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The map label for this gene is gloB [H]

Identifier: 83720134

GI number: 83720134

Start: 3204365

End: 3205171

Strand: Reverse

Name: gloB [H]

Synonym: BTH_I2788

Alternate gene names: 83720134

Gene position: 3205171-3204365 (Counterclockwise)

Preceding gene: 161723131

Following gene: 83719197

Centisome position: 84.14

GC content: 71.75

Gene sequence:

>807_bases
ATGAACGAGCTGGAATACGTGCCGGTGCCGGCATTCGACGACAACTACATCTGGCTCGTATCGGACGGCCGCCATGCGGT
CGCCGTCGATCCGGGCGAAGCCGCGCCGGTGCGTCGCCATCTCGACGCGCGAGGCTGGCGACTGACCGCTATTTTACTCA
CGCACCACCACGGCGACCATGTCGGCGGAGTGGCCGAGCTGCTCGCGACCGCGCGCGAAGGCGAGCCGCTCGCGGTGTTC
GGCCCCGCGCACGAGGCGATCGAGCACCTCACGCACCGCGTGACGGGCGGCGAGCGCGTGCGCATCGGTTCGCCCGCGTT
CGAGTTCGACGTGATCGACGTGCCGGGCCACACGCGCGGCCACATCGCGTACTTCCAGCCGGCCGGGCCGGGCAACGCCG
CGCCGCACCTCTTTTGCGGCGATACGCTGTTCTCGTGCGGCTGCGGGCGCCTCTTCGAGGGCACGCCCGCGCAGATGCTC
GCGTCGCTCGACGCGCTCGCGGCGCTGCCGGGCGACACGCGCGTGCACTGCGCGCACGAGTACACGCTGTCGAACATCCG
GTTCGCGCTCGCGTGCGAGCCGGACAACGCGGCGCTCGCCGCGTGGCGCGACGAAGCGCTCGCGCGCCGCGCGCGCCACG
AGCCGACGCTGCCGACGACGATCGCGCACGAGCGCGCGGTGAACCCGTTCCTGCGCGCGGACAGCCCGTCGATCCGCGCG
ACGCTCGAAGCGCAACTGCACGAGGGGGCGCCCGACCGGCTCGCGGCGTTCACGCTGATGCGCGAGTGGAAAAACCGTTT
TCGTTGA

Upstream 100 bases:

>100_bases
GCCTGCTCCCAGCCGAGCACGTAGCGTCCGGGCGGGGAGTCGGTCCAGGCGGGCCAGTCTATAATCGAACGGTCAGACAT
ATCTCGGTGATTGCGCGCCC

Downstream 100 bases:

>100_bases
GCGCGCGAGAGCCGGGGCAAGCTCAACCGAAATCTGGAGAATCGGCCCCAAATACCCGATTTAGCAGGTTTTTTCGGCCT
TTTTGTTGACGCGAAGCACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNELEYVPVPAFDDNYIWLVSDGRHAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHGDHVGGVAELLATAREGEPLAVF
GPAHEAIEHLTHRVTGGERVRIGSPAFEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPDNAALAAWRDEALARRARHEPTLPTTIAHERAVNPFLRADSPSIRA
TLEAQLHEGAPDRLAAFTLMREWKNRFR

Sequences:

>Translated_268_residues
MNELEYVPVPAFDDNYIWLVSDGRHAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHGDHVGGVAELLATAREGEPLAVF
GPAHEAIEHLTHRVTGGERVRIGSPAFEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPDNAALAAWRDEALARRARHEPTLPTTIAHERAVNPFLRADSPSIRA
TLEAQLHEGAPDRLAAFTLMREWKNRFR
>Mature_268_residues
MNELEYVPVPAFDDNYIWLVSDGRHAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHGDHVGGVAELLATAREGEPLAVF
GPAHEAIEHLTHRVTGGERVRIGSPAFEFDVIDVPGHTRGHIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTRVHCAHEYTLSNIRFALACEPDNAALAAWRDEALARRARHEPTLPTTIAHERAVNPFLRADSPSIRA
TLEAQLHEGAPDRLAAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=38.7832699619772, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI94538322, Length=266, Percent_Identity=39.0977443609023, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=42.4892703862661, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI116642887, Length=244, Percent_Identity=36.0655737704918, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI21703352, Length=244, Percent_Identity=36.0655737704918, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI46361987, Length=210, Percent_Identity=41.9047619047619, Blast_Score=128, Evalue=4e-30,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=45.8015267175573, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=34.1968911917098, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=260, Percent_Identity=33.4615384615385, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=31.5985130111524, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=271, Percent_Identity=29.1512915129151, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356335, Length=269, Percent_Identity=37.9182156133829, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24667711, Length=269, Percent_Identity=37.9182156133829, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24667703, Length=269, Percent_Identity=37.9182156133829, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29306; Mature: 29306

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRHAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHGDH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHCCCCEEEEEEEEEECCCCC
VGGVAELLATAREGEPLAVFGPAHEAIEHLTHRVTGGERVRIGSPAFEFDVIDVPGHTRG
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPDNAALAAWRDEALARRARHEPTLPTTIAHERAVNPFLRADSPSIRA
EEECCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCEE
TLEAQLHEGAPDRLAAFTLMREWKNRFR
EEEEHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRHAVAVDPGEAAPVRRHLDARGWRLTAILLTHHHGDH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHCCCCEEEEEEEEEECCCCC
VGGVAELLATAREGEPLAVFGPAHEAIEHLTHRVTGGERVRIGSPAFEFDVIDVPGHTRG
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCC
HIAYFQPAGPGNAAPHLFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTRVHCAHE
CEEEECCCCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPDNAALAAWRDEALARRARHEPTLPTTIAHERAVNPFLRADSPSIRA
EEECCEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCEE
TLEAQLHEGAPDRLAAFTLMREWKNRFR
EEEEHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA