Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 83719197

GI number: 83719197

Start: 3202618

End: 3204213

Strand: Reverse

Name: mltD [H]

Synonym: BTH_I2787

Alternate gene names: 83719197

Gene position: 3204213-3202618 (Counterclockwise)

Preceding gene: 83720134

Following gene: 83721301

Centisome position: 84.12

GC content: 67.73

Gene sequence:

>1596_bases
ATGCGATTCCTTCTTAGTGCGCTGTTGGTCCTGACGCTCGCCGCCTGCGCGGGCACCGGGCCGACCGCCCAAAATTCCGC
GGCCTCCGACCCGAACGCCGCTTCGGACTATTTGCGCAAGTCCGCCTCCGCGAAAGAAACCGTCGACGTCGACAAGCAAT
CGGTCGGCGACCTCACCACCGCCGACAGCGATCTCTGGGCGCGCATTCGCCGCGGCTTCCAGATGCCCGATCTGCGGAGC
GACCTCGTCGACATGCAGGCGTCGTGGTACGCGCAGCGTCCCGACTACGTGCAGCGGATGACCGAGCGTTCGCAGAAGTA
CCTGTATCACATCGTCGAGGAACTCGAGTCGCGCCACATGCCGACCGAGCTCGCGCTGCTGCCGTTCATCGAATCGGCGT
ACAACCCGCAGGCGCTGTCCGTCGCGAAGGCGGCGGGGATGTGGCAGTTCATGCCGGCCACGGGGCGCACGTTCAACCTG
AAGCGCAACATGTGGCAGGACGAGCGGCGCGACGTGCTCGCGTCGACGAGCGCCGCGCTCGATTACTTGTCGCGCCTGCA
TGACATGTTCGGCGACTGGTATCTCGCGCTCGCCGCGTACAACTGGGGCGAGGGCAACGTGCAGCGCGCGATCGCGCGCA
ACCAGGCGGCCGGCCTGCCGACCGACTACCAGAGCCTGCGGATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAG
GCAGTCAAGAACATCGTGATGAACCCGCAGCAGTATGGCCTGACGCTGCCGGAGATTCCGAACCACCCGTATTTCGTGAC
GGTCACGACGTCGCGCGACATCGACGTGAAGGTCGCCGCGAAGCTCGCGAACCTGCCGCTCGACGAATTCAAGTCGCTCA
ACCCGTCGTTCTCGAAGCCGGTGATCCTCGGCGCGACGCAGCCGCAGATTCTGCTGCCGTTCGACAACGCGTCGGCGTTC
GAGAAGGGGCTCAAGACGTACGACGGCCAACTGTCGTCGTGGACCGCGTATACGGTCACCGAGCGCGCGCGGCCCGCCGC
GATCGCGGAGAAGATCGGCGTCGACGCGGACACGCTGATGCAGGTGAACAAGATTCCGGCGGGCATGCGGCTGAAGCCCG
GTTCGACGATCGTCGTGCCGCGCACCGACGACGACGACGAGGACATCAGCGCCGACGTCGCCGAGAACGGCGTGCTCGCG
ATGGAGCCCGACGTGCCGGATACGCGCAAGATGCTGATCCGCGTGCGCCGCAAGCAGTCGATGGAGGCGCTCGCGAACCG
CTACGGCGTATCGGTCGCGCAGCTGCGTGGATGGAACCGCACGCGTCGCGCGGTCGTGATGCCCGGCCAGACGATGGTGC
TGCACGTGCCCGTCGGCCGCGCGGTGCCGGCGGAGCCCGGGCCCGAGCGGATCGCGACGTCGGTTGGAGGCGGTCGCATC
GAGCGCGCGAGCCTGCGCACGGGCGGCAAGGCGTCGAGCCGGGGCCGCAAGGCGGCGCCCGTCGCGTCGAAGGCGTCGCC
GAAGGCCGCGGCGAAGAGCGGCGCGAAGCCCGCGCACGCGAAGACCGCGAGCCGCAAGACGACGAAGAAAAAGTAG

Upstream 100 bases:

>100_bases
ATACCCGATTTAGCAGGTTTTTTCGGCCTTTTTGTTGACGCGAAGCACGCACTTTCGTACTATCGCCTGCAATTTCCAGT
TGTTGGAAGCCGAGATTTTC

Downstream 100 bases:

>100_bases
GACGAAAGGGCGCGCACGCCGCGGGGGCTTTCCGGCCGTCGGCGCGCAATCGACTATCATCGCCGGACGCCGTCACCTGG
GTGACGGCGTTTTCGTTTGT

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MRFLLSALLVLTLAACAGTGPTAQNSAASDPNAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLRS
DLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNL
KRNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQ
AVKNIVMNPQQYGLTLPEIPNHPYFVTVTTSRDIDVKVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNASAF
EKGLKTYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLA
MEPDVPDTRKMLIRVRRKQSMEALANRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRI
ERASLRTGGKASSRGRKAAPVASKASPKAAAKSGAKPAHAKTASRKTTKKK

Sequences:

>Translated_531_residues
MRFLLSALLVLTLAACAGTGPTAQNSAASDPNAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLRS
DLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNL
KRNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQ
AVKNIVMNPQQYGLTLPEIPNHPYFVTVTTSRDIDVKVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNASAF
EKGLKTYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLA
MEPDVPDTRKMLIRVRRKQSMEALANRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRI
ERASLRTGGKASSRGRKAAPVASKASPKAAAKSGAKPAHAKTASRKTTKKK
>Mature_531_residues
MRFLLSALLVLTLAACAGTGPTAQNSAASDPNAASDYLRKSASAKETVDVDKQSVGDLTTADSDLWARIRRGFQMPDLRS
DLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNL
KRNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQ
AVKNIVMNPQQYGLTLPEIPNHPYFVTVTTSRDIDVKVAAKLANLPLDEFKSLNPSFSKPVILGATQPQILLPFDNASAF
EKGLKTYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLMQVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLA
MEPDVPDTRKMLIRVRRKQSMEALANRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRI
ERASLRTGGKASSRGRKAAPVASKASPKAAAKSGAKPAHAKTASRKTTKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=469, Percent_Identity=32.6226012793177, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58300; Mature: 58300

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLLSALLVLTLAACAGTGPTAQNSAASDPNAASDYLRKSASAKETVDVDKQSVGDLTT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCHHHCCCCCC
ADSDLWARIRRGFQMPDLRSDLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDERRDVLASTSAAL
CHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCEEECHHHCCHHHHHHHHHHHHHHH
DYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQ
HHHHHHHHHHHHHEEHHHEECCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHH
AVKNIVMNPQQYGLTLPEIPNHPYFVTVTTSRDIDVKVAAKLANLPLDEFKSLNPSFSKP
HHHHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCCCCCC
VILGATQPQILLPFDNASAFEKGLKTYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLM
EEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHH
QVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQS
HHHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
MEALANRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRI
HHHHHHHHCCCHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
ERASLRTGGKASSRGRKAAPVASKASPKAAAKSGAKPAHAKTASRKTTKKK
EEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MRFLLSALLVLTLAACAGTGPTAQNSAASDPNAASDYLRKSASAKETVDVDKQSVGDLTT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCHHHCCCCCC
ADSDLWARIRRGFQMPDLRSDLVDMQASWYAQRPDYVQRMTERSQKYLYHIVEELESRHM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PTELALLPFIESAYNPQALSVAKAAGMWQFMPATGRTFNLKRNMWQDERRDVLASTSAAL
CHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCEEECHHHCCHHHHHHHHHHHHHHH
DYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQ
HHHHHHHHHHHHHEEHHHEECCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHH
AVKNIVMNPQQYGLTLPEIPNHPYFVTVTTSRDIDVKVAAKLANLPLDEFKSLNPSFSKP
HHHHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCCCCCC
VILGATQPQILLPFDNASAFEKGLKTYDGQLSSWTAYTVTERARPAAIAEKIGVDADTLM
EEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHH
QVNKIPAGMRLKPGSTIVVPRTDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQS
HHHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
MEALANRYGVSVAQLRGWNRTRRAVVMPGQTMVLHVPVGRAVPAEPGPERIATSVGGGRI
HHHHHHHHCCCHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
ERASLRTGGKASSRGRKAAPVASKASPKAAAKSGAKPAHAKTASRKTTKKK
EEHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]