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The map label for this gene is hyfF [H]

Identifier: 83591011

GI number: 83591011

Start: 2286802

End: 2288289

Strand: Reverse

Name: hyfF [H]

Synonym: Moth_2188

Alternate gene names: 83591011

Gene position: 2288289-2286802 (Counterclockwise)

Preceding gene: 83591012

Following gene: 83591010

Centisome position: 87.05

GC content: 50.0

Gene sequence:

>1488_bases
GTGATAGGAGTGGGGGAGAATATTTTATTCTACTTGCCCGCGGTACCGCTAGGAATAGCCGTGCTGGCTTTTGGTATAAG
GTCGCGCCGTATTGTAGAGCTTTTGCACCTTGCGGGGATAACTATCGTAGCTTTTCTCTCTTTGTTGCTGGTACGCAGCG
TTTTAAGGGGTCAAACGCTCTTTGCTCTTAATCAAATGCTCTATGCAGACAGCCTGACGGCGATTCTGGTCCTTATTATT
GGGGTACTGGGATTTCTAGATGGATTTTACTCTATAGGATACCTGCGACACGATCTAGAACATGGTGAAGCAGATGAGAG
GAGCGTCTGCTCTTATTACGGTTTCTTTCATATATTTTTGTTCACCATGCTTCTGTCCGCTATATCGAACAATATTGCCA
TTATGTGGGCTGCGGTTGAAGCAACCACCTTGGGCTCGGCCTTTTTGGTCGGGTTTTACAAGTACAAGACTTCGGCTGAA
GGTGCCTGGAAGTACGTCCTGATTTGTAGTGTTGGCCTGGCCTTTGCTTTATACGGAACTATCCTGACTTATTCCGATGC
CTTTACCATATTGCAAGATGCTCACCGGGCGATGCTGTGGACGGAGATAGCGGGGATGGCCCATAGCCTTGATCCGCAGG
TAATGAAACTGGCCTTTGTGTTTATTTTACTCGGTTTCGGCACCAAGGCCGGCCTGTTTCCTATGCATACCTGGCTGCCC
GATGCCCACAGCGAGGCCCCGAGCCCTGCCAGTGCCATGCTGTCGGGTGTGCTGCTAAAGTGTGCCCTGTTTGCGATAAT
AAGATACTATGCCATTGCCTTACAGGTTCTAGGGAGGAGCTTCCCCCAAACCCTACTCCTTATTTTCGGTCTTCTCTCTG
TATGCGTAGCGGCCTTCTTTATACTGGTGCAAAACGATATCAAGAGGAAACTGGCTTACAGCAGTGTCGAGCATATCGGT
ATCATCACTACAGGTTTGGGCGTGGGCGGTGCTCTGGGTATATTTGCTGCCCTTCTTCATGCCATCAATCATAGTATTAC
CAAGTCCCTTTTGTTTTGTGCCTCAGGAAATGTTGTTATTAAGTATGGCACCAGGGATTCCCAGAAAATCCGGGGTCTGC
TTAAGGTGGCTCCCTTTACCGGGTTGATGTTCATGGCAGGGCTCCTGACAGTAGCAGGTTCACCACCCCTCAATATCTTC
GTCAGTGAGTTTATGACCCTGACGGCCGGGCTCAAGGGTGGCTATCTCTGGCCGATGATACTGTTGCTTATCGCCCTGGT
GATTGTTTTTGCCGCGCTAGTTATCCTGATAAGCGGTTCGGTTTTCGGTTCCCCTCCGGAAGGTCTATCCCGGGGTGAGG
TCGGGTGGCTGACCCTGCTGCCTTTTGGGATTCTGTTCCTGCTGATGGCCGGATTGGGGATATATATACCTGCACCGTTG
AATCAATTGCTGCAGAGTGCAGTTAACGTTGTCCTGGGAGCGAGGTAA

Upstream 100 bases:

>100_bases
CGCTGTTTTTGCCGTGATTATCTTGACGATTATTGCCAGGAGACTCTTTAAAGCCTTCAACACCCTGGACACGGATAAAC
TTACATTATTAAAGGGTTAG

Downstream 100 bases:

>100_bases
TAAATATGGTAGAATATGCCGTAGCAAGCATTTTAATCCCCATACTGGGAAGCGTCTTGGCTGTAATCATGCCAGGCAGC
TATGTAAAGAGAATAAGCCC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MIGVGENILFYLPAVPLGIAVLAFGIRSRRIVELLHLAGITIVAFLSLLLVRSVLRGQTLFALNQMLYADSLTAILVLII
GVLGFLDGFYSIGYLRHDLEHGEADERSVCSYYGFFHIFLFTMLLSAISNNIAIMWAAVEATTLGSAFLVGFYKYKTSAE
GAWKYVLICSVGLAFALYGTILTYSDAFTILQDAHRAMLWTEIAGMAHSLDPQVMKLAFVFILLGFGTKAGLFPMHTWLP
DAHSEAPSPASAMLSGVLLKCALFAIIRYYAIALQVLGRSFPQTLLLIFGLLSVCVAAFFILVQNDIKRKLAYSSVEHIG
IITTGLGVGGALGIFAALLHAINHSITKSLLFCASGNVVIKYGTRDSQKIRGLLKVAPFTGLMFMAGLLTVAGSPPLNIF
VSEFMTLTAGLKGGYLWPMILLLIALVIVFAALVILISGSVFGSPPEGLSRGEVGWLTLLPFGILFLLMAGLGIYIPAPL
NQLLQSAVNVVLGAR

Sequences:

>Translated_495_residues
MIGVGENILFYLPAVPLGIAVLAFGIRSRRIVELLHLAGITIVAFLSLLLVRSVLRGQTLFALNQMLYADSLTAILVLII
GVLGFLDGFYSIGYLRHDLEHGEADERSVCSYYGFFHIFLFTMLLSAISNNIAIMWAAVEATTLGSAFLVGFYKYKTSAE
GAWKYVLICSVGLAFALYGTILTYSDAFTILQDAHRAMLWTEIAGMAHSLDPQVMKLAFVFILLGFGTKAGLFPMHTWLP
DAHSEAPSPASAMLSGVLLKCALFAIIRYYAIALQVLGRSFPQTLLLIFGLLSVCVAAFFILVQNDIKRKLAYSSVEHIG
IITTGLGVGGALGIFAALLHAINHSITKSLLFCASGNVVIKYGTRDSQKIRGLLKVAPFTGLMFMAGLLTVAGSPPLNIF
VSEFMTLTAGLKGGYLWPMILLLIALVIVFAALVILISGSVFGSPPEGLSRGEVGWLTLLPFGILFLLMAGLGIYIPAPL
NQLLQSAVNVVLGAR
>Mature_495_residues
MIGVGENILFYLPAVPLGIAVLAFGIRSRRIVELLHLAGITIVAFLSLLLVRSVLRGQTLFALNQMLYADSLTAILVLII
GVLGFLDGFYSIGYLRHDLEHGEADERSVCSYYGFFHIFLFTMLLSAISNNIAIMWAAVEATTLGSAFLVGFYKYKTSAE
GAWKYVLICSVGLAFALYGTILTYSDAFTILQDAHRAMLWTEIAGMAHSLDPQVMKLAFVFILLGFGTKAGLFPMHTWLP
DAHSEAPSPASAMLSGVLLKCALFAIIRYYAIALQVLGRSFPQTLLLIFGLLSVCVAAFFILVQNDIKRKLAYSSVEHIG
IITTGLGVGGALGIFAALLHAINHSITKSLLFCASGNVVIKYGTRDSQKIRGLLKVAPFTGLMFMAGLLTVAGSPPLNIF
VSEFMTLTAGLKGGYLWPMILLLIALVIVFAALVILISGSVFGSPPEGLSRGEVGWLTLLPFGILFLLMAGLGIYIPAPL
NQLLQSAVNVVLGAR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=371, Percent_Identity=27.4932614555256, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI251831116, Length=196, Percent_Identity=29.0816326530612, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788831, Length=475, Percent_Identity=54.1052631578947, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1788827, Length=355, Percent_Identity=30.9859154929577, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788829, Length=396, Percent_Identity=28.5353535353535, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788614, Length=325, Percent_Identity=29.8461538461538, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI2367154, Length=356, Percent_Identity=28.0898876404494, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788613, Length=414, Percent_Identity=25.1207729468599, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693160, Length=301, Percent_Identity=25.2491694352159, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53298; Mature: 53298

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGVGENILFYLPAVPLGIAVLAFGIRSRRIVELLHLAGITIVAFLSLLLVRSVLRGQTL
CCCCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FALNQMLYADSLTAILVLIIGVLGFLDGFYSIGYLRHDLEHGEADERSVCSYYGFFHIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FTMLLSAISNNIAIMWAAVEATTLGSAFLVGFYKYKTSAEGAWKYVLICSVGLAFALYGT
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ILTYSDAFTILQDAHRAMLWTEIAGMAHSLDPQVMKLAFVFILLGFGTKAGLFPMHTWLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
DAHSEAPSPASAMLSGVLLKCALFAIIRYYAIALQVLGRSFPQTLLLIFGLLSVCVAAFF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILVQNDIKRKLAYSSVEHIGIITTGLGVGGALGIFAALLHAINHSITKSLLFCASGNVVI
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEE
KYGTRDSQKIRGLLKVAPFTGLMFMAGLLTVAGSPPLNIFVSEFMTLTAGLKGGYLWPMI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
LLLIALVIVFAALVILISGSVFGSPPEGLSRGEVGWLTLLPFGILFLLMAGLGIYIPAPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCH
NQLLQSAVNVVLGAR
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGVGENILFYLPAVPLGIAVLAFGIRSRRIVELLHLAGITIVAFLSLLLVRSVLRGQTL
CCCCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FALNQMLYADSLTAILVLIIGVLGFLDGFYSIGYLRHDLEHGEADERSVCSYYGFFHIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FTMLLSAISNNIAIMWAAVEATTLGSAFLVGFYKYKTSAEGAWKYVLICSVGLAFALYGT
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ILTYSDAFTILQDAHRAMLWTEIAGMAHSLDPQVMKLAFVFILLGFGTKAGLFPMHTWLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
DAHSEAPSPASAMLSGVLLKCALFAIIRYYAIALQVLGRSFPQTLLLIFGLLSVCVAAFF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILVQNDIKRKLAYSSVEHIGIITTGLGVGGALGIFAALLHAINHSITKSLLFCASGNVVI
HHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEE
KYGTRDSQKIRGLLKVAPFTGLMFMAGLLTVAGSPPLNIFVSEFMTLTAGLKGGYLWPMI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
LLLIALVIVFAALVILISGSVFGSPPEGLSRGEVGWLTLLPFGILFLLMAGLGIYIPAPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCH
NQLLQSAVNVVLGAR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]