSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHEEEEEECCCHHHHHHHHHHHHHHH
VSVYRTRLLEKMKLSNNAELTFYVMSNRLVDMNPAIGA
HHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]