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The map label for this gene is def [H]

Identifier: 284159413

GI number: 284159413

Start: 157957

End: 158496

Strand: Direct

Name: def [H]

Synonym: BTH_I0128

Alternate gene names: 284159413

Gene position: 157957-158496 (Clockwise)

Preceding gene: 83719370

Following gene: 83718636

Centisome position: 4.15

GC content: 61.85

Gene sequence:

>540_bases
ATGCGCGCGGGTCGTTCGTCTCTCAACACTGAAATCATGGCTTTGCTGAACATCCTTCATTACCCCGACAAACGGCTGCA
CAAGGTGGCCAAGCCGGTCGACAAGGTGGACGACCGTATTCGCAGGCTCGTCGCCGACATGGCCGAGACGATGTACGCGG
CGCCCGGCATCGGGCTCGCGGCGACGCAGGTCGACGTGCACGAGCGCGTGATCGTGATCGACGTGTCCGAGGACAAGAAC
GAGCTGCGCGTTTTCATCAATCCGGAGATCGTCTGGACGAGCGACGGCAAGCAGGTCTACGAGGAAGGCTGTCTGTCGGT
GCCGGGCGTCTATGACGAAGTCGAGCGGCCTGACCGCGTGCGCGTGCGCGCGCTCGACGAGCAAGGCGAGCCGTTCGAGC
TCGATTGCGAAGGGCTGCTCGCGGTGTGCATCCAGCACGAGATGGATCACCTGATGGGCCGCGTGTTCGTCCAGTATCTG
TCGCCGCTCAAGCAGACCCGGATCAAGACGAAGATGAAGAAACTCGAACGCGCAATGTGA

Upstream 100 bases:

>100_bases
ACGGCGATTGGCCGGTCATTGCGACGCGCGGCGGATTCCGCGCGTCGTCGAATCCATCTTGAGGCCGGCGCGCGCGTCGC
GGGAGTCGGCCGAACGGCCG

Downstream 100 bases:

>100_bases
TGCGCATGGCTGCCCCTAGATTCATTCGTACATGACACATTCACTGCGCGTCATTTTTGCCGGCACGCCGGAATTCGCCG
CGGCGGCGCTCGCCGCGATT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MRAGRSSLNTEIMALLNILHYPDKRLHKVAKPVDKVDDRIRRLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTSDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDEQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM

Sequences:

>Translated_179_residues
MRAGRSSLNTEIMALLNILHYPDKRLHKVAKPVDKVDDRIRRLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTSDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDEQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM
>Mature_179_residues
MRAGRSSLNTEIMALLNILHYPDKRLHKVAKPVDKVDDRIRRLVADMAETMYAAPGIGLAATQVDVHERVIVIDVSEDKN
ELRVFINPEIVWTSDGKQVYEEGCLSVPGVYDEVERPDRVRVRALDEQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYL
SPLKQTRIKTKMKKLERAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=154, Percent_Identity=30.5194805194805, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=60, Blast_Score=206, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24645728, Length=143, Percent_Identity=35.6643356643357, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=31.3868613138686, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20472; Mature: 20472

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGRSSLNTEIMALLNILHYPDKRLHKVAKPVDKVDDRIRRLVADMAETMYAAPGIGLA
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ATQVDVHERVIVIDVSEDKNELRVFINPEIVWTSDGKQVYEEGCLSVPGVYDEVERPDRV
EEEEHHHCEEEEEEECCCCCCEEEEECCEEEEECCCHHHHHHCCCCCCCCHHHHCCCCCE
RVRALDEQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYLSPLKQTRIKTKMKKLERAM
EEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRAGRSSLNTEIMALLNILHYPDKRLHKVAKPVDKVDDRIRRLVADMAETMYAAPGIGLA
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ATQVDVHERVIVIDVSEDKNELRVFINPEIVWTSDGKQVYEEGCLSVPGVYDEVERPDRV
EEEEHHHCEEEEEEECCCCCCEEEEECCEEEEECCCHHHHHHCCCCCCCCHHHHCCCCCE
RVRALDEQGEPFELDCEGLLAVCIQHEMDHLMGRVFVQYLSPLKQTRIKTKMKKLERAM
EEEEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]