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The map label for this gene is lpxD [H]

Identifier: 83718496

GI number: 83718496

Start: 2309282

End: 2310367

Strand: Direct

Name: lpxD [H]

Synonym: BTH_I2037

Alternate gene names: 83718496

Gene position: 2309282-2310367 (Clockwise)

Preceding gene: 83719406

Following gene: 83721525

Centisome position: 60.62

GC content: 69.52

Gene sequence:

>1086_bases
ATGGCATTGACGCTTGAGGCGCTCGTCGCGCGGTTCGGCGGCGAGATCGTCGGCGACGGCCGGCGCGAGGTCGGCGGTCT
CGCGCCGCTCGATCAGGCTGGCCCGCAGCACCTCGCGTTCCTCGCGAATCCGAAGTATCTGGCGCAGGTCGAGACGACCG
CCGCCGGCGCGGTGCTGATCGCGCCGCGTGATCTCGAGAAGCTCGGCGCGGCCGCGAACGGCCGCAATTTCATCATCACG
CCGAATCCCTACGCGTATTTCGCGCGGGTCGCGCAGATGTTCATCGATCTCGCCGCGCCGCAGCGCGCGGCCGGCGTGCA
TCCAAGCGCGACGATCGATCCTGCCGCGCAGGTCGCCGCGAGCGCGGTGATCGGCCCGCACGTGAGCGTCGAGGCGGGCG
CCGTGATCGGCGAGCGCGTGCAACTCGACGCGAACGTGTTCGTCGGGCGCGGCACGCGGATCGGCGACGACTCGCACCTG
TACCCGAACGTCACGATCTATCACGGCTGCACGCTCGGGCCGCGCGCGATCGTCCATTCGGGCGCCGTGATCGGCTCCGA
CGGCTTCGGCTTCGCGCCGGATTTCGTCGGCGAAGGCGACGCGCGCACGGGCGCGTGGGTCAAGATTCCGCAGGTCGGCG
GCGTGAAGGTCGGCCCCGACGTCGAGATCGGCGCGAACACGACGATCGACCGCGGCGCGATGGCGGATACCGTCATCGAC
GAATGCGTGAAGATCGACAATCTCGTGCAGATCGGCCACAACTGCCGGATCGGCGCATACACGGTGATCGCCGGCTGCGC
GGGCATCGCGGGCAGCACGACGATCGGCAAGCACTGCATGATCGGCGGCGCGGTCGGCGTCGCCGGCCACGTGACGCTCG
GCGACTACGTGATCGTCACCGCGAAGTCGGGCGTATCGAAATCGCTGCCGAAGGCCGGCATCTATACGAGCGCGTTTCCG
GCCGTCGAGCACGGCGACTGGAACAAGAGCGCGGCGCTCGTGCGCAATCTGGACAAGCTGCGCGATCGGATCAAGGCGCT
CGAGACCGCACTGGCCGCGCGGGAAGGCGACGCCGGCGGCGCATGA

Upstream 100 bases:

>100_bases
GAAGCGGTGTACGTGAGTCCGCGCATCGACATCACCGACAAGGTGCTGAAGGCGCTCGCATCGCCTTCGAGCCTGTCGAA
CTGAACGGAGCGAACGTCGA

Downstream 100 bases:

>100_bases
ACTCACGTCGGCCGGATCTCGCGACGCAGCACTCGCAGGCTGCCCGCCGCGCCGGCCGATCGAACCGCGCGCCGCTTTCC
CCAACGCGGCCCGCATCAGC

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MALTLEALVARFGGEIVGDGRREVGGLAPLDQAGPQHLAFLANPKYLAQVETTAAGAVLIAPRDLEKLGAAANGRNFIIT
PNPYAYFARVAQMFIDLAAPQRAAGVHPSATIDPAAQVAASAVIGPHVSVEAGAVIGERVQLDANVFVGRGTRIGDDSHL
YPNVTIYHGCTLGPRAIVHSGAVIGSDGFGFAPDFVGEGDARTGAWVKIPQVGGVKVGPDVEIGANTTIDRGAMADTVID
ECVKIDNLVQIGHNCRIGAYTVIAGCAGIAGSTTIGKHCMIGGAVGVAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFP
AVEHGDWNKSAALVRNLDKLRDRIKALETALAAREGDAGGA

