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The map label for this gene is trmE [H]

Identifier: 161723129

GI number: 161723129

Start: 3684950

End: 3686353

Strand: Reverse

Name: trmE [H]

Synonym: BTH_I3233

Alternate gene names: 161723129

Gene position: 3686353-3684950 (Counterclockwise)

Preceding gene: 83719666

Following gene: 83720961

Centisome position: 96.77

GC content: 68.38

Gene sequence:

>1404_bases
ATGCTCGCCACTGATTCCGATCCGATCGTCGCCATTGCCACCGCGTCCGGCCGAGGCGGAATCGGCGTTGTGCGCATTTC
GCTTGGGCGAGCCGGGGAGGCGGCGGCGCTTGCGCTGAGCGACGCGCTGTGCGGTGCGCGGCTCACGCCGAGGCATGCGA
GCTATGTGCCGTTTCTCGATGGCGCAGGCGAGCCGCTCGACCGGGGCATCGCGCTCTATTTTCCGGCGCCGCATTCGTAC
ACGGGGGAGCACGTGCTCGAGCTGCAAGGTCACGGCGGGCCGATCGTGCTGCAGCTCGTGCTGCAACGCTGCCTCGACGC
GGGGCGCGCGTACGGGCTGCGGCTGGCGGAGCCGGGAGAATTCACCCGCCGCGCGTTTCTGAACGACAAGCTCGATCTTG
CACAGGCGGAGGCCGTTGCCGATCTGATCGAGGCGAGCACCGAGGCGGCGGCGCGCTCGGCGGGACGCTCGCTCGACGGC
GCGTTCTCGCGCGACATCCATGCGCTCGTCGACGACGTGATCGCGCTGCGGATGCTCGTCGAGGCGACGCTCGATTTTCC
GGAGGAAGAGATCGACTTTCTCGAGGCGGCGGACGCGCGCGGCAAGCTCGCGCACATCCGCGAGCGGCTCGCGCACGTGC
TCGGCGACGCGCGGCAGGGGGCGTTGTTGCGCGAGGGGCTGTCGGTGGTGCTCGCCGGGCAGCCGAACGTCGGCAAGTCG
TCGCTATTGAATGCGCTTGCGGGGGCGGAGCTGGCGATCGTCACGCCGATCGCGGGCACGACGCGCGACAAGGTCGCGCA
GACGATCCAGGTCGAGGGCATTCCGCTGCACATCATCGATACGGCGGGCTTGCGCGAGACGGAGGACGAGGTCGAGAAGA
TCGGCATCGCGCGCACGTGGGGCGAGATCGAGCGGGCCGACGTCGTGCTGCATCTGCTCGATGCGAGGAGCGGACTCGGC
CCGGACGACGAGGCGATCGCCGCGCGCTTTCCGGCCGGCGTGCCGGTGGTGCGCGTGTTGAACAAGACGGATCTGACGGA
GGCGCCGGCATCGGTGGCGCGGGTGGGTAGCGGTGCGGAGCGCGCGGATCTGTGCGAAGTGCGGCTGTCGGCGAAGCGCG
GCGACGGGATCGATTTGCTGCGCGGCGAACTGCTGCGGATCGCCGGGTGGCAGGCGGGCGCGGAGAGCGTGTATCTCGCG
CGCGAGCGGCATCTGATTGCACTGCGAGCGGCGCAGGCGCATGTCGCGCGGGCGGCCGAGCATGCGGACCAGAATGCTCA
GGCGCTCGATCTCTTCGCCGAGGAACTTCGGCTTGCGCAGGAGCAGCTCAATTCGATCACTGGGGAGTTTTCGTCCGATG
ATTTGCTAGGGGTGATTTTTAGTCGGTTTTGTATCGGAAAATAA

Upstream 100 bases:

>100_bases
TGTCTGCGCAATACTATTGCGTGGCCGGGCTACAATTCGGCATTCAAGGATATCTGCGATTTTTCTTGATTTCCATTTCA
TTTATTTCAGAATTTTTGCC

Downstream 100 bases:

>100_bases
GTAGTCGCCAGCCGTCACCTAAGTTTCACAAGAAAGAAGCCGCAAAGCCCCGCCAGACATAGATCGACTCCCCATATTCC
CCAAAGTTTACCAATCGACA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLATDSDPIVAIATASGRGGIGVVRISLGRAGEAAALALSDALCGARLTPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCLDAGRAYGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PDDEAIAARFPAGVPVVRVLNKTDLTEAPASVARVGSGAERADLCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHVARAAEHADQNAQALDLFAEELRLAQEQLNSITGEFSSDDLLGVIFSRFCIGK

Sequences:

>Translated_467_residues
MLATDSDPIVAIATASGRGGIGVVRISLGRAGEAAALALSDALCGARLTPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCLDAGRAYGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PDDEAIAARFPAGVPVVRVLNKTDLTEAPASVARVGSGAERADLCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHVARAAEHADQNAQALDLFAEELRLAQEQLNSITGEFSSDDLLGVIFSRFCIGK
>Mature_467_residues
MLATDSDPIVAIATASGRGGIGVVRISLGRAGEAAALALSDALCGARLTPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCLDAGRAYGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PDDEAIAARFPAGVPVVRVLNKTDLTEAPASVARVGSGAERADLCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHVARAAEHADQNAQALDLFAEELRLAQEQLNSITGEFSSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=481, Percent_Identity=37.8378378378378, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=511, Percent_Identity=35.812133072407, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI193082993, Length=471, Percent_Identity=37.5796178343949, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=53.6324786324786, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082120, Length=117, Percent_Identity=45.2991452991453, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=429, Percent_Identity=29.6037296037296, Blast_Score=147, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323665, Length=426, Percent_Identity=30.9859154929577, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45550813, Length=497, Percent_Identity=33.4004024144869, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49465; Mature: 49465

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATDSDPIVAIATASGRGGIGVVRISLGRAGEAAALALSDALCGARLTPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GAGEPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVLQLVLQRCLDAGRAYGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVDDVIALRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDARSGLGPDDEAIAARFPAGVPVVRVLNKTDLTEAPASVARVGSGAE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCC
RADLCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLARERHLIALRAAQAHVARAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHCCCEEEEHHHHHHHHHHHH
HADQNAQALDLFAEELRLAQEQLNSITGEFSSDDLLGVIFSRFCIGK
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATDSDPIVAIATASGRGGIGVVRISLGRAGEAAALALSDALCGARLTPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
GAGEPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVLQLVLQRCLDAGRAYGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVDDVIALRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDARSGLGPDDEAIAARFPAGVPVVRVLNKTDLTEAPASVARVGSGAE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCC
RADLCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLARERHLIALRAAQAHVARAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHCCCEEEEHHHHHHHHHHHH
HADQNAQALDLFAEELRLAQEQLNSITGEFSSDDLLGVIFSRFCIGK
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA