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The map label for this gene is mtbC3 [H]

Identifier: 83590158

GI number: 83590158

Start: 1355270

End: 1355935

Strand: Reverse

Name: mtbC3 [H]

Synonym: Moth_1311

Alternate gene names: 83590158

Gene position: 1355935-1355270 (Counterclockwise)

Preceding gene: 83590159

Following gene: 83590157

Centisome position: 51.58

GC content: 56.01

Gene sequence:

>666_bases
ATGAGCCTCTTGGCCCAGGCTATGGGAAAATTGGAAGAAAAAAGGGTACTGGAGCTTGTCAGCCACAGCCTTGCGGTGGG
ACAACCGCCCCTGGCAATTGTAGAAGAATGCCGTCGGGGTTTGAATATAGTTGGCGAGCTTTACGCCCGCGGTGAGTATT
TTTTGAGCGATCTGGTCATGTCGGCGGAGATTTTCCAGGAAGCCTTAAAACTTATCGAGCCTTTTCTGAAAGTACCCCTG
GCAGATAGGCAGGAACCGGCCGAAGCAGATATTGTCTTTGGTACAGTTGAAGGGGACATTCACGACATCGGCAAAAATAT
CACTATCTCCCTGCTGCGCTGCGAGGGTTTCCGGGTCTATGACGCAGGGGTGGATATACCGCCTGACGTTTTTCTAACTC
TAATCCAGAAGACCGGGGCCCGGGTCCTTGGTCTCTCGGCCCTGCTGACCTCTTCCTTTGAGGCTATGCGCCGTACCGTT
CACCTGATGCACCTGGTGGAAAGCATGGCCCCGGTCAAGGTCCTCATCGGCGGCCTGGTCAATGAGCGCGTCTGCCAGTA
CGTCGGCGCCGACGCCTGGGTCCGGGAAGCCGGGGAAGGGGTGGCCATCTGTCGCCGCTGGCTACGGCATGAACGGGCTG
CCAGAATAGCTACCGATGTAAACTAA

Upstream 100 bases:

>100_bases
CGGTGGGGTGGGGATGGTGCCTGTACCGCGGCGACAGGTACCAGTTAAGCTCCCAGTCGCCTATTTTCTAATTTACACCA
AAAATCTGGAGGTGGACCGG

Downstream 100 bases:

>100_bases
ACCCATAGCAGTTGAGGTCTGATATAAACTATAAGAAGGGCGGCATAAAATGAATAAAGGCCGTTTTGCAAGGCCGCGCC
GGGGAGGCTATTTACCCCTT

Product: cobalamin B12-binding

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSLLAQAMGKLEEKRVLELVSHSLAVGQPPLAIVEECRRGLNIVGELYARGEYFLSDLVMSAEIFQEALKLIEPFLKVPL
ADRQEPAEADIVFGTVEGDIHDIGKNITISLLRCEGFRVYDAGVDIPPDVFLTLIQKTGARVLGLSALLTSSFEAMRRTV
HLMHLVESMAPVKVLIGGLVNERVCQYVGADAWVREAGEGVAICRRWLRHERAARIATDVN

Sequences:

>Translated_221_residues
MSLLAQAMGKLEEKRVLELVSHSLAVGQPPLAIVEECRRGLNIVGELYARGEYFLSDLVMSAEIFQEALKLIEPFLKVPL
ADRQEPAEADIVFGTVEGDIHDIGKNITISLLRCEGFRVYDAGVDIPPDVFLTLIQKTGARVLGLSALLTSSFEAMRRTV
HLMHLVESMAPVKVLIGGLVNERVCQYVGADAWVREAGEGVAICRRWLRHERAARIATDVN
>Mature_220_residues
SLLAQAMGKLEEKRVLELVSHSLAVGQPPLAIVEECRRGLNIVGELYARGEYFLSDLVMSAEIFQEALKLIEPFLKVPLA
DRQEPAEADIVFGTVEGDIHDIGKNITISLLRCEGFRVYDAGVDIPPDVFLTLIQKTGARVLGLSALLTSSFEAMRRTVH
LMHLVESMAPVKVLIGGLVNERVCQYVGADAWVREAGEGVAICRRWLRHERAARIATDVN

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=197, Percent_Identity=31.4720812182741, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1790450, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535405, Length=165, Percent_Identity=32.7272727272727, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24392; Mature: 24261

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLAQAMGKLEEKRVLELVSHSLAVGQPPLAIVEECRRGLNIVGELYARGEYFLSDLVM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEIFQEALKLIEPFLKVPLADRQEPAEADIVFGTVEGDIHDIGKNITISLLRCEGFRVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHCCCCCEEEEEEECCEEEE
DAGVDIPPDVFLTLIQKTGARVLGLSALLTSSFEAMRRTVHLMHLVESMAPVKVLIGGLV
ECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NERVCQYVGADAWVREAGEGVAICRRWLRHERAARIATDVN
HHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLLAQAMGKLEEKRVLELVSHSLAVGQPPLAIVEECRRGLNIVGELYARGEYFLSDLVM
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAEIFQEALKLIEPFLKVPLADRQEPAEADIVFGTVEGDIHDIGKNITISLLRCEGFRVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHCCCCCEEEEEEECCEEEE
DAGVDIPPDVFLTLIQKTGARVLGLSALLTSSFEAMRRTVHLMHLVESMAPVKVLIGGLV
ECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NERVCQYVGADAWVREAGEGVAICRRWLRHERAARIATDVN
HHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]