Sequences:

>Translated_361_residues
MALTLEALVARFGGEIVGDGRREVGGLAPLDQAGPQHLAFLANPKYLAQVETTAAGAVLIAPRDLEKLGAAANGRNFIIT
PNPYAYFARVAQMFIDLAAPQRAAGVHPSATIDPAAQVAASAVIGPHVSVEAGAVIGERVQLDANVFVGRGTRIGDDSHL
YPNVTIYHGCTLGPRAIVHSGAVIGSDGFGFAPDFVGEGDARTGAWVKIPQVGGVKVGPDVEIGANTTIDRGAMADTVID
ECVKIDNLVQIGHNCRIGAYTVIAGCAGIAGSTTIGKHCMIGGAVGVAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFP
AVEHGDWNKSAALVRNLDKLRDRIKALETALAAREGDAGGA
>Mature_360_residues
ALTLEALVARFGGEIVGDGRREVGGLAPLDQAGPQHLAFLANPKYLAQVETTAAGAVLIAPRDLEKLGAAANGRNFIITP
NPYAYFARVAQMFIDLAAPQRAAGVHPSATIDPAAQVAASAVIGPHVSVEAGAVIGERVQLDANVFVGRGTRIGDDSHLY
PNVTIYHGCTLGPRAIVHSGAVIGSDGFGFAPDFVGEGDARTGAWVKIPQVGGVKVGPDVEIGANTTIDRGAMADTVIDE
CVKIDNLVQIGHNCRIGAYTVIAGCAGIAGSTTIGKHCMIGGAVGVAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFPA
VEHGDWNKSAALVRNLDKLRDRIKALETALAAREGDAGGA

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=354, Percent_Identity=39.8305084745763, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36953; Mature: 36822

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLEALVARFGGEIVGDGRREVGGLAPLDQAGPQHLAFLANPKYLAQVETTAAGAVLI
CEEEHHHHHHHHCCCEECCCHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEEE
APRDLEKLGAAANGRNFIITPNPYAYFARVAQMFIDLAAPQRAAGVHPSATIDPAAQVAA
CCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHH
SAVIGPHVSVEAGAVIGERVQLDANVFVGRGTRIGDDSHLYPNVTIYHGCTLGPRAIVHS
HHHCCCCCEEECCCEECCEEEEECEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHCC
GAVIGSDGFGFAPDFVGEGDARTGAWVKIPQVGGVKVGPDVEIGANTTIDRGAMADTVID
CCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEECCCCCCCCCHHHHHHHH
ECVKIDNLVQIGHNCRIGAYTVIAGCAGIAGSTTIGKHCMIGGAVGVAGHVTLGDYVIVT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCEEEEE
AKSGVSKSLPKAGIYTSAFPAVEHGDWNKSAALVRNLDKLRDRIKALETALAAREGDAGG
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
A
C
>Mature Secondary Structure 
ALTLEALVARFGGEIVGDGRREVGGLAPLDQAGPQHLAFLANPKYLAQVETTAAGAVLI
EEEHHHHHHHHCCCEECCCHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEEE
APRDLEKLGAAANGRNFIITPNPYAYFARVAQMFIDLAAPQRAAGVHPSATIDPAAQVAA
CCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHH
SAVIGPHVSVEAGAVIGERVQLDANVFVGRGTRIGDDSHLYPNVTIYHGCTLGPRAIVHS
HHHCCCCCEEECCCEECCEEEEECEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHCC
GAVIGSDGFGFAPDFVGEGDARTGAWVKIPQVGGVKVGPDVEIGANTTIDRGAMADTVID
CCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEECCCCCCCCCHHHHHHHH
ECVKIDNLVQIGHNCRIGAYTVIAGCAGIAGSTTIGKHCMIGGAVGVAGHVTLGDYVIVT
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCEEEEE
AKSGVSKSLPKAGIYTSAFPAVEHGDWNKSAALVRNLDKLRDRIKALETALAAREGDAGG
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA