Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 83718636

GI number: 83718636

Start: 158528

End: 159514

Strand: Direct

Name: fmt

Synonym: BTH_I0129

Alternate gene names: 83718636

Gene position: 158528-159514 (Clockwise)

Preceding gene: 284159413

Following gene: 83719269

Centisome position: 4.16

GC content: 72.04

Gene sequence:

>987_bases
ATGACACATTCACTGCGCGTCATTTTTGCCGGCACGCCGGAATTCGCCGCGGCGGCGCTCGCCGCGATTCATGAGGCCGG
TTTCCCGGTGCCGCTCGTGCTGACGCAGCCCGATCGTCCCGCCGGGCGCGGGATGAAGCTGCAGGCGAGCGCCGTGAAGC
GCTATGCGCTCGAGCACGGCATCACGGTCGCGCAACCGCCTTCGCTGCGACGCGCGGGAAAGCACCCGGCCGAAGCCGCC
GCCGCGCTCGATTTGCTGCACGCGACGCCGCACGACGTGATGGTCGTCGCCGCATATGGCCTGCTTCTGCCGCAAGAAGT
GCTCGACCTGCCGCGATACGGCTGCATCAATATCCACGCGTCGCTGCTGCCGCGCTGGCGCGGCGCCGCGCCGATCCATC
GCGCGATCGAGGCGGGCGATGCCGAGACGGGCGTCACGCTGATGCAGATGGACGCCGGGCTCGACACGGGCGCGATGCTG
CACGACGCGCGCGTCGCGATCGCGCCCGACGATACGACGGCGACGCTGCACGACAAGCTCGCGGCGGCCGGCGCGACTCT
CATCGTCGACGCGCTCGTCGAGCTCGAGCGAGCGGGCGCGCTTGCCGCGACGCCGCAGCCCGACGACGGCATCACCTATG
CCGAGAAGATCGGCAAGCACGAGGCGGCACTCGACTGGCGCAAGCCGGCCACCGTGCTCGCGCGGCAGGTGCGCGCGTTC
GACCCGTTTCCGGGCGGCGCGGGCACGCTCGACGGCGTGACGCTCAAGCTGTGGGCGGCCGACGCGATGCCGGCGCGCGG
CGACGCAGCGCCCGGCACGATCGTCGATGCCGGCGCGGACGGCGTCGTGATCGCCTGCGGCGAAGGTGCGCTGCGCGTCA
CGCAATTGCAGAAGCCGGGCGGCAAGCGTCTGCCGGCGCGCGAGTTTCTCGCGGGGGCGCCGCTTGCGGTCGGCCAACGT
TTCTCGCCGCCCGACACCGCCGCGTAA

Upstream 100 bases:

>100_bases
CAGTATCTGTCGCCGCTCAAGCAGACCCGGATCAAGACGAAGATGAAGAAACTCGAACGCGCAATGTGATGCGCATGGCT
GCCCCTAGATTCATTCGTAC

Downstream 100 bases:

>100_bases
TTGCGTCGTCTGCCTACAGTTCATCGAAATCGCGCGCGCCGCGCAATCGGCCGAACGGCCAGGTCGCGGCGCGCAGGGCG
CGCGCGTAGAATTCTCTGAC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTHSLRVIFAGTPEFAAAALAAIHEAGFPVPLVLTQPDRPAGRGMKLQASAVKRYALEHGITVAQPPSLRRAGKHPAEAA
AALDLLHATPHDVMVVAAYGLLLPQEVLDLPRYGCINIHASLLPRWRGAAPIHRAIEAGDAETGVTLMQMDAGLDTGAML
HDARVAIAPDDTTATLHDKLAAAGATLIVDALVELERAGALAATPQPDDGITYAEKIGKHEAALDWRKPATVLARQVRAF
DPFPGGAGTLDGVTLKLWAADAMPARGDAAPGTIVDAGADGVVIACGEGALRVTQLQKPGGKRLPAREFLAGAPLAVGQR
FSPPDTAA

Sequences:

>Translated_328_residues
MTHSLRVIFAGTPEFAAAALAAIHEAGFPVPLVLTQPDRPAGRGMKLQASAVKRYALEHGITVAQPPSLRRAGKHPAEAA
AALDLLHATPHDVMVVAAYGLLLPQEVLDLPRYGCINIHASLLPRWRGAAPIHRAIEAGDAETGVTLMQMDAGLDTGAML
HDARVAIAPDDTTATLHDKLAAAGATLIVDALVELERAGALAATPQPDDGITYAEKIGKHEAALDWRKPATVLARQVRAF
DPFPGGAGTLDGVTLKLWAADAMPARGDAAPGTIVDAGADGVVIACGEGALRVTQLQKPGGKRLPAREFLAGAPLAVGQR
FSPPDTAA
>Mature_327_residues
THSLRVIFAGTPEFAAAALAAIHEAGFPVPLVLTQPDRPAGRGMKLQASAVKRYALEHGITVAQPPSLRRAGKHPAEAAA
ALDLLHATPHDVMVVAAYGLLLPQEVLDLPRYGCINIHASLLPRWRGAAPIHRAIEAGDAETGVTLMQMDAGLDTGAMLH
DARVAIAPDDTTATLHDKLAAAGATLIVDALVELERAGALAATPQPDDGITYAEKIGKHEAALDWRKPATVLARQVRAFD
PFPGGAGTLDGVTLKLWAADAMPARGDAAPGTIVDAGADGVVIACGEGALRVTQLQKPGGKRLPAREFLAGAPLAVGQRF
SPPDTAA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=329, Percent_Identity=27.355623100304, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21614513, Length=325, Percent_Identity=28, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI164663775, Length=232, Percent_Identity=32.3275862068966, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=49.3548387096774, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788589, Length=317, Percent_Identity=31.2302839116719, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI133930964, Length=326, Percent_Identity=26.3803680981595, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=29.8412698412698, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=34.070796460177, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585660, Length=252, Percent_Identity=25.3968253968254, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_BURTA (Q2T2B1)

Other databases:

- EMBL:   CP000086
- RefSeq:   YP_440688.1
- HSSP:   P77398
- ProteinModelPortal:   Q2T2B1
- SMR:   Q2T2B1
- STRING:   Q2T2B1
- GeneID:   3847172
- GenomeReviews:   CP000086_GR
- KEGG:   bte:BTH_I0129
- TIGR:   BTH_I0129
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   BTHA271848:BTH_I0129-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33973; Mature: 33842

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSLRVIFAGTPEFAAAALAAIHEAGFPVPLVLTQPDRPAGRGMKLQASAVKRYALEHG
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHC
ITVAQPPSLRRAGKHPAEAAAALDLLHATPHDVMVVAAYGLLLPQEVLDLPRYGCINIHA
CEECCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCEEEEHH
SLLPRWRGAAPIHRAIEAGDAETGVTLMQMDAGLDTGAMLHDARVAIAPDDTTATLHDKL
HHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCEEEECCCCCCHHHHHHH
AAAGATLIVDALVELERAGALAATPQPDDGITYAEKIGKHEAALDWRKPATVLARQVRAF
HHCCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHC
DPFPGGAGTLDGVTLKLWAADAMPARGDAAPGTIVDAGADGVVIACGEGALRVTQLQKPG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEECCCCEEEEEECCCC
GKRLPAREFLAGAPLAVGQRFSPPDTAA
CCCCCHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
THSLRVIFAGTPEFAAAALAAIHEAGFPVPLVLTQPDRPAGRGMKLQASAVKRYALEHG
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHC
ITVAQPPSLRRAGKHPAEAAAALDLLHATPHDVMVVAAYGLLLPQEVLDLPRYGCINIHA
CEECCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCEEEEHH
SLLPRWRGAAPIHRAIEAGDAETGVTLMQMDAGLDTGAMLHDARVAIAPDDTTATLHDKL
HHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCEEEECCCCCCHHHHHHH
AAAGATLIVDALVELERAGALAATPQPDDGITYAEKIGKHEAALDWRKPATVLARQVRAF
HHCCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHC
DPFPGGAGTLDGVTLKLWAADAMPARGDAAPGTIVDAGADGVVIACGEGALRVTQLQKPG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEECCCCEEEEEECCCC
GKRLPAREFLAGAPLAVGQRFSPPDTAA
CCCCCHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA