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The map label for this gene is hyfE

Identifier: 83591012

GI number: 83591012

Start: 2288290

End: 2288940

Strand: Reverse

Name: hyfE

Synonym: Moth_2189

Alternate gene names: 83591012

Gene position: 2288940-2288290 (Counterclockwise)

Preceding gene: 83591013

Following gene: 83591011

Centisome position: 87.07

GC content: 48.23

Gene sequence:

>651_bases
GTGTCCGGCACTAGTGTAGTCAATACCCTGGCTGTACTGCTCATCCTGACCTCGATGCTGGTGGTGGAAACCAGGAAGCT
CCGGATGGCAGCATATATGTACAGCATCCAGTCCCTGGTTCTGGTGGCGATTTTTCTGTCCCTGGCGGTATTTATGCATG
CCGAACCATTGTACATCTGGTCGATTACGGCACTCATCACCAAGACCTTCCTGGTGCCATACCTAATACTCAGGACTATT
AAAAAGGTAGGAGACCAGGAGGAAACCGGGCTGGCCCTCACAACAGCAACGTCTGTTTTTCTGGCGGCGTGTATGGTTGC
TTTATCTTTTGTTATAGTAGAACCTTTCCAGGTCCAGGCCATACTGAAATTGAAGGTTGCCCTGGCAGTTTCGTTAGCTC
ATTTTTTACTCGGCGTTCTCTGTATACTTGCCAGGAAGAATGCGCTCAAGCAGATTTTGGGCTACTGCCTGATGGAGAAC
GGATCCCATTTGACCCTTGCAATCATGGCCTATAACGCGCCTGAAACAGTGGAGATAGGTATCCTGACTGACGCTGTTTT
TGCCGTGATTATCTTGACGATTATTGCCAGGAGACTCTTTAAAGCCTTCAACACCCTGGACACGGATAAACTTACATTAT
TAAAGGGTTAG

Upstream 100 bases:

>100_bases
ATTTATAAGGCCCCTGAAGTGACCTGGCTGGCTTTCGGTATAGCCCTGCTGTCCTTCGTTTTCTACCTGGTCAATGTTTA
AGAGTGGAGGGGATAAGGTA

Downstream 100 bases:

>100_bases
GTGATAGGAGTGGGGGAGAATATTTTATTCTACTTGCCCGCGGTACCGCTAGGAATAGCCGTGCTGGCTTTTGGTATAAG
GTCGCGCCGTATTGTAGAGC

Product: hydrogenase 4 membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSGTSVVNTLAVLLILTSMLVVETRKLRMAAYMYSIQSLVLVAIFLSLAVFMHAEPLYIWSITALITKTFLVPYLILRTI
KKVGDQEETGLALTTATSVFLAACMVALSFVIVEPFQVQAILKLKVALAVSLAHFLLGVLCILARKNALKQILGYCLMEN
GSHLTLAIMAYNAPETVEIGILTDAVFAVIILTIIARRLFKAFNTLDTDKLTLLKG

Sequences:

>Translated_216_residues
MSGTSVVNTLAVLLILTSMLVVETRKLRMAAYMYSIQSLVLVAIFLSLAVFMHAEPLYIWSITALITKTFLVPYLILRTI
KKVGDQEETGLALTTATSVFLAACMVALSFVIVEPFQVQAILKLKVALAVSLAHFLLGVLCILARKNALKQILGYCLMEN
GSHLTLAIMAYNAPETVEIGILTDAVFAVIILTIIARRLFKAFNTLDTDKLTLLKG
>Mature_215_residues
SGTSVVNTLAVLLILTSMLVVETRKLRMAAYMYSIQSLVLVAIFLSLAVFMHAEPLYIWSITALITKTFLVPYLILRTIK
KVGDQEETGLALTTATSVFLAACMVALSFVIVEPFQVQAILKLKVALAVSLAHFLLGVLCILARKNALKQILGYCLMENG
SHLTLAIMAYNAPETVEIGILTDAVFAVIILTIIARRLFKAFNTLDTDKLTLLKG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=216, Percent_Identity=46.7592592592593, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001133 [H]

Pfam domain/function: PF00420 Oxidored_q2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23623; Mature: 23492

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTSVVNTLAVLLILTSMLVVETRKLRMAAYMYSIQSLVLVAIFLSLAVFMHAEPLYIW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SITALITKTFLVPYLILRTIKKVGDQEETGLALTTATSVFLAACMVALSFVIVEPFQVQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ILKLKVALAVSLAHFLLGVLCILARKNALKQILGYCLMENGSHLTLAIMAYNAPETVEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEH
ILTDAVFAVIILTIIARRLFKAFNTLDTDKLTLLKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SGTSVVNTLAVLLILTSMLVVETRKLRMAAYMYSIQSLVLVAIFLSLAVFMHAEPLYIW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SITALITKTFLVPYLILRTIKKVGDQEETGLALTTATSVFLAACMVALSFVIVEPFQVQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ILKLKVALAVSLAHFLLGVLCILARKNALKQILGYCLMENGSHLTLAIMAYNAPETVEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEH
ILTDAVFAVIILTIIARRLFKAFNTLDTDKLTLLKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